Quantitative detection and computation method based on fluorescence immuno-chromatographic technology

Abstract

The invention belongs to the field of immuno-chromatographic detection, and in particular relates to a quantitative detection and computation method based on the fluorescence immuno-chromatographic technology, comprising the following steps: inserting a fluorescence immunostrip dripped with a sample solution to be detected into a fluorescence immunoassay instrument, and sequentially performing irradiation with an LED light source, optical filtering processing with an optical filter, detection with a photoelectric detector, electrical signal processing, AD conversion processing, baseline correction, L-M algorithm processing, area computation and concentration computation. The quantitative detection and computation method stresses on signal reconstruction by using an algorithm, eliminates influence of noise and various interferences, ensures the signal processing course undistorted, and can obtain high-precision and low-error results, thereby quickly, conveniently and accurately determining the concentration result.

Description

Technical field [0001] The invention relates to the field of immunochromatographic detection, in particular to a quantitative detection calculation method based on fluorescence immunochromatographic technology. Background technique [0002] With the development of modern technology, more and more portable on-site detection devices have been invented. Portable detection devices can be used in both rapid medical testing and food safety testing. The portable test is also called the on-site rapid test, which is a subdivision of the industry in the continuous development of in vitro diagnostics. Through the on-site sampling and analysis immediately, the complicated test process and the use of laboratory examiners are eliminated. A new method of waiting for a long time to get test results quickly. Among them, fluorescence immunochromatographic detection technology in portable detection is an emerging detection technology produced by the application of fluorescence technology. [0003] ...

AI Technical Summary

Problems solved by technology

However, various influencing factors were not dealt with in the subsequent algorithm processing, and the resulting fluorescent signal may not only contain the emitted light of fluorescent substances, but also include the influence of stray light such as background fluorescence of the reagent plate and electronic circuit interference.

And because the detection line of the reagent strip is very narrow, when the concentration of the detection sample is not high, the light intensity signal emitted by the fluorescent substance is very weak, coupled with the interference of various influencing factors, it will be mixed in the useful signal, or even drown the useful signal , which reduces the signal-to-noise ratio and sensitivity of the

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