Molecular marking method for indicating and identifying litter size in pigs by MMP23 gene
A molecular marker and litter size technology, applied in the field of animal molecular genetics, can solve the problem of decreased expression and achieve good selection effect.
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Embodiment 1
[0026] Example 1: The acquisition of gene fragments and the establishment of polymorphism detection methods
[0027] Amplification of Partial DNA Sequence of Porcine MMP23 Gene
[0028] (1) Primer design
[0029] A foreign pig breed (Large White pig) and two Chinese pig breeds (Meishan pig and Qingping pig) were selected as test materials, and primers were designed according to the genome sequence of the pig MMP23 gene (GeneBank accession number: EU360790). The primer sequences are as follows:
[0030] Forward primer F: 5′-ACGCCGCTACACGCTGAC-3′
[0031] Reverse primer R: 5′-CGCTGTCGTCAAAGTGGATG-3′
[0032] Use this primer to carry out PCR amplification in three pig breeds (Large White pig, Meishan pig and Qingping pig) genomic DNA, PCR reaction system is 25 μ l, wherein template DNA is 50ng, dNTPs concentration is 200 μ mol / L, and each primer concentration is 0.4μmol / L, 2U Taq DNA polymerase (Biostar International, Canada), 2.5μl GC buffer, add deionized water to a total vo...
Embodiment 2
[0044] Example 2: Application of molecular markers in production traits
[0045] In order to determine whether the polymorphism of the MMP23 gene is related to the difference in pig phenotype, 40 Min pigs (207 litters) and 47 Landrace pigs (215 litters) from the Lanxi breeding pig farm in Heilongjiang Province were selected as test materials, and the data of each parity were sorted out. Farrowing traits records (2001-2006). The Sau3AI-RFLP method established in Example 1 was used for polymorphism detection, and the correlation between pig MMP23 gene Sau3AI-PFLP genotypes and pig litter size was analyzed. The SAS statistical software (SAS Institute Inc, Version 8.0) GLM program was used for single-marker analysis of variance, and the REG program was used to calculate the additive effect and dominant effect of the gene, and the significance test was carried out. The model used was:
[0046] Y ijkl =μ+G i +P j +Y k +S l ++e ijkl
[0047] Y ij is the trait phenotype value...
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