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Method for establishing super-male yellow cartfish breeding population by copulating super-male fish with physiology spawner

A technology for yellow catfish and female fish, which is applied in fish farming, application, climate change adaptation, etc., can solve the problem of unsatisfactory survival rate of gynogenetic fish, large-scale production that needs to be improved, and breeding cycle that needs to be shortened, etc. problem, to achieve the effect of saving gynogenesis process, reducing damage and improving genetic polysexuality

Inactive Publication Date: 2009-03-25
WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
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Problems solved by technology

But at the same time, there are still the following problems: the cold shock treatment of gynogenesis will damage the genetic material; the survival rate of the two-belief gynogenetic fish is not ideal; the scale production needs to be improved; the breeding cycle needs to be shortened

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  • Method for establishing super-male yellow cartfish breeding population by copulating super-male fish with physiology spawner

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Embodiment Construction

[0035] Applicants consider the procedures illustrated with reference to the drawings to be the preferred mode of carrying out the invention. The details of these processes are as follows:

[0036] 1. Artificial oxytocin and artificial insemination According to the needs of experiments or industrial production, take a certain number of ordinary orpiment catfish with no injury on the body surface, swollen abdomen, and softness, and inject once at the base of the pectoral fin of each fish. The oxytocic agent and dosage used As follows: HCG900IU+LRH-Az6 μg / tail, oxytocic agents HCG and LRH-Az are both products of Hangzhou Animal Medicine Factory and Xiaoshan First Veterinary Medicine Manufacturing Co., Ltd. Put the fish in water at 26°C, and after about 26 hours, the mature eggs can be squeezed out, and then placed on an ice bath for storage at low temperature for later use. At the same time, select male fish with reddish genital process to inject half of the same oxytocic agent,...

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Abstract

Disclosed is a method for super-male fish to mate physiological spawner to build super-male yellow catfish breeding groups, belonging to the fish physiological genetics; the method belongs to the technical field of controlling the sex of fish through artificial sex reversal, screening, test cross and group expanding. The technical problem to be solved is that issues are the super-male fish mates the sex-reversed physiological spawner; the gynogenesis process is eliminated; through the mating between the sex-reversed physiological spawner and the super-male fish or male fish, and the combination of the sex ratio analysis and the test cross technology, the breeding time is shortened. The invention adopts the following technical proposal: artificial spawning inducement and fertilization; sex reversal, namely, the male fish is sex-reversed into physiological spawner; test cross sex reversal, namely, the physiological spawner and the super-male or ordinary male fish are test-crossed, and the physiological spawner and the super-male fish are selected, and the test cross sex reversal comprises the artificial spawning inducement and fertilization; sex ratio analysis, namely, the sex of the candidate fish is confirmed; offspring determination, screening and identification; group expanding, that is, the breeding groups of the super-male fish and the super-female physiological spawner are expanded; and the breeding production of all-male fish, namely, ordinary spawner mates the super-male fish to produce the all-male fish. The method is used for the breeding and mass production of super-male yellow catfish breeding groups.

Description

technical field [0001] The method of mating super male fish with physiological female fish to establish breeding population of realgar catfish belongs to a method of fish physiological genetic reproduction, and is in the technical field of fish sex control by techniques such as artificial sex reversal, screening, test cross and population expansion. Background technique [0002] There are several methods for producing parthenogenetic males (Aquaculture2001, 197: 283-301), such as artificial selection, direct androgen-induced sex reversal, androgenesis, interspecific hybridization, artificially induced triploids, and genetic methods. However, manual selection is labor-intensive and cannot accurately distinguish between male and female fish. Direct induction of sex reversal with sex hormones has the risk of residual sex hormones, which has long been banned in many countries. Androgenic development has serious technical obstacles such as low induction rate, low survival rate, ...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘汉勤陈宏溪徐江侯昌春
Owner WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
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