Application of pig SNP molecular marker in pig daily gain characters study and/or pig breeding

Abstract

The invention belongs to the fields of a molecular biotechnology and a molecule marker technology, and particularly relates to application of a pig SNP molecular marker in pig daily gain characters study and / or pig breeding. A pig SNP molecular marker loci is as shown in SEQ ID NO.1, and the 321st nucleic acid single-base located at a sequence segment is mutated and is named as g15588826C>T. An SNP molecular marker is mutated corresponding to the 15588826th nucleotide site C>T on an international pig reference genome 10.2 version chromosome 3. According to the invention, through optimizing a predominant allele of the SNP molecular marker, the genetic progress of daily gain characters of large white can be increased, and the breeding time of the daily gain characters of the large white can be reduced, so that the economic benefit of pig breeding is effectively improved.

Description

technical field

[0001] The invention belongs to the technical fields of molecular biotechnology and molecular markers, and particularly relates to the application of a porcine SNP molecular marker in the study of pig daily gain traits and / or pig breeding. Background technique

[0002] The daily gain trait is an important index to evaluate the growth performance of pigs, so it is the focus of the genetic improvement research of breeding pigs. In the past hundred years, the breeding goals of western pig breeds have often focused on increasing lean meat percentage and reducing backfat thickness, which has improved the genetic progress of daily gain to a certain extent, but there has been a lack of more direct breeding methods. Traditional breeding methods have little effect on traits that are phenotypically difficult to identify (such as daily weight gain), but molecular marker assisted selection (MAS) has broken this technical barrier. This method can be used to improve trait...

Examples

Embodiment 1

[0028] In the embodiment, a total of 689 purebred Large Whites were used; among them, the age of pigs when their body weight reached 30 kg was measured by the OSBORNE system and recorded as Day1, and the age of pigs when their body weight reached 100 kg was recorded as Day2. The formula for calculating daily weight gain is: 70 / (Day2-Day1). The above experiment was carried out in the Shahu Breeding Pig Farm of Guangdong Wenshi Food Group Co., Ltd. During the measurement period, 10 to 12 pigs were housed in each pen (each pig occupies an area of ​​2 square meters) with free access to food and water, and were fed according to the uniform Standard, uniform feeding ration.

[0029] (1) The method of extracting DNA from large white pig ear-like tissue refers to the phenol-chloroform method to extract whole-genome DNA. Use Nanodrop-ND1000 spectrophotometer to detect the quality and concentration of the DNA of the purebred Great White population. The ratio of A260 / 280 is 1.8-2.0, an...

Embodiment 2

[0036] (1) Amplification of the target fragment containing the SNP site significantly related to the performance of daylight weight gain. The target fragment is a 695bp nucleotide sequence in chromosome 3, and the upstream and downstream primers for sequence amplification are:

[0037] Upstream primer PCR-F: 5'-CAGGTAGGACACCTCCACT-3';

[0038] Downstream primer PCR-R: 5'-TGACAGCAGCACTAAATGAC-3'.

[0039] (2) PCR amplification system and condition setting

[0040] Configure 20uL system, including DNA sample 3.5μL, upstream primer 0.6μL, downstream primer 0.6μL, PCR mix 10mL, ddH 2 O 5.3 μL, PCR conditions were pre-denaturation at 98°C for 2 min, denaturation at 98°C for 10 s, annealing at 56.5°C for 15 s, extension at 72°C for 40 s, a total of 35 cycles, and the final extension at 72°C for 10 min.

[0041] (3) DNA sequence sequencing identification: Sequence sequencing was carried out in Shenzhen Huada Gene Technology Co., Ltd., and the positive and negative reactions of the ge...

Embodiment 3

[0045] Example 3 SNP site g.15588826C>T effect analysis of molecular markers

[0046] The invention provides a SNP marker capable of significantly increasing the daily gain of Large White pigs and their synthetic lines, and using the SNP for marker-assisted selection can greatly increase the daily gain breeding process of Large White pigs and their synthetic lines. All the CC-type individuals with molecular markers affecting pig daily gain traits of the present invention are bred into TT-type individuals, and the average daily gain of each pig can be increased by 88.7g, and a large-scale 10,000-head pig farm can increase pork within 70 days 62.09t, it can be seen that the excellent daily weight gain performance has great potential to provide income for the pig industry. Among individuals marked by this SNP, there is a significant difference in daily weight gain performance between CC individuals and TT individuals (P<0.01). By optimizing the dominant allele (T) of this SNP in ...

technical field

[0001] The invention belongs to the technical fields of molecular biotechnology and molecular markers, and particularly relates to the application of a porcine SNP molecular marker in the study of pig daily gain traits and / or pig breeding. Background technique

[0002] The daily gain trait is an important index to evaluate the growth performance of pigs, so it is the focus of the genetic improvement research of breeding pigs. In the past hundred years, the breeding goals of western pig breeds have often focused on increasing lean meat percentage and reducing backfat thickness, which has improved the genetic progress of daily gain to a certain extent, but there has been a lack of more direct breeding methods. Traditional breeding methods have little effect on traits that are phenotypically difficult to identify (such as daily weight gain), but molecular marker assisted selection (MAS) has broken this technical barrier. This method can be used to improve trait...

Examples

Embodiment 1

[0028] In the embodiment, a total of 689 purebred Large Whites were used; among them, the age of pigs when their body weight reached 30 kg was measured by the OSBORNE system and recorded as Day1, and the age of pigs when their body weight reached 100 kg was recorded as Day2. The formula for calculating daily weight gain is: 70 / (Day2-Day1). The above experiment was carried out in the Shahu Breeding Pig Farm of Guangdong Wenshi Food Group Co., Ltd. During the measurement period, 10 to 12 pigs were housed in each pen (each pig occupies an area of ​​2 square meters) with free access to food and water, and were fed according to the uniform Standard, uniform feeding ration.

[0029] (1) The method of extracting DNA from large white pig ear-like tissue refers to the phenol-chloroform method to extract whole-genome DNA. Use Nanodrop-ND1000 spectrophotometer to detect the quality and concentration of the DNA of the purebred Great White population. The ratio of A260 / 280 is 1.8-2.0, an...

Embodiment 2

[0036] (1) Amplification of the target fragment containing the SNP site significantly related to the performance of daylight weight gain. The target fragment is a 695bp nucleotide sequence in chromosome 3, and the upstream and downstream primers for sequence amplification are:

[0037] Upstream primer PCR-F: 5'-CAGGTAGGACACCTCCACT-3';

[0038] Downstream primer PCR-R: 5'-TGACAGCAGCACTAAATGAC-3'.

[0039] (2) PCR amplification system and condition setting

[0040] Configure 20uL system, including DNA sample 3.5μL, upstream primer 0.6μL, downstream primer 0.6μL, PCR mix 10mL, ddH 2 O 5.3 μL, PCR conditions were pre-denaturation at 98°C for 2 min, denaturation at 98°C for 10 s, annealing at 56.5°C for 15 s, extension at 72°C for 40 s, a total of 35 cycles, and the final extension at 72°C for 10 min.

[0041] (3) DNA sequence sequencing identification: Sequence sequencing was carried out in Shenzhen Huada Gene Technology Co., Ltd., and the positive and negative reactions of the ge...

Embodiment 3

[0045] Example 3 SNP site g.15588826C>T effect analysis of molecular markers

[0046] The invention provides a SNP marker capable of significantly increasing the daily gain of Large White pigs and their synthetic lines, and using the SNP for marker-assisted selection can greatly increase the daily gain breeding process of Large White pigs and their synthetic lines. All the CC-type individuals with molecular markers affecting pig daily gain traits of the present invention are bred into TT-type individuals, and the average daily gain of each pig can be increased by 88.7g, and a large-scale 10,000-head pig farm can increase pork within 70 days 62.09t, it can be seen that the excellent daily weight gain performance has great potential to provide income for the pig industry. Among individuals marked by this SNP, there is a significant difference in daily weight gain performance between CC individuals and TT individuals (P<0.01). By optimizing the dominant allele (T) of this SNP in ...

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