Molecular marking method for indicating and identifying litter size in pigs by MMP23 gene

A molecular marker and litter size technology, applied in the field of animal molecular genetics, can solve the problem of decreased expression and achieve good selection effect.

Inactive Publication Date: 2009-05-27
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology

Further studies showed that MMP23 was highly expressed in ovarian stromal cells and granulosa

Method used

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  • Molecular marking method for indicating and identifying litter size in pigs by MMP23 gene
  • Molecular marking method for indicating and identifying litter size in pigs by MMP23 gene
  • Molecular marking method for indicating and identifying litter size in pigs by MMP23 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: The acquisition of gene fragments and the establishment of polymorphism detection methods

[0027] Amplification of Partial DNA Sequence of Porcine MMP23 Gene

[0028] (1) Primer design

[0029] A foreign pig breed (Large White pig) and two Chinese pig breeds (Meishan pig and Qingping pig) were selected as test materials, and primers were designed according to the genome sequence of the pig MMP23 gene (GeneBank accession number: EU360790). The primer sequences are as follows:

[0030] Forward primer F: 5′-ACGCCGCTACACGCTGAC-3′

[0031] Reverse primer R: 5′-CGCTGTCGTCAAAGTGGATG-3′

[0032] Use this primer to carry out PCR amplification in three pig breeds (Large White pig, Meishan pig and Qingping pig) genomic DNA, PCR reaction system is 25 μ l, wherein template DNA is 50ng, dNTPs concentration is 200 μ mol / L, and each primer concentration is 0.4μmol / L, 2U Taq DNA polymerase (Biostar International, Canada), 2.5μl GC buffer, add deionized water to a total vo...

Embodiment 2

[0044] Example 2: Application of molecular markers in production traits

[0045] In order to determine whether the polymorphism of the MMP23 gene is related to the difference in pig phenotype, 40 Min pigs (207 litters) and 47 Landrace pigs (215 litters) from the Lanxi breeding pig farm in Heilongjiang Province were selected as test materials, and the data of each parity were sorted out. Farrowing traits records (2001-2006). The Sau3AI-RFLP method established in Example 1 was used for polymorphism detection, and the correlation between pig MMP23 gene Sau3AI-PFLP genotypes and pig litter size was analyzed. The SAS statistical software (SAS Institute Inc, Version 8.0) GLM program was used for single-marker analysis of variance, and the REG program was used to calculate the additive effect and dominant effect of the gene, and the significance test was carried out. The model used was:

[0046] Y ijkl =μ+G i +P j +Y k +S l ++e ijkl

[0047] Y ij is the trait phenotype value...

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Abstract

The invention aims to provide a detection method for searching the genetic mutation sites of porcine MMP23 and the genetic polymorphism, namely, the molecular marking method for speculating and identifying the litter size with MMP23. The invention provides the sequence of genomic nucleotides with a length of 482bp including the exons 2, 3 and 4 and the introns 2 and 3 of the porcine MMP23 gene of large white sows, Meishan pigs and Qingping pigs, and provides the single nucleotide polymorphism (SNP) of at two sites situating in the interior of the proliferated 482bp-long segment. The method provided by the invention can be used for developing diagnostic procedures or kits used for selecting the pigs carrying the advantageous alleles in breeding programs with better selective outcome.

Description

(1) Technical field [0001] The invention relates to animal molecular genetics, specifically a molecular marker method for predicting and identifying pig litter size with MMP23 gene. (2) Background technology [0002] For a long time, improving growth rate and lean meat rate, improving meat quality, increasing litter size, and enhancing disease resistance are the main goals of pig breeding. In the past few decades, with the improvement of breeding methods and the application of BLUP method, the inheritance of medium and high heritability traits such as pig growth speed, feed remuneration and lean meat percentage has progressed rapidly, and people have obtained great economic benefits from it. ; but the improvement in reproductive traits in western pig breeds has been minimal. According to reports, France has spent more than 30 years improving the litter size traits of pigs, but little progress has been made; Denmark has spent more than 50 years increasing the litter size by ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/12C12N15/11
Inventor 王希彪牛步月熊远著崔世泉
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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