Microsatellite molecular marker-specific primer of litopenaeus vannamei and its application in genetic diversity analysis

A technology of genetic diversity and molecular markers, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve the effect of reducing development costs, improving efficiency and simple operation

Active Publication Date: 2019-10-18
天津市水产研究所
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  • Abstract
  • Description
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Problems solved by technology

At present, the published effective microsatellite markers of Litopenaeus vannamei are far from meeting the needs of the above research

Method used

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  • Microsatellite molecular marker-specific primer of litopenaeus vannamei and its application in genetic diversity analysis
  • Microsatellite molecular marker-specific primer of litopenaeus vannamei and its application in genetic diversity analysis
  • Microsatellite molecular marker-specific primer of litopenaeus vannamei and its application in genetic diversity analysis

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Embodiment Construction

[0061] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0062] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0063] A specific primer for microsatellite molecular markers of Litopenaeus vannamei, respectively named LavTF001, LavTF003, LavTF005, LavTF006, LavTF007, LavTF015, LavTF018, LavTF022, LavTF025, LavTF028 and LavTF029, including 22 specific primer sequences, specifically:

[0064] LvaTF001: F: its nucleotide sequence is SEQ ID NO: 1, R: its nucleotide sequence is SEQ ID NO: 2;

[0065] LvaTF003: F: its nucleotide sequence is SEQ ID NO: 3, R: its nucleotide sequence is SEQ ID NO: 4;

[0066] LvaTF005: F: its nucleotid...

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Abstract

The invention relates to a microsatellite molecular marker-specific primer of litopenaeus vannamei, which can be named as LavTF001, LavTF003, LavTF005, LavTF006, LavTF007, LavTF015, LavTF018, LavTF022, LavTF025, LavTF028 and LavTF029, and comprises 22 specific primer sequences. The microsatellite marker is stable and reliable, and can be used for genetic diversity analysis, germplasm identification and evaluation, functional gene mapping and molecular breeding research of litopenaeus vannamei population.

Description

technical field [0001] The invention belongs to the technical field of molecular biology DNA molecular markers, in particular to a specific primer for microsatellite molecular markers of Litopenaeus vannamei and its application in genetic diversity analysis. Background technique [0002] Litopenaeus vannamei, also known as Penaeus vannamei, Penaeus vannamei, and Penaeus vannamei, belongs to the family Penaeidae and the genus Penaeus, and is native to the warm waters of the Pacific coast of Central and South America. Because of its wide range of adaptability to salinity, fast growth, strong disease resistance, and delicious meat, it has become the most important cultured prawn species in China and the world. However, with the deterioration of high-density farming and ecological environment, frequent outbreaks of prawn diseases have brought huge economic losses to the prawn industry in Vietnam, China, Malaysia and many other countries, seriously restricting the further develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156Y02A40/81
Inventor 李春艳刘克明刘肖莲马林白晓慧姜巨峰蔡超孟一耕王娜
Owner 天津市水产研究所
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