Structural variation 177 (SV177) for distinguishing varieties of large white pigs and Chinese indigenous pigs, and detection technology of SV177

A technology of SV177 and structural variation, applied in the field of molecular biology detection, can solve problems such as abortion and stillbirth in cattle, and achieve the effect of simple operation, accurate and reliable method

Active Publication Date: 2017-02-22
GUIZHOU UNIV
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Problems solved by technology

For example, the repeated mutation of the first intron of the chicken SOX5 gene causes bean-shaped crown; t

Method used

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  • Structural variation 177 (SV177) for distinguishing varieties of large white pigs and Chinese indigenous pigs, and detection technology of SV177
  • Structural variation 177 (SV177) for distinguishing varieties of large white pigs and Chinese indigenous pigs, and detection technology of SV177
  • Structural variation 177 (SV177) for distinguishing varieties of large white pigs and Chinese indigenous pigs, and detection technology of SV177

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 The detection technique for distinguishing the structural variation SV177 of pig breeds, the steps are as follows:

[0026] 1. Genomic DNA extraction

[0027] The present invention adopts the blood / cell / tissue genomic DNA extraction kit purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd. to extract genomic DNA from blood or tissue. The specific method is as follows:

[0028] 1) Processing materials

[0029] When the extraction material is blood, 200 μL of fresh, frozen or blood with various anticoagulants can be used directly, and buffer GA can be added to make up for less than 200 μL.

[0030] When the extracted material is animal tissue (the amount of spleen tissue should be less than 10 mg), crush it and process it into a cell suspension, then centrifuge at 10,000 rpm (11,200×g) for 1 min, pour off the supernatant, add 200 μL buffer GA, and shake until Completely levitate.

[0031] Note: To remove RNA, add 4 μL RNaseA solution, shake for...

Embodiment 2

[0050] Example 2 Detection Application of Structural Variation SV177 Genotype

[0051] According to the method described in Example 1, a total of 299 fragrant pigs from Congjiang County and Qingzhen, Guizhou, 41 Jiangkou radish pigs from Jiangkou County, Tongren City, 34 Kele pigs and 52 Large White pigs from Bijie City were collected. And 44 Rongchang pigs from Rongchang County, Chongqing City were used as test materials, chrX: 100561156-100562159 was used as candidate SV177, the distribution of the SV177 genotype in the five populations was detected by PCR technology, and the card in SPSS v20.0 software was used. The square test was used to analyze the relationship between the differences of SV177 genotypes. The results show that (Table 1, figure 2 ) Large White pig has three genotypes, while the other four local pig breeds have at most two homozygous genotypes (AA, DD) and no heterozygous genotype (DA); Large White pig and four local pig breeds SV177 genotype The differe...

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Abstract

The invention discloses a structural variation 177 (SV177) for distinguishing varieties of large white pigs and Chinese indigenous pigs. The SV177 is characterized in that the SV177 is positioned at chrX: 100561156-100562159 of a pig reference genome Sscrofa 10.2, missing genes of the SV177 are determined as D, and normal genes which are not missed are determined as A. When a detection technology provided by the invention is used for detecting the SV177 genotype of a sample, the large white pigs have three genotypes and give priority to the genes A which are not missed, and the Chinese indigenous pigs are free from a heterozygous genotype (DA) and give priority to the missing genes D; therefore, the SV177 can be used for distinguishing the varieties of the large white pigs and the Chinese indigenous pigs as a molecular marker.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to a structural variation for distinguishing large white pigs from Chinese local pig breeds and a detection technology thereof. [0002] technical background [0003] The ancestors of all dynasties in our country all used the breeding idea of ​​breeding purebreds as live pig varieties. Due to the diversity and complexity of the regional environment and climate, the working people in different regions have unremittingly carried out the selection and breeding of local pig breeds. After a long period of time, they have cultivated local pig breeds suitable for the local environment, climate and other natural conditions, forming a There are rich and diverse local pig breed resources in my country. For example, the Xiang pig, Kele pig, Jiangkou radish pig and other pig breeds in Guizhou are all formed through long-term artificial breeding. They have the characteristics of early s...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156C12Q2600/172
Inventor 冉雪琴王嘉福徐倩牛熙
Owner GUIZHOU UNIV
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