Reagent box for detecting aftatoxin B and detecting method thereof

A kind of technology of aflatoxin and reagent kit, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complicated operation, expensive instruments and equipment, low sensitivity, etc., and achieve the effect of convenient use, simple structure and high sensitivity
CN1673747AInactive Publication Date: 2005-09-28JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2005-09-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The aflatoxin B1 detecting kit and its detecting method belongs to the field of time-resolved fluorescent immunoassay (TRFIA) technology, and is used in the detection of aflatoxin B1 (AFB1) content in food and feed. The kit of the present invention detects AFB1 in TRFIA based on marker immune reaction. The microporous board has coated AFB1-HRP, added AFB1 standard or sample, and added AFB1 antibody. The free AFB1 and the AFB1-HRP in the microporous board compete AFB1 antibody, un-connected AFB1 antibody is washed off, EU3+ sheep anti-rabbit antibody is added and un-connected EU3+ sheep anti-rabbit antibody after marker immune reaction is washed off. After reinforcing solution is added, the fluorescence strength cps, which is proportional inversely to AFB1 concentration in the sample, is determined with time-resolved fluorescent instrument to determine the AFB1 content in the tested sample via comparison with standard curve.
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Description

technical field

[0001] A method for detecting aflatoxin B 1 The kit and detection method thereof belong to the technical field of time-resolved fluorescent immunoassay (TRFIA), and are used for detecting aflatoxin B in grain, feed and food thereof 1 (referred to as AFB 1 ) content detection. Background technique

[0002] Aflatoxin B 1 (AFB 1 ) is a group of highly toxic secondary metabolites produced by the fungi Hspergillus flavus and A. parasilicus strains, especially AFB 1 It is a strong pollutant with a wide distribution range, which can cause acute poisoning death of humans and various feed animals, and can also cause carcinogenicity, teratogenicity, and mutagenicity. Even the content of tens of μg / kg is still very toxic. More than 1mg / kg of aflatoxin in food and feed is highly toxic. Its toxicity is 10 times that of potassium cyanide and 68 times that of arsenic. After eating food seriously contaminated by aflatoxin, fever, abdominal pain, vomiting, and loss of ...

Claims

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