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Monochromatic fluorescence detection device

A fluorescent detection, monochromatic technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of volume reduction, quantitative detection accuracy, inconvenient portability and on-site detection, restrictions on application and promotion, etc., to achieve convenient on-site detection and small size , easy to carry effect

Pending Publication Date: 2018-03-16
NANJING INST OF ADVANCED LASER TECH
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Problems solved by technology

[0002] The research and application of fluorescence immunoquantitative detection instruments have been able to realize the quantitative detection of test strips, but most of the instruments currently use a fixed optical mechanism and a motor to drive the kit to translate to complete the optical-mechanical scanning method to obtain C-line and T-line. The strength of the optical signal on the line, this method leads to the large size of the instrument, which is not easy to carry and on-site detection, which restricts the application and promotion of this technology
[0003] There is an irreconcilable contradiction between the volume reduction of the existing fluorescent immunoassay quantitative detection instruments and the improvement of quantitative detection accuracy

Method used

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Embodiment Construction

[0012] The technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0013] Such as figure 1 As shown, the monochromatic fluorescence detection device of the present invention is applied to a portable fluorescence immunoassay analyzer, and includes a photodetector 1, an aperture diaphragm 2, a focusing lens 3, a first filter 4, a dichromatic mirror 5, and a parabolic reflector Mirror 6, second filter 7, collimating lens 8 and LED light source 9.

[0014] Photodetector 1, pinhole diaphragm 2, focusing lens 3, and first optical filter 4 are aligned in order to form a receiving optical path; the dichromatic mirror 5 and the optical axis of the receiving optical path form a 45° angle on the horizontal plane, and an off-axis paraboloid The reflector 6 forms a certain angle with the optical axis of the receiving optical path on the vertical plane, and reflects the optical axis downward to ensure tha...

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Abstract

The invention discloses a monochromatic fluorescence detection device. The monochromatic fluorescence detection device comprises a photoelectric detector, a micropore diaphragm, a focusing lens, a first optical filter, a dichroic mirror, a parabolic reflector, a second optical filter, a collimating lens and an LED light source. The photoelectric detector, the micropore diaphragm, the focusing lensand the first optical filter are sequentially arrayed in an optical axis coincided manner to form a receiving light path. Optical axes of the dichroic mirror and the receiving light path form a 45-degree angle horizontally. The second optical filter, the collimating lens and the LED light source behind the dichronic mirror are sequentially arrayed in an optical axis coincided manner to form an excitation light path. The detection device for temporal separation of C line and T line optical signals on the basis of an optical path conversion structure of a parabolic reflector micromotion mechanism is small in size, high in measurement precision, portable and convenient for onsite detection and can be applied to researching of fluorescence immunoassay quantitative detection instruments.

Description

technical field [0001] The invention relates to a monochrome fluorescence detection device applied to a portable fluorescence immunoassay analyzer, in particular to a monochrome fluorescence detection device which is based on a micro-movement mechanism of a parabolic mirror to switch the optical path of the stripe detection. Background technique [0002] The research and application of fluorescence immunoquantitative detection instruments have been able to realize the quantitative detection of test strips, but most of the instruments currently use a fixed optical mechanism and a motor to drive the kit to translate to complete the optical-mechanical scanning method to obtain C-line and T-line. The strength of the optical signal on the line, this method leads to the large size of the instrument, which is not convenient for carrying and on-site detection, which restricts the application and promotion of this technology. [0003] There is an irreconcilable contradiction between ...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N33/558
CPCG01N33/533G01N33/558
Inventor 王炜尚保华曲文静钱华泉谢涛童长华
Owner NANJING INST OF ADVANCED LASER TECH
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