Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Full-swine-origin African swine fever virus monoclonal antibody and preparation method thereof

An African swine fever virus and monoclonal antibody technology, which is applied in the biological field to achieve the effects of good gene diversity, reduced production costs and strong specificity

Active Publication Date: 2021-08-20
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention patents with publication numbers CN112592401A and CN112500478A respectively disclose a ScFv antibody VH-VLλ11 and VH-VLλ6 against African swine fever virus, and their preparation methods, although VH-VLλ11 and VH-VLλ6 are antibodies to African swine fever virus , but the antibodies are all single-chain antibodies, single-chain antibodies only include the heavy chain variable region and the light chain variable region, not including the constant region

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Full-swine-origin African swine fever virus monoclonal antibody and preparation method thereof
  • Full-swine-origin African swine fever virus monoclonal antibody and preparation method thereof
  • Full-swine-origin African swine fever virus monoclonal antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0041] The present invention will be described in more detail below to facilitate understanding of the technical solutions of the present invention, but are not intended to limit the scope of the invention.

[0042] Sample acquisition and PBMC separation

[0043] The cervic vein is collected by African swine fever immunized pig (5 months old, from a pig farm from Xinxiang, Henan) for PBMC separation. The method of operation is made by the method of separation kit (Tianjin Yuli / TBD, LTS1110) instructions in pig.

[0044] 2. Separation and screening of target B lymphocytes

[0045] ASFV antigen-specific pig B lymphocytes were selected using ASFV and antigen IgG double markers. After ASFV is inactivated, use biotin (thermo scientific) TM EZ-LINK TM Sulfo-NHS-LC-Biotinylation Kit, Thermofish, 21435) is labeled.

[0046] 2.1ASFV positive B lymphocytes

[0047] The separated lymphocytes were counted, and the cells were diluted to 1 × 10 with PBS. 7I / ml. 200 μl of cells were added, 2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biology, and particularly relates to a full-swine-origin African swine fever virus monoclonal antibody as well as a preparation method and application thereof. The monoclonal antibody comprises heavy chain constant regions, heavy chain variable regions, light chain constant regions and light chain variable regions, the amino acid sequences of the heavy chain variable regions are as shown in SEQ ID NO: 1 or SEQ ID NO: 5, and the amino acid sequences of the light chain variable regions are as shown in SEQ ID NO: 2 or SEQ ID NO: 6. The full-swine-origin monoclonal antibody specifically aiming at the African swine fever virus retains natural pairing of light chain and heavy chain variable regions, has the advantages of good gene diversity, full swine origin, good antibody affinity, strong specificity and the like, and can be efficiently expressed in a eukaryotic expression system. According to the invention, a novel raw material is provided for developing a diagnostic kit for African swine fever virus and a new technical support is also provided for developing a preventive and therapeutic drug of a full-swine-origin monoclonal antibody.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and more particularly to a whole pig source African swine fever virus monoclonal antibody and a method of preparing the monoclonal antibody using a single B cell PCR technology using high throughput. Background technique [0002] African Swine Fever (ASF) is an acute, highly exposed to infectious disease caused by African Swine Fevervirus, ASFV, and is short, and the disease rate is up to 100%. The World Health Organization (OIE) is listed as a legal reported animal disease, and my country is listed as a class of animal diseases. ASF was first found in Kenya in 1921. In August 2018, I was introduced to my country. Subsequently spread to many provinces and cities in my country, and the development of pig industry in China has caused huge economic losses. So far, there is no effective commercial vaccine to prevent ASF, relying on early diagnosis and regional prevention and management management in t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C12N15/13C12N15/85G01N33/577G01N33/569A61K39/42A61P31/20
CPCC07K16/081C12N15/85G01N33/577G01N33/56983A61P31/20C07K2317/52C07K2317/56G01N2333/01
Inventor 郑海学曹丽艳田宏王琦孔祥雨宋锐石正旺
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com