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Molecular marker SV193 for distinguishing Chinese local pig breeds from Large White pigs and application technology of molecular marker SV193

A technology of SV193 and molecular markers, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems affecting the resistance of chickens to Marek's disease, excessive melanin deposition, etc., and achieve easy operation, accurate and reliable methods Effect

Active Publication Date: 2017-02-15
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the deletion-type structural variation of the chicken MHC class I gene will affect the resistance of chickens to Marek's disease; the 130Kb duplication in the chicken EDN3 gene leads to excessive deposition of melanin

Method used

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  • Molecular marker SV193 for distinguishing Chinese local pig breeds from Large White pigs and application technology of molecular marker SV193
  • Molecular marker SV193 for distinguishing Chinese local pig breeds from Large White pigs and application technology of molecular marker SV193
  • Molecular marker SV193 for distinguishing Chinese local pig breeds from Large White pigs and application technology of molecular marker SV193

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 The detection technique of the molecular marker SV193 for distinguishing pig breeds, the steps are as follows:

[0026] 1. Genomic DNA extraction

[0027] The present invention adopts the blood / cell / tissue genomic DNA extraction kit purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd. to extract genomic DNA from blood or tissue. The specific method is as follows:

[0028] 1) Processing materials

[0029] When the extraction material is blood, 200 μL of fresh, frozen or blood with various anticoagulants can be used directly, and buffer GA can be added to make up for less than 200 μL.

[0030] When the extracted material is animal tissue (the amount of spleen tissue should be less than 10 mg), crush it and process it into a cell suspension, then centrifuge at 10,000 rpm (11,200×g) for 1 min, pour off the supernatant, add 200 μL buffer GA, and shake until Completely levitate.

[0031] Note: To remove RNA, add 4 μL RNaseA solution, shake for 15 ...

Embodiment 2

[0050] Example 2 Correlation and detection application of SV193 genotype and pig breed type

[0051] According to the method described in Example 1, a total of 415 fragrant pigs from Congjiang County and Qingzhen, Guizhou, 46 radish pigs from Jiangkou County, Tongren City, 33 Cola pigs and 77 large white pigs from Bijie City, and Chongqing Forty-three Rongchang pigs in Rongchang County of the city were used as test materials, chr8:142093174-142093446 was used as the candidate SV193 deletion interval, and PCR technology was used to detect the distribution of the SV193 genotype in five populations, and the card in SPSS v20.0 software was used The square test was used to analyze the relationship between the differences of SV193 genotypes. The results show that (Table 1, figure 2 ) The genotype distribution frequency of SV193 in Large White pig and 4 local pig breeds was significantly different (P<0.001).

[0052] Table 1 The genotype and gene frequency of SV193

[0053]

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Abstract

The invention discloses a molecular marker SV193 for distinguishing Chinese local pig breeds from Large White pigs and an application technology of the molecular marker SV193. The molecular marker SV193 is characterized in that SV193 is located at chr8:142093174-142093446 of a pig reference genome Sscrofa 10.2, the deleted gene of SV193 is set to be D, and the normal undeleted gene is set to be A. With the application of the detection technology, the results that the Large White pigs mainly rely on the undeleted gene A while the Chinese local pig breeds mainly rely on the deleted gene D are obtained, and the Large White pigs can be distinguished from the Chinese local pig breeds by the application of the molecular marker SV193.

Description

technical field [0001] The invention relates to the field of livestock molecular biology detection, in particular to a molecular marker for distinguishing Chinese local pig breeds and large white pigs and its application technology. Background technique [0002] my country has abundant local pig genetic resources, which are an important part of the world's pig genetic resources. There is evidence that domestication from wild boars to domestic pigs was relatively independent in continental Asia and Europe, resulting in distinct characteristics of pig breeds in the two regions in terms of performance and body size. The large white pig, also known as the Great Yorkshire, originated in Europe and is one of the most widely distributed pig breeds in the world. Practical techniques for purity of local pig breeds. [0003] Insertions / deletions, duplications, inversions, translocations, and DNA copy number variations of DNA fragments larger than 1Kb that exist at the genome level a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 冉雪琴王嘉福徐倩牛熙
Owner GUIZHOU UNIV
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