Porcine NTF3 promoter region SNP as molecular marker for boar breeding traits and applications of porcine NTF3 promoter region SNP

A technique for molecular markers and boars, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial determination/inspection, etc.
CN107022604AActive Publication Date: 2017-08-08HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2017-08-08

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Abstract

The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to porcine NTF3 promoter region SNP as a molecular marker for the boar breeding traits and applications of the porcine NTF3 promoter region SNP. The molecular marker is cloned from the 5' flank promoter fragment of the porcine NTF3 gene, the nucleotide sequences of the molecular marker are shown as SEQ ID NO:1 and SEQ NO:2, allele mutation of A / G and G / A exists at the 92nd basic group site of the sequence shown as SEQ ID NO:1 and SEQ NO:2, and the allele mutation causes the polymorphism of MvaI-RFLP. The marker is utilized for carrying out correlation analysis on the boar breeding traits of large white pigs and duroc pigs. The breeding traits comprise the boar sperm motility and the sperm density trait. The invention further discloses a typing detection method of the marker, and therefore, a novel marker is provided for selecting the boar breeding traits. The marker can be used for evaluating the boar breeding traits and researching the genetic improvement on the breeding boar breeding performance.
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Description

technical field

[0001] The invention relates to the technical field of pig molecular marker screening, in particular to a pig NTF3 promoter region SNP as a boar reproductive traits molecular marker and its application. The present invention is related to the technical field of pig breeding and marker-assisted selection Background technique

[0002] Molecular marker-assisted selection (MAS, marker-assisted selection) is a new technology produced with the rapid development of modern molecular biology technology, which can quickly and accurately analyze the genetic composition of individuals at the molecular level, so as to realize the genotype Direct selection, molecular breeding. Molecular markers are directly expressed in the form of DNA, which can be detected in all tissues and developmental stages of organisms, and are not restricted by seasonal environments; polymorphisms are abundant and large in number; most of them are co-dominant, and can distinguish homozygous and h...

Claims

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