Fluorescence immunoassay method of using zinc oxide quantum dots to mark antibody

A determination method and fluorescence immunological technology, applied in fluorescence/phosphorescence, biological testing, measurement devices, etc., can solve the problems of delayed disease discovery and optimal treatment timing, long cycle, poor accuracy, etc., to achieve rapid and convenient testing, conditions Controlled, mildly conditioned effects

Inactive Publication Date: 2009-11-25
南通市京山锦纶有限公司 +1
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Problems solved by technology

The limitation of this method lies in its long period and poor accuracy, which may delay the discovery of the disease and the best time for treatment

Method used

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  • Fluorescence immunoassay method of using zinc oxide quantum dots to mark antibody
  • Fluorescence immunoassay method of using zinc oxide quantum dots to mark antibody

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Embodiment Construction

[0027] (Such as figure 2 )

[0028] Step 1: Add 0.55 g of zinc acetate dihydrate to 25 ml of ethanol, reflux at 80°C for 3 hours, dissolve the zinc acetate, and cool to room temperature.

[0029] Step 2: Add 0.148 g of lithium hydroxide monohydrate to 25 ml of ethanol, and ultrasonically dissolve it for 10 minutes.

[0030] The third step: mix the solution obtained in the first step and the second step, add ethanol to dilute it 10 times, place it in an autoclave, and seal it so that it can withstand a pressure of not less than 2×10 5 Pa, heated to 70° C., kept for 1 hour, naturally cooled to room temperature, taken out, centrifuged, and washed three times with deionized water to obtain zinc oxide quantum dots.

[0031] Step 4: Disperse 5 mg of zinc oxide quantum dots into 100 μl of 5 μg / ml CA19-9 antibody solution, shake at 35° C. for 1 hour, centrifuge, and wash three times with pH7.4 phosphate buffer solution. Finally, it is dispersed in 1 ml of pH7.4 phosphate buffer so...

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Abstract

Fluorescence immunoassay method of using zinc oxide quantum dots to mark antibody is a method of combining zinc oxide quantum dots marked antibody with the antibody fixed on slide through antigen specific reaction, using fluorescent characteristic of the zinc oxide quantum dots to measure fluorescence strength of the zinc oxide fixed on slide, establishing standard curve of relationship between fluorescence strength of the zinc oxide quantum dots and antigen concentration so as to quantificationally determineantigen concentration of actual measured sample. The zinc oxide quantum dots prepared by the invention provided method has uniform particle diameter, good dispensability, stable combination with antibody, reliable and stable testing results and good repeatability, which providing a rapid and reliable detection approach for early detection of disease antigen in medical science clinical.

Description

technical field [0001] The invention belongs to the technical field of immunoassay, and relates to an immunoassay method using zinc oxide quantum dots. Background technique [0002] In addition to its unique semiconductor photoelectric properties, nanostructured zinc oxide is also biosafe and non-toxic, can be chelated with biomolecules such as proteins by hydrogen bonds or other methods and can well maintain its biological activity, and has a high isoelectric point, etc. Excellent performance, which makes it have important applications in biological / chemical sensors. [0003] At present, the detection of low-concentration antigens is an important issue in clinical medicine and diagnostics. Successful detection of low-concentration antigens can provide scientific basis for early diagnosis and treatment of corresponding diseases. The traditional method of antigen concentration detection is to first culture the virus to increase the concentration of the disease antigen, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/53G01N33/552G01N1/28
Inventor 谷保祥徐春祥刘松琴王明亮朱光平
Owner 南通市京山锦纶有限公司
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