Fluorescence immunoassay detection method and kit for HER-2

A fluorescence immunochromatography, HER-2 technology, applied in the field of fluorescence immunochromatography detection method and its detection kit, can solve the problems of long detection time, easy error, complicated operation, etc., and achieve high accuracy and low cost Low, easy-to-operate effect

Inactive Publication Date: 2016-03-16
SUZHOU BIONANOTECH CO LTD
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Problems solved by technology

This method needs to combine different HER-2 antibodies with nano-magnetic beads and phosphatase, and the cost is relatively high; the operation steps include HER-2 fixation, warm bath, su

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  • Fluorescence immunoassay detection method and kit for HER-2
  • Fluorescence immunoassay detection method and kit for HER-2

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Embodiment Construction

[0041] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Apparently, the described embodiments are only a part of the embodiments of the present invention, not all of them, and are not intended to limit the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts fall within the protection scope of the present invention.

[0042] The fluorescent immunochromatographic detection method of HER-2 of the present invention comprises the following steps:

[0043] Step 1) Probe immobilization pad preparation: add the activator 1-(3-dimethylaminopropyl)-3-ethylcarbodiazyme to the solution of fluorescent latex particles with carboxyl or amino groups with an emission wavelength of 500-590nm Amine hydrochloride (EDC) and N-hydroxysulfosuccinimide (Sulfo-NHS) were reacted at room temperature ...

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Abstract

The invention relates to fluorescence immunoassay detection method and kit for HER-2. The method comprises the following steps: preparation of a probe fixing pad; preparation of an immunoassay test strip; preparation of sample diluent; and inspection of the sample. HER-2 monoclonal antibody fluorescence latex particles and goat anti-rabbit IgG fluorescence latex particles are mixed and then diluted by using gold diluent, and the diluted mixture is sprayed on glass fiber so as to prepare the probe fixing pad; and a detection line is coated by an HER-2 monoclonal antibody, and a control line is coated by goat anti-rabbit IgG so as to prepare the fluorescence immunoassay test strip. The detection kit provided by the invention comprises a PVC liner plate, a sample pad, the probe fixing pad, a cellulose nitrate membrane and absorbent paper. The probe fixing pad is prepared by mixing and drying the HER-2 monoclonal antibody fluorescence latex particles and the goat anti-rabbit IgG fluorescence latex particles. Thus, the fluorescence immunoassay detection method and kit have the advantages of high sensitivity, high accuracy, simple operation and low cost.

Description

technical field [0001] The invention relates to a HER-2 detection method and a kit, in particular to a HER-2 fluorescence immunochromatography detection method and a detection kit. Background technique [0002] HER-2 gene, human epidermal growth factor receptor 2 gene, also known as nen, C-erbB-2, Her-2 / neu gene, its expressed protein product is human epidermal growth factor receptor 2, HER-2 gene passes Expression of the HER-2 protein plays a role. The HER-2 proto-oncogene is located on the long arm of chromosome 17. Shih et al. first induced the mouse NIH3T3 cultured cell line with 3-methylcholanthrene and detected it in the transformed neuroblastoma cells. In humans, 20% to 30% of breast cancer cells have overexpression, mainly due to HER-2 gene amplification (95%) or increased transcription (5%). HER-2 is a member of the epidermal growth factor receptor (EGFR) family. When the corresponding extracellular fragment binds to HER-2, it can activate intracellular tyrosine k...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/68
CPCG01N33/577G01N33/68
Inventor 张金林张华廖平璋张鹏
Owner SUZHOU BIONANOTECH CO LTD
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