A method for purifying black rabbit coat color based on genotype selection
A purification method and genotype technology, applied in the field of livestock breeding, can solve problems such as incompleteness, time-consuming and labor-intensive, complex genetic background of black rabbits, etc., and achieve the effect of protecting germplasm materials and ensuring uniformity
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Embodiment 1
[0032] A method for purifying black rabbit fur color based on genotype selection, comprising the following steps:
[0033] 1. Extract the genomic DNA of the black meat rabbit, use the first primer set and the second primer set to amplify respectively, and obtain the amplified product;
[0034] The primer design of this example is shown in Table 1.
[0035] Table 1. Sequence map of primer sets
[0036]
[0037] When using the first primer set to amplify, the amplification system is: the total volume is 20 μL, including Tris-HCL3 3.5mM, (NH 4 ) 2 SO 4 8.0mM, MgCl 21.5mM, TWEEN-20 0.05%, dNTPs 0.2mM, forward primer and reverse primer 3.3ng / μl, Taq DNA polymerase 2.0U and template DNA 50ng; the amplification program is: pre-denaturation at 94°C for 2min; Then 30 cycles were performed, each cycle including the following steps in turn: denaturation at 94°C for 45s, annealing at 62°C for 45s, and extension at 72°C for 60s; after completing 30 cycles, extend at 72°C for 8min,...
Embodiment 2
[0046] The difference between embodiment 2 and embodiment 1 is only that the selected breeds of rabbits are different. In this embodiment, black long-haired rabbits are selected as the implementation object, and other operations are the same.
Embodiment 3
[0048] The difference between embodiment 3 and embodiment 1 is only that the selected breeds of rabbits are different. In this embodiment, the black rex rabbit is selected as the implementation object, and other operations are the same.
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