SSCP (single strand conformation polymorphism) detecting method for animal liver bacterial toxin contamination

Abstract

The invention discloses an SSCP (single strand conformation polymorphism) detecting method for animal liver bacterial toxin contamination, relates to the biological technical field and particularly relates to a detecting method for animal liver bacterial toxin contamination. According to the method, an SSCP technology is utilized to detect gene mutation of animal liver mitochondria contaminated by bacterial toxin, so that the technology is applied to evaluating and identifying animal food safety. Main steps comprise extracting host liver mitochondria DNA (deoxyribonucleic acid), amplifying the liver mitochondria tRNACys-Tyr gene segment by applying PCR(polymerase chain reaction)-SSCP technology, detecting a stripe with abnormal swimming motility by polyacrylamide gel electrophoresis, sequencing after purifying the stripe, and indicating that the animal food is contaminated by the bacterial toxin if the tRNACys -Tyr gene segment is inserted with insertion mutation of a basic group T at the 5238th and 5239th basic groups. The SSCP detecting method disclosed by the invention can be used for solving the defects of the conventional detecting method, and is quick, accurate, specific, sensitive, good in repeatability, low in detecting cost and easy for popularization and application.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection method for animal liver bacterial toxin contamination. Background technique [0002] Bacterial infection and the abuse of antibiotics in the process of large-scale feeding of food animals often lead to bacterial infection and bacterial toxin pollution in animals. The liver is the main detoxification organ of animals and is easily contaminated by bacterial toxins. Chinese people have the habit of eating animal offal. Therefore, it is particularly necessary to detect whether animal liver is contaminated by bacterial toxins for food safety evaluation. The traditional detection method is to separate and prepare the toxin, administer it to mice, and judge it by clinical symptoms, or collect blood, and use expensive Limulus test and high-performance liquid chromatography to detect the toxin. The above two methods are time-consuming, labor-intensive, costly, and the test result...

AI Technical Summary

Problems solved by technology

The above two methods are time-consuming and laborious, the detection cost is high, and the detection results are inaccurate for different types of animals. Therefore, it is urgent to improve the detection method so that it can be applied to animal food safety detection, thereby improving the monitoring and management of food safety.

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