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Method for detecting imidacloprid by quantum-dot-marked sandwich fluorescence immunoassay

A fluorescent immunoassay and quantum dot technology, applied in the field of fluorescent immunoassay, can solve the problems of long detection time, sample matrix interference, large gap, etc., and achieve the effect of rapid detection

Inactive Publication Date: 2013-09-25
TIANJIN AGRICULTURE COLLEGE
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Problems solved by technology

[0003] At present, food safety testing methods mainly include chemical analysis (CA), thin layer chromatography (TLC), gas chromatography (GC), high performance liquid chromatography (HPLC), GC-MS, enzyme-linked immunosorbent assay These methods have constraints such as long detection time, low sensitivity, high false positives, complex sample pretreatment, and serious sample matrix interference, which are difficult to meet the needs of actual detection, especially on-site rapid detection.
At this stage, there is a large gap between my country's food safety key detection technology and developed countries, which largely brings hidden dangers to my country's food safety, and also limits my country's food trade

Method used

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Embodiment Construction

[0022] The method for the detection of imidacloprid by the sandwich fluorescent immunoassay labeled with quantum dots is as follows:

[0023] 1. Preparation of core-shell quantum dots and water-soluble modification of fat-soluble quantum dots by different polymer modifications;

[0024] 2. Preparation of quantum dot-labeled imidacloprid antibody;

[0025] 3. Establishment of imidacloprid antibody quantum dot immunoassay method.

[0026] 1. A method for quantum dot-labeled sandwich fluorescent immunodetection of imidacloprid: the preparation of quantum dots selects semiconductor materials commonly used at present: zinc, selenium, cadmium, and silicon are raw materials, and adopt colloidal chemical methods to prepare a stable core-shell structure, Quantum dots that can present different fluorescent colors, each quantum dot has a spectral address; this method adopts two methods of organic phase synthesis and aqueous solution synthesis, and the organic phase synthesis of CdX (X=S...

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Abstract

The invention discloses a method for detecting imidacloprid by quantum-dot-marked sandwich fluorescence immunoassay. According to the technical scheme, the method comprises the following steps of: 1, preparing a nuclear shell quantum dot and modifying the water solubility of a fat-soluble quantum dot by modification of different macromolecules; 2, preparing and passivating a quantum probe; and 3, constructing an imidacloprid antibody quantum dot immunoassay method. The method is applied to preparation, passivation and performance representation of the quantum dot and detection of precipitate imidacloprid residues and is a fluorescence immunoassay method for sensitively and quickly detecting the imidacloprid in vegetables based on the quantum dot and conjugates of IgG-HRP.

Description

technical field [0001] The invention belongs to a fluorescent immunoassay method for detecting imidacloprid in vegetables, in particular to a method for detecting imidacloprid by quantum dot-labeled sandwich fluorescent immunoassay. [0002] The present invention mainly uses quantum dots to mark antibodies to detect pesticide residues. Quantum dots are nanomaterials newly developed in recent years. They have many excellent fluorescence properties and show broad application prospects in the fields of fluorescence immunology, genomics and medicine. The present invention is applied in the preparation, purification, performance characterization of quantum dots and the detection of pesticide imidacloprid residues. It is based on the conjugate of quantum dots and goat anti-mouse secondary antibody, and establishes a sensitive and rapid fluorescent immunoassay for detecting imidacloprid in vegetables. Analysis. Background technique [0003] At present, food safety testing methods ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N21/64
Inventor 崔新仪李宁梁红春曲福玲王学利胡奇
Owner TIANJIN AGRICULTURE COLLEGE
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