Microsatellite molecular marker-specific primer of litopenaeus vannamei and its application in genetic diversity analysis
A technology of genetic diversity and molecular markers, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve the effect of reducing development costs, improving efficiency and simple operation
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[0061] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.
[0062] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.
[0063] A specific primer for microsatellite molecular markers of Litopenaeus vannamei, respectively named LavTF001, LavTF003, LavTF005, LavTF006, LavTF007, LavTF015, LavTF018, LavTF022, LavTF025, LavTF028 and LavTF029, including 22 specific primer sequences, specifically:
[0064] LvaTF001: F: its nucleotide sequence is SEQ ID NO: 1, R: its nucleotide sequence is SEQ ID NO: 2;
[0065] LvaTF003: F: its nucleotide sequence is SEQ ID NO: 3, R: its nucleotide sequence is SEQ ID NO: 4;
[0066] LvaTF005: F: its nucleotid...
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