Double rare earth coordination compound, Ag at SiO2 fluorescent nano particle doped with the same and preparation method thereof

Abstract

The invention relates to a double rare earth coordination compound, an Ag at SiO2 fluorescent nano particle doped with the same and a preparation method thereof. The fluorescent nano particle takes Ag doped with the double rare earth coordination compound Eu3<+> / Tb3<+>-PABA-DTPA-APTMS as an inner core; silicon dioxide with a mesh structure is covered on the surface of the inner core; an active amino group is arranged on the surface of the silicon dioxide, wherein the ratio of the double rare earth coordination Eu3<+> / Tb3<+>-PABA-DTPA-APTMS to the Ag is 1:0.176-0.2; and the mass ratio of the inner core to the silicon dioxide is 1:5-12 and each milligram of nano particle contains 595-630nmol of amino groups. The fluorescence intensity of Eu3<+> and Tb3<+> in the nano particle at the maximum transmitting peak is improved by 3.0 and 3.4 times compared with the fluorescence intensity of a SiO2 fluorescent nano particle doped with the Eu3<+> / Tb3<+>-PABA-DTPA-APTMS without an Ag core; the prepared nano particle is regularly spherical, is uniform in size with the particle size of 120+ / -5nm and has favorable monodispersity and light stability; and an amino group is arranged on the surface of the nano particle and directly reacts with a biomolecule without surface modification. The nano particle is expected to be used as a novel rare earth fluorescent probe which is applied to the time distinguishing fluorescent immunoassay for high-sensitivity detection, a biosensor, a biological chip and the like.

Description

technical field [0001] The present invention relates to a kind of Ag@SiO 2 Fluorescent nanoparticles and methods for their preparation. In particular, a double rare earth complex, its doped Ag@SiO 2 Fluorescent nanoparticles and methods for their preparation. Background technique [0002] Rare earth complexes have the characteristics of long-lived fluorescence and large Stokes shift. In recent years, they have been prepared into core-shell silicon nanoparticles with good water solubility, good biocompatibility, and easy surface modification and applied in time-resolved fluorescence immunoassay. , DNA detection and cell imaging [5] etc. have become a research hotspot of rare earth compounds. [0003] Among the rare earth complex fluorescent nanoparticles prepared so far, most of the rare earth complexes wrapped in nanoparticles contain only one kind of rare earth ions, while fluorescent nanoparticles containing two or more rare earth ions are rarely reported. Different r...

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Problems solved by technology

[0003] Among the rare earth complex fluorescent nanoparticles prepared so far, most of the rare earth complexes wrapped in nanoparticles contain only one kind of rare earth ion, while fluorescent nanoparticles containing two or more rare earth ions are rarely reported.

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