Full-swine-origin African swine fever virus monoclonal antibody and preparation method thereof

Abstract

The invention belongs to the technical field of biology, and particularly relates to a full-swine-origin African swine fever virus monoclonal antibody as well as a preparation method and application thereof. The monoclonal antibody comprises heavy chain constant regions, heavy chain variable regions, light chain constant regions and light chain variable regions, the amino acid sequences of the heavy chain variable regions are as shown in SEQ ID NO: 1 or SEQ ID NO: 5, and the amino acid sequences of the light chain variable regions are as shown in SEQ ID NO: 2 or SEQ ID NO: 6. The full-swine-origin monoclonal antibody specifically aiming at the African swine fever virus retains natural pairing of light chain and heavy chain variable regions, has the advantages of good gene diversity, full swine origin, good antibody affinity, strong specificity and the like, and can be efficiently expressed in a eukaryotic expression system. According to the invention, a novel raw material is provided for developing a diagnostic kit for African swine fever virus and a new technical support is also provided for developing a preventive and therapeutic drug of a full-swine-origin monoclonal antibody.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and more particularly to a whole pig source African swine fever virus monoclonal antibody and a method of preparing the monoclonal antibody using a single B cell PCR technology using high throughput. Background technique [0002] African Swine Fever (ASF) is an acute, highly exposed to infectious disease caused by African Swine Fevervirus, ASFV, and is short, and the disease rate is up to 100%. The World Health Organization (OIE) is listed as a legal reported animal disease, and my country is listed as a class of animal diseases. ASF was first found in Kenya in 1921. In August 2018, I was introduced to my country. Subsequently spread to many provinces and cities in my country, and the development of pig industry in China has caused huge economic losses. So far, there is no effective commercial vaccine to prevent ASF, relying on early diagnosis and regional prevention and management management in t...

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Problems solved by technology

[0004] The invention patents with publication numbers CN112592401A and CN112500478A respectively disclose a ScFv antibody VH-VLλ11 and VH-VLλ6 against African swine fever virus, and their preparation methods, although VH-VLλ11 and VH-VLλ6 are antibodies to African swine fever virus , but the antibodies are all single-chain antibodies, single-chain antibodies only include the heavy chain variable region and the light chain variable region, not including the constant region

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