Test strip, kit and method for detecting AMH (anti-mullerian hormone) with TRFIA (time-resolved fluorescence immunoassay)

A technology of time-resolved fluorescence and immunochromatography, which is applied in the analysis of materials, biological testing, fluorescence/phosphorescence, etc., can solve the problems of high requirements for detection equipment, long detection time, and poor repeatability, so as to avoid fluorescence interference, The effect of high accuracy and strong specificity

Pending Publication Date: 2018-06-22
贵州立知健生物科技有限公司
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  • Abstract
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Problems solved by technology

[0009] The methods currently used to detect AMH mainly include enzyme-linked immunosorbent assay (ELISA), electrochemiluminescence immunoassay (ECLI), chemiluminescence immunoassay (CLI), etc., but these methods All have the following defects: high requirements for testing equipment, high cost; many interference factors, poor repeatability; long testing time
[0012] However, in the prior art, there is a lack of a fast, convenient, and economical kit and method for the dry fluorescent immunoassay analyzer using TRFIA, so that it can meet the requirements of single-serving and small-batch detection of AMH

Method used

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  • Test strip, kit and method for detecting AMH (anti-mullerian hormone) with TRFIA (time-resolved fluorescence immunoassay)
  • Test strip, kit and method for detecting AMH (anti-mullerian hormone) with TRFIA (time-resolved fluorescence immunoassay)
  • Test strip, kit and method for detecting AMH (anti-mullerian hormone) with TRFIA (time-resolved fluorescence immunoassay)

Examples

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Effect test

Embodiment 1

[0054] Example 1: Preparation of a kit for detecting AMH by time-resolved fluorescent immunochromatography

[0055] The kit for detecting AMH by time-resolved fluorescence immunochromatography uses the principle of double-antibody sandwich immunochromatography to detect AMH content in human whole blood. The kit consists of a time-resolved fluorescent immunochromatography test strip for detecting AMH, a sample buffer and an ID card containing the AMH standard curve. In this example, if figure 1 and figure 2 As shown: the whole blood filter pad 2, the antibody carrying membrane 3 (nitrocellulose membrane as the base membrane) and the absorbent paper 4 are successively lapped on the PVC bottom plate 1; the antibody carrying membrane 3 is provided with a microsphere line 5, a detection line 6 and The control line 7; the whole blood filter pad 2 is used as a sample loading area for absorbing the whole blood sample to be tested.

[0056] The mouse anti-human AMH monoclonal antib...

Embodiment 2

[0069] Embodiment 2: Time-resolved fluorescent immunochromatography detects AMH concentration

[0070] Add different concentrations of human AMH antigen calibrator (16.0ng / ml, 8.0ng / ml, 4.0ng / ml, 2.0ng / ml, 1.0ng / ml, 0.5ng / ml in the test strip prepared by Example 1) , 0.1ng / ml, 0.0ng / ml, a total of eight concentrations, each concentration set three replicates, all of which were prepared by diluting 1.0mg / ml human AMH antigen with sample buffer), after 15min of chromatography, passed through Jiangsu Suzhou and The FIC-S1 dry fluorescent immunoassay analyzer produced by Mai Precision Instrument Biopharmaceutical Co., Ltd. reads the fluorescence intensity ratio of the test line and the control line (detection line detection value / control line detection value).

[0071] Take the concentration of AMH calibrator as the X-axis, and take the fluorescence intensity of the sample detection line / fluorescence intensity of the control line as the Y-axis to establish an equation and fit it i...

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Abstract

The invention relates to a test strip, a kit and a method for detecting AMH (anti-mullerian hormone) with TRFIA (time-resolved fluorescence immunoassay). The test strip comprises a PVC bottom plate, awhole blood filter pad, an antibody carrying membrane and absorbent paper. The kit comprises the test strip for detecting the AMH with the TRFIA, a sample buffer solution and an ID card containing anAMH standard curve. The kit can meet the requirement of single-person and small-batch detection of AMH and has the advantages of high sensitivity, high specificity, small blood amount, short detection time, operation convenience and detection result accuracy and reliability.

Description

technical field [0001] The invention relates to the technical field of hormone detection, in particular to a test strip, a kit and a method for detecting AMH by time-resolved fluorescence immunochromatography. Background technique [0002] Anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting hormone MIH, is a structurally related member of the transforming growth factor-β (TGF-β) superfamily Inhibin and activin-related glycoprotein hormones whose main role is to regulate growth, differentiation and folliculogenesis. In male embryos, AMH is activated by the SOX9 gene in testicular Sertoli cells of male fetuses, whose expression inhibits the development of the female reproductive tract or Müllerian ducts (paramesonephric ducts), thereby inhibiting the production of fallopian tubes, uterus, and vagina. The expression of AMH is critical for sex differentiation during specific periods of embryonic development. In women, AMH is secreted by preantral granulosa cells a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/533G01N33/558G01N33/577G01N33/74
CPCG01N21/6486G01N33/533G01N33/558G01N33/577G01N33/74G01N2333/575
Inventor 李翀康星张旭范祖森闫慧娟
Owner 贵州立知健生物科技有限公司
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