Genetic marker associated with pig meat quality characters and carcass characters

A carcass trait and genetic marker technology, applied in the field of genetic markers, can solve the problems of low breeding efficiency and slow progress of breeding work.

Inactive Publication Date: 2016-07-13
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Traditional breeding mainly relies on phenotypic selection, and various factors such as environmental conditions, gene interaction, and genotype-e

Method used

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  • Genetic marker associated with pig meat quality characters and carcass characters
  • Genetic marker associated with pig meat quality characters and carcass characters
  • Genetic marker associated with pig meat quality characters and carcass characters

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Obtaining of pig BMP-3b gene DNA fragment and establishment of SNP detection method

[0029] In the experiment, two types of pig breeds, the foreign blood-related pig breed "Dabai pig" and the Chinese local blood-related pig breed "Meishan pig" (the biological material came from the boutique pig farm of Huazhong Agricultural University, are commonly used) were selected as materials. According to the pig BMP-3b gene genome Sequence (accession number GeneID: 100519926) designed the following primer pair, the specific sequence is as follows:

[0030] Forward primer F: 5'-TCACTGAAAATAGCAAAGGT-3',

[0031] Reverse primer R: 5'-ACTGGGCAGACAGGAGAAGC-3';

[0032] PCR amplification was carried out in the genomic DNA of "Large White pig" and "Meishan pig" with the above primers.

[0033] The PCR reaction system is shown in Table 1.

[0034] Table 1 PCR reaction system

[0035]

[0036] The PCR reaction conditions are shown in Table 2.

[0037] Table 2PCR rea...

Embodiment 2

[0041] Example 2: Association analysis and application of genetic markers of the present invention in pork quality traits and carcass traits

[0042] In order to determine whether the SNPs in the CDS region of pig BMP-3b gene are related to pig phenotype differences, 399 large white pigs × Meishan pig F 2 Generation resource population (Liu et al.Association of MYF5 and MYOD1 gene polymorphisms and meat quality traits in LargeWhite×MeishanF2pigpopulations.BiochemGenet.2008,46:720-732) was used as the experimental material, polymorphism detection was carried out by conventional AlwNI-RFLP method, and the difference between different genotypes in the CDS region of pig BMP-3b gene and pork Correlations between quality traits and carcass traits. SAS statistical software GLM program was used to conduct single-marker analysis of variance, and the model was as follows:

[0043] Meat quality traits analysis model: Y ij =μ+G i +S j +Y l +b ijkl +e ijkl

[0044] Carcass trait an...

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Abstract

The invention belongs to the technical field of pig genetic marker preparation and particularly relates to a genetic marker associated with pig meat quality and carcass characters, wherein the marker is obtained by screening the pig BMP-3b gene, and the sequence is shown by SEQ ID NO:1 and figure 4; and A/G base mutation happens at the 425th-site base of the sequence and causes AlwNI-RFLP polymorphism. The screening method comprises the following steps: extracting genome DNA from pig blood; designing a primer; performing PCR amplification; cloning the PCR product and sequencing; comparing and analyzing the sequence; performing SNP detection; and analyzing the correlation between the marker and the meat quality characters. The invention discloses an SNP typing detection technology for the CDS area of the pig BMP-3b gene, and significant correlation is discovered between the A/G mutation site and the meat quality characters such as marbling, color value and intramuscular fat content and between the A/G mutation site and the carcass characters such as average back-fat thickness, eye muscle height and leaf fat. The invention provides a new marker resource for pig marker assisted selection.

Description

technical field [0001] The invention belongs to the technical field of pig molecular marker-assisted selection, and in particular relates to a genetic marker associated with pork quality and carcass traits. The genetic marker is isolated from the CDS region fragment of the BMP-3b gene, and the present invention also includes the application of the genetic marker in pig-assisted selection association analysis. Background technique [0002] Traditional breeding mainly relies on phenotypic selection, and many factors such as environmental conditions, gene interaction, and genotype-environment interaction will affect the efficiency of phenotypic selection, making the efficiency of traditional breeding low and the progress of breeding work slow. Breeders have been exploring the use of genetic markers to improve the selection efficiency and breeding predictability in long-term breeding practice. Genetic markers refer to material markers that can be used to distinguish biological ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 左波吴欣玉纪攀龙徐珍熊远著
Owner HUAZHONG AGRI UNIV
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