Monochromatic fluorescence detection device

Abstract

The invention discloses a monochromatic fluorescence detection device. The monochromatic fluorescence detection device comprises a photoelectric detector, a micropore diaphragm, a focusing lens, a first optical filter, a dichroic mirror, a parabolic reflector, a second optical filter, a collimating lens and an LED light source. The photoelectric detector, the micropore diaphragm, the focusing lensand the first optical filter are sequentially arrayed in an optical axis coincided manner to form a receiving light path. Optical axes of the dichroic mirror and the receiving light path form a 45-degree angle horizontally. The second optical filter, the collimating lens and the LED light source behind the dichronic mirror are sequentially arrayed in an optical axis coincided manner to form an excitation light path. The detection device for temporal separation of C line and T line optical signals on the basis of an optical path conversion structure of a parabolic reflector micromotion mechanism is small in size, high in measurement precision, portable and convenient for onsite detection and can be applied to researching of fluorescence immunoassay quantitative detection instruments.

Description

technical field [0001] The invention relates to a monochrome fluorescence detection device applied to a portable fluorescence immunoassay analyzer, in particular to a monochrome fluorescence detection device which is based on a micro-movement mechanism of a parabolic mirror to switch the optical path of the stripe detection. Background technique [0002] The research and application of fluorescence immunoquantitative detection instruments have been able to realize the quantitative detection of test strips, but most of the instruments currently use a fixed optical mechanism and a motor to drive the kit to translate to complete the optical-mechanical scanning method to obtain C-line and T-line. The strength of the optical signal on the line, this method leads to the large size of the instrument, which is not convenient for carrying and on-site detection, which restricts the application and promotion of this technology. [0003] There is an irreconcilable contradiction between ...

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Problems solved by technology

[0002] The research and application of fluorescence immunoquantitative detection instruments have been able to realize the quantitative detection of test strips, but most of the instruments currently use a fixed optical mechanism and a motor to drive the kit to translate to complete the optical-mechanical scanning method to obtain C-line and T-line. The strength of the optical signal on the line, this method leads to the large size of the instrument, which is not easy to carry and on-site detection, which restricts the application and promotion of this technology

[0003] There is an irreconcilable contradiction between the volume reduction of the existing fluorescent immunoassay quantitative detection instruments and the improvement of quantitative detection accuracy

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