A kind of whole pig origin African swine fever virus monoclonal antibody and preparation method thereof

Abstract

The invention belongs to the field of biotechnology, and in particular relates to a monoclonal antibody of whole swine origin African swine fever virus and its preparation method and application. The monoclonal antibody comprises a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is as SEQ ID NO: 1 or SEQ ID NO: 5 As shown, the amino acid sequence of the light chain variable region is shown in SEQ ID NO:2 or SEQ ID NO:6. The whole pig-derived monoclonal antibody specific for African swine fever virus of the present invention retains the natural pairing of light chain and heavy chain variable regions, and has good gene diversity, whole pig origin, good antibody affinity, strong specificity, etc. Advantages, high-efficiency expression can be performed in eukaryotic expression system. The invention provides new raw materials for the development of African swine fever virus diagnostic kits, and also provides new technical support for the development of preventive and therapeutic drugs for whole pig-derived monoclonal antibodies.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a monoclonal antibody of whole swine origin African swine fever virus and a method for preparing the monoclonal antibody by high-throughput single B cell PCR technology. Background technique [0002] African swine fever (ASF) is an acute and highly contagious disease of pigs caused by African swine fever virus (ASFV), with a short course and a fatality rate of up to 100%. The World Health Organization (OIE) lists it as a legally notifiable animal disease, and my country lists it as a first-class animal disease. ASF was first discovered in Kenya in 1921. It was introduced into my country in August 2018, and then quickly spread to many provinces and cities in my country, causing huge economic losses to the development of my country's pig industry. So far, there is no effective commercial vaccine for the prevention and control of ASF. In the short term, early diagnosis and ...

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Problems solved by technology

[0004] The invention patents with publication numbers CN112592401A and CN112500478A respectively disclose a ScFv antibody VH-VLλ11 and VH-VLλ6 against African swine fever virus, and their preparation methods, although VH-VLλ11 and VH-VLλ6 are antibodies to African swine fever virus , but the antibodies are all single-chain antibodies, single-chain antibodies only include the heavy chain variable region and the light chain variable region, not including the constant region

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