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Molecular marker influencing intramuscular fat character of pig and application

An intramuscular fat and molecular marker technology, which can be used in the fields of molecular biotechnology and genetic breeding to solve problems such as time-consuming and labor-intensive

Active Publication Date: 2016-06-01
SOUTH CHINA AGRI UNIV
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Problems solved by technology

The development of the pig industry is determined by the economic traits of breeding pigs, and economic traits are genetically improved by breeding methods such as phenotypic selection, which is time-consuming and labor-intensive; therefore, finding the main effect that can improve production performance and reduce production costs Gene molecular markers, and genetic improvement methods based on molecular markers have become one of the important work directions for pig breeders

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  • Molecular marker influencing intramuscular fat character of pig and application
  • Molecular marker influencing intramuscular fat character of pig and application
  • Molecular marker influencing intramuscular fat character of pig and application

Examples

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Embodiment 1

[0023] Example 1 specifically explains the invention process of molecular markers in the present invention

[0024] 1. Porcine whole genome 60KSNP judgment type

[0025] A small piece of ear sample was collected from each individual of 972 Duroc boars selected from the above-mentioned resource groups. The whole genome DNA was extracted by using the standard phenol-chloroform method, and the Nanodrop-1000 nucleic acid protein analyzer was used to detect these DNA quality, and then uniformly dilute the qualified DNA samples to 50ng / μl, and send them to Beijing Yimeitongde Co., Ltd. on the IlluminaBeadstration platform for pig whole genome 60KSNP chip (Illumina, USA) genotype determination according to the company's standard process. Use the checkmarker in the R language GenABEL package to perform quality control on the 60K chip scanning typing data of all samples, and eliminate the detected individual rate below 95%, the family Mendelian error rate is greater than 0.1, the minimum al...

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Abstract

The invention discloses a molecular marker influencing the intramuscular fat character of a pig and application, and belongs to the field of molecular biological technologies and genetic breeding. The molecular marker is located at the 23147683rd locus of the nucleotide sequence of the eighth chromosome of the pig, the nucleotide sequence of the molecular marker is shown in SEQ IDNO:1, and the G>A allelic mutant is located at the 400th nucleotide of the genomic sequence of the pig. The new marker is provided for the genetic improvement of the pig, and the genetic breeding work of the pig of the good strain is facilitated.

Description

Technical field [0001] The invention belongs to the fields of molecular biotechnology and genetic breeding, and particularly relates to a molecular marker that affects pig intramuscular fat traits and its application. Background technique [0002] With the improvement of living standards, people pay more and more attention to their own health. Delicious, nutritious and green meat products have become a new goal pursued by people. Pork is a major and universal source of animal fat for the human body. Pork quality requirements are getting higher and higher. The research focus of the development of the pig industry has always been to breed high-quality, high-yield and high-quality pigs. The development of the pig industry is determined by the economic traits of breeding pigs, and the economic traits are genetically improved by breeding methods such as phenotypic selection, which is time-consuming and labor-intensive; therefore, find the main effects that can improve production perf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 吴珍芳杨杰杨林雪蔡更元刘德武李紫聪郑恩琴顾婷
Owner SOUTH CHINA AGRI UNIV
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