Method for matching and breeding jian carps

Abstract

The invention relates to a method for matching and breeding jian carps. The method comprises the following steps of: sampling blood or fin rays of a jian carp breeding population to extract genome DNA; detecting the genotype of SNPs (Single Nucleotide Polymorphisms) by a PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method constructed by taking the extracted genomeDNA as a template according six SNP sites related to jian carp weight increment; carrying out genotype determination by using a sepharose gel electrophoresis enzyme slitting strip; selecting individuals containing more than four genotypes with rapid weight increment as candidate parents; carrying out digital treatment on the candidate parents by using the PCR-RFLP sepharose gel electrophoresis enzyme slitting strip; analyzing by using genetic analysis software to obtain genetic distances between every two individuals; constructing an evolutionary tree; and selecting an individual which has a larger genetic distance and better gonad development and can enrich more than four genotypes with rapid weight increment. The method is helpful to improving the production performance of jian carps, avoiding inbreeding and accelerating the breeding process of jian carps.

Description

technical field

[0001] The invention belongs to the field of assisted breeding of fish molecular markers, and relates to a method for establishing group breeding of carps. Background technique

[0002] Jian carp (Cyprinus carplo vat.jian) has the advantages of fast growth, good body shape, blue-gray color, good meat quality and taste, high feed conversion rate, strong adaptability and stress resistance, etc. It is bred through family selection, multi-line hybridization and female A carp variety with stable genetic traits successfully bred by the comprehensive breeding technology combined with nuclear development. Although Jian carp has relatively comprehensive and excellent traits, due to the expansion of breeding areas and non-standard breeding techniques, Jian carp has inbreeding depression phenomena, such as slowed growth, decreased disease resistance, and smaller sexually mature individuals. Molecular marker-assisted selection is to use genetic marker information, indiv...

Examples

Embodiment 1

[0021] Example 1: Jian carp candidate parent selection and evolutionary tree construction

[0022] There are 937 Jian carp breeding groups (486 males and 451 females), which were bred for the Yixing Fishery of the Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences. 0.5 mL of blood was collected from the tail vein of each fish, and 30 μL of blood cells were taken to extract genomic DNA. Using the extracted genomic DNA as a template, the PCR-RFLP method was used to detect the genotype of 6 SNP loci related to the weight gain of Jian carp for each individual. The total volume of PCR is 12.5 μL, containing 50-100 ng of genomic DNA, and the remaining components are added according to the Taq enzyme instructions. The PCR reaction conditions are: 94°C for 2min; 94°C for 30s, annealing for 40s, 72°C for 1min, 30 cycles; 72°C for extension 8 minutes, store at 4°C. . At the end of the reaction, take 3-5 μL of the PCR solution to detect its concentration a...

Embodiment 2

[0024] The selection of candidate parents of Jian carp and the construction of the evolutionary tree are the same as in Example 1, from which the offspring with a genetic distance of 0.3 or more, on different branches of the evolutionary tree, mature gonads, and mating groups are enriched with 5 to 6 offspring with higher weight gain. Fast genotype females, males and 10 tails were bred one-to-one. The test results of their offspring showed that the average genetic distance was 0.3978, and 5-6 genotypes with faster weight gain were enriched.

Embodiment 3

[0026] The selection of candidate parents of Jian carp and the construction of phylogenetic tree are the same as in Example 1, from which the female fish 40, 40, 40, 30 male fish were used for group breeding (experimental group), and at the same time, 10 female fish and 10 male fish were randomly selected from non-candidate parents for group breeding (control group). Under the same culture conditions, the experimental group grew on average 13.52% faster than the control group.

technical field

[0001] The invention belongs to the field of assisted breeding of fish molecular markers, and relates to a method for establishing group breeding of carps. Background technique

[0002] Jian carp (Cyprinus carplo vat.jian) has the advantages of fast growth, good body shape, blue-gray color, good meat quality and taste, high feed conversion rate, strong adaptability and stress resistance, etc. It is bred through family selection, multi-line hybridization and female A carp variety with stable genetic traits successfully bred by the comprehensive breeding technology combined with nuclear development. Although Jian carp has relatively comprehensive and excellent traits, due to the expansion of breeding areas and non-standard breeding techniques, Jian carp has inbreeding depression phenomena, such as slowed growth, decreased disease resistance, and smaller sexually mature individuals. Molecular marker-assisted selection is to use genetic marker information, indiv...

Examples

Embodiment 1

[0021] Example 1: Jian carp candidate parent selection and evolutionary tree construction

[0022] There are 937 Jian carp breeding groups (486 males and 451 females), which were bred for the Yixing Fishery of the Freshwater Fisheries Research Center of the Chinese Academy of Fishery Sciences. 0.5 mL of blood was collected from the tail vein of each fish, and 30 μL of blood cells were taken to extract genomic DNA. Using the extracted genomic DNA as a template, the PCR-RFLP method was used to detect the genotype of 6 SNP loci related to the weight gain of Jian carp for each individual. The total volume of PCR is 12.5 μL, containing 50-100 ng of genomic DNA, and the remaining components are added according to the Taq enzyme instructions. The PCR reaction conditions are: 94°C for 2min; 94°C for 30s, annealing for 40s, 72°C for 1min, 30 cycles; 72°C for extension 8 minutes, store at 4°C. . At the end of the reaction, take 3-5 μL of the PCR solution to detect its concentration a...

Embodiment 2

[0024] The selection of candidate parents of Jian carp and the construction of the evolutionary tree are the same as in Example 1, from which the offspring with a genetic distance of 0.3 or more, on different branches of the evolutionary tree, mature gonads, and mating groups are enriched with 5 to 6 offspring with higher weight gain. Fast genotype females, males and 10 tails were bred one-to-one. The test results of their offspring showed that the average genetic distance was 0.3978, and 5-6 genotypes with faster weight gain were enriched.

Embodiment 3

[0026] The selection of candidate parents of Jian carp and the construction of phylogenetic tree are the same as in Example 1, from which the female fish 40, 40, 40, 30 male fish were used for group breeding (experimental group), and at the same time, 10 female fish and 10 male fish were randomly selected from non-candidate parents for group breeding (control group). Under the same culture conditions, the experimental group grew on average 13.52% faster than the control group.

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