Photon counting multi-channel time-resolved fluorescence immunoassay system and counting method

Abstract

The invention belongs to the technical field of fluorescence immunoassay and relates to a photon counting multi-channel time-resolved fluorescence immunoassay system which comprises a light source module, a detection module and a time marked photon counting analysis/control module. At the same time, the invention relates to a counting method adopted by the system. The counting method of exciting a sample to be detected with pulse light, sending a fluorescence signal generated by the sample after excitation into a PMT (Photo Multiplier Tube) photon counting head and outputting a pulse signal comprises the following steps of taking cycles of the excitation pulse light as counting cycles, dividing each counting cycle into a plurality of time intervals with the same width, accumulating photons detected after sending excitation light pulses of each counting cycle into a memory corresponding to a corresponding time window, that is marking the positions of the photons in the pulse cycles with the intervals where the time windows are located, and uploading data in the memory into an upper computer after counting for the cycles. The multi-channel time-resolved fluorescence immunoassay system is low in cost and has very high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of fluorescence immunoassay, and in particular relates to a fluorescence immunoassay system. Background technique [0002] Time-resolved Fluoroimmunoassay (TRFIA) technology has been proved to be an effective tool in the luminescence analysis of biomacromolecules, which uses trivalent rare earth ions and their chelates as long-lived fluorescent Tracers are used to label substances such as antigens, antibodies, and nucleic acid probes. After the immune reaction occurs, according to the fluorescence spectrum characteristics of the rare earth ion chelate, the time-resolved fluorescence detection system is used to reduce the influence of background fluorescence through delayed measurement, and the fluorescence intensity and lifetime of the final product of the immune reaction are measured to achieve high-confidence Luminescence analysis of noise ratio. Compare the fluorescence intensity of the substance to be ...

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Problems solved by technology

Both of these methods can achieve picosecond or even femtosecond time resolution,

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