60 results about "Hair Coat Color" patented technology

The invention discloses a method for obtaining sheep with different hair colors on the basis of CRISPR / Cas9 and sgRNA of a targeted ASIP gene. The invention provides sgRNA (ASIP-sgRNA) capable of achieving specific and targeted modification of a sheep ASIP gene, and the sgRNA (ASIP-sgRNA) is RNA as shown from the third nucleotide to the 22<nd> nucleotide of 5' tail end of a sequence 4 of a sequence list or RNA with the nucleotides from the third nucleotide to the 22<nd> nucleotide of the 5' tail end of the sequence 4 of the sequence list. The ASIP-sgRNA particularly can be the RNA as shown in the sequence 4 of the sequence list. The invention further provides a method for obtaining sheep with changed hair colors. The method comprises the following steps that co-transfection of sgRNA capable of achieving specific and targeted modification of the sheep ASIP gene and Cas9mRNA on sheep cells is conducted, and therefore the sheep ASIP gene is deleted, and the sheep with the changed hair colors are obtained. According to the method for obtaining the sheep with different hair colors on the basis of CRISPR / Cas9 and sgRNA of the targeted ASIP gene, a CRISPR / Cas9 genome-editing technology is combined with a microinjection technology, and an effective technological means is provided for artificially changing the hair colors of the sheep.

The invention discloses a fully mixed pellet feed for beef cattle, which is prepared from sugarcane tail tips serving as raw materials. A preparation method comprises the following steps: rapidly drying the sugarcane tail tips until the water content is 20%, and smashing; adding corns, bean pulp, wheat bran, sodium bicarbonate, multivitamins and trace elements; and mixing and pelletizing. The feed is yellow green or light green and has remarkable sugarcane leaf aroma which can attract the beef cattle; strains contained in the feed are helpful to the decomposition of the feed in rumens, so that the nutrients of the feed are absorbed fully. When the beef cattle are bred, if each cow or bull is fed with food accounting for 1%-2% of body weight, the disease resisting rate of the cattle can be enhanced, the cattle are good in hair color and fat, and the weight can be increased obviously.

The invention belongs to the technical field of poultry breeding. A method for breeding a new strain of black-bone chicken with higher production performance comprises the following steps of: step 1, taking parents of foreign broiler chicken as female parents instead of hen CD, and the black-bone chicken with jute feather inland as male parents, and generating a generation F1 through cross breeding, selecting and leaving individuals with jute feather, black bone, and black skin in the generation F1; step 2, crossing and propagating the female individuals and the male individuals obtained in the step 1, and separating out jute feather, recessive white feather, black feather, and reed catkins, and removing recessive white feather, from the colored feather colony through crossing, and purifying and fixing the hair color, black bone, black shin, sexual maturity and other appearance characters according to the family and individual selective method, improving the growing speed, reproductive performance and other economic characters; step 3, through the breeding of four or more generations, successfully breeding the new strain of the black-bone chicken with relatively higher production performance in jute feather, recessive white feather, black feather, and reed catkins. The method improves the early growing speed and egg laying performance of the black-bone chicken in the nation, and obviously reduces the feed / gain ratio.

The invention relates to an experimental animal sign information acquisition and analysis system and a collection and analysis method thereof, which are characterized by comprising a hardware system, a software system and an implementation method. The hardware system comprises a light source, a shading component, a digital camera, a stepper motor for controlling the digital camera to move, a connecting device and a computer. The software system comprises a digital camera moving and shooting control program, a database program, and an image analysis program. The implementation method is that: experimenters operate the system to complete the collection and analysis of data according to instructions. The image analysis program also comprises a tongue image automatic collection and analysis module, a whole hair color collection and analysis module, an ear collection and analysis module, a claw collection and analysis module, and a tail collection and analysis module. The analysis method comprises the following steps of automatic image storage, smart-cut of analysis target images, and automatic extraction and storage of sign data. The system has a scientific design and high degree of automation, is convenient to use, and can be used for a collection and analysis research of various experimental animal sign images, and the obtained experimental data is objective, scientific, and reliable.

The invention relates to food for dogs, and a preparation method thereof; the raw material of the food for dogs is prepared by the following weight proportion: 80 to 100 of beef, 30 to 50 of mutton, 10 to 30 of chicken liver, 10 to 30 of carrot, 2 to 7 of propanediol, 1 to 2 of black kerneled rice, 1 to 2 of red yeast rice, 2 to 8 of soybean flour, 2 to 6 of rice meal and 1 to 4 of corn starch. The preparation method is as follows: fist the carrot is ground into paste; and then the beef, the mutton and the chicken liver are ground into pulp; the corn starch, propanediol, the red yeast rice, the soybean flour, the rice meal and the black kerneled rice are added in, the ground carrot paste is poured in to be evenly stirred, and mixed material is obtained; the mixed material is prepared into blank, and dried for 25h under 75 DEG C. The food for dogs of the invention can meet all nutrition required by the dogs, the palatability of the dogs, so that the hair color of the dogs is brighter. In addition, the adding of black kerneled rice and other crops is good for the supplementation of the trace elements of the dogs and improves the immunity of the dogs.

The invention relates to the treatment field of animal furs and particularly relates to a method of preparing sheepskin clothing face garment leather with a small liquor ratio. The method comprises the following steps: pre-treating soil-species sheepskin in a paddle-tumbler, wherein the pretreatment includes the steps of soaking in water, washing the skin, re-soaking in water, shearing, removing meat and degassing, and a weight ratio of the water to the raw material kin in each pretreatment step is 9-11; then, performing pickling, depickling, re-pickling, tanning, re-tanning, neutralizing and oiling in a rotary drum in sequence, wherein a weight ratio of the water to the raw material skin in each treatment step is 5-7; and performing the processes of drying, moisture regaining, staking, dry cleaning, fine pruning, scalding, dry grinding and clearing to obtain the sheepskin clothing face garment leather. According to the method disclosed by the invention, the soil-species sheepskin is prepared into the sheepskin clothing face garment leather by adopting a small liquid ratio in the paddle-tumbler and the rotary drum, so that the water consumption in the process is reduced, the chemical materials are reduced correspondingly; and the finally prepared sheepskin clothing face garment leather is white in fur color, fine in dermatotome, full, soft and elastic in hand feeling and high in quality.

The invention provides a new-breed large-sized Belgian coat-color rabbit culture method for fur and meat dual use. The culture method comprises the steps that firstly, a male Belgian coat-color rabbitand a female white meat rabbit are subjected to mating to obtain an F1 generation; the male Belgian coat-color rabbit and the female white meat rabbit in the F1 generation are selected for backcrossto obtain an F2 generation; the male Belgian coat-color rabbit and the female white meat rabbit in the F2 generation are selected for backcross again to obtain an F3 generation; the Belgian coat-colorrabbits in the F3 generation are selected for crossing to obtain an F4 generation; the male and female Belgian coat-color rabbits in the F4 generation are selected for gene identification, and male and female homozygotic-type Belgian coat-color rabbits are selected for building a basic group; the basic group is subjected to closed continuous progeny breeding for 3-4 generations, and a new breed of the large-sized Belgian coat-color rabbits with meat and fur dual use, which have identical body shapes and appearances is cultured. By means of the new breed obtained through the culture method, during pure reproduction and hybridization production, it can be guaranteed that the hair colors of the goods-generation rabbits are identical, and the method has high production performance and uniformity and is suitable for a complete set of modernized production of meat rabbits.

The invention discloses pelleted feed for the breeding season of male minks. The pelleted feed comprises components in parts by weight as follows: 40-60 parts of animal nutrients, 10-30 parts of plant nutrients, 5-15 parts of Chinese herbal medicines, 1-5 parts of vitamins, 1-5 parts of microelements, 1-5 parts of zymosan, 1-5 parts of propolis, 2-5 parts of bifidobacterium, 2-5 parts of egg yolk lecithin, 2-5 parts of stachyose, 2-10 parts of methionine-zinc and 2-5 parts of ethoxyquin. A preparation method comprises steps as follows: (1) preparation of raw materials; (2) synthesis of a biological material; (3) conversion into a cooked material; (4) expansion and pelleting; (5) drying; (6) spraying coating; (7) vacuum drying. With the adoption of the feed, the male minks are active during breeding, the breeding rate is high, and the male minks are good in hair color and good in growth condition.

The invention discloses a cultivation method for a long hair rabbit with green, purple and blue hair. A meat rabbit with the green, purple and blue hair and a white Angora rabbit are used as cross parents, and the long hair rabbit with the green, purple and blue hair is cultivated through a cross breeding method, a sib or half-sib mating method, a backcross method and other methods. The cultivation method is short in used time and high in speed. The cultivated long hair rabbit with the green, purple and blue hair is a novel variety with a homozygous genotype including green genes, purple genes, blue genes and Angora hair genes, maintains the body type features of the meat rabbit with the green, purple and blue hair and the hair producing features of the long hair rabbit, has the advantages of being high in utilization rate of feed, high in success rate of cultivation, resistant to coarse feed and strong in disease resistance, and overcomes the defects that a common long hair rabbit is poor in disease resistance and strict in requirement for feeding and management conditions. Due to the fact that the hair color of the cultivated long hair rabbit is natural green, purple and blue, the dyeing process in spinning is eliminated, the cultivation method has the advantages of being energy-saving and environment-friendly, and the cultivated long hair rabbit has a remarkable competitive advantage and market potential in the long hair rabbit breeding industry. Due to the facts that the hair color of the cultivated long hair rabbit is green, purple and blue and the cultivated long hair rabbit has the hair type of the Angora rabbit, the cultivated long hair rabbit also has high viewing value as a pet rabbit.

The invention discloses a breeding method of new Jilin black pigs. The method comprises the following steps: establishing a Min pig base group for propagation, and selecting purified Min pigs meeting requirements; establishing a breeding base group: hybridizing the purified Min pig sows with American Berkshire boars, selecting hybrid pig individuals with all black hair color, establishing an F1 group with clear blood relationship, selecting sows in the F1 group to continue to hybridize with the Berkshire boars to produce an F2 group, and establishing the breeding base group; selecting a breeding core group: selecting 200 sows and 10 boars meeting requirements from the breeding base group to form the breeding core group, and combining genome selection from the breeding core group with a conventional breeding technology to select for multiple times to obtain a black pig core group; and carrying out generation breeding to obtain the black pigs meeting the requirements according to the determination result and the appearance scoring condition. The black pigs have the advantages of being high in litter size, good in meat quality, high in lean meat percentage, fast in growth and development, capable of meeting consumption requirements of China and the like.

The invention relates to a processing technique of animal fur, in particular to a tanning and dyeing technique of mink skin with functions as fur and leather. The technique comprises the following steps of (1) green hide selection and pre-processing, (2) water soaking, freshness recovery, oil removal and grease removal of green hide, (3) nitrate leaching and degreasing, (4) pickling and softening, (5) aluminum tanning, (6) neutralizing, (7) oil tanning, (8) dry cleaning, milling of leather and arranging, (9) chrome tanning, and (10) buffing and dyeing. According to the technique, the design is reasonable, reasonable pre-processing is conducted on the green hide, the quality of the green hide is improved, the subsequent processing technique is simplified, the number of varieties of reagents used for the technique is decreased, the processing cost is low, the production efficiency is high, the quality of finished mink skin is high, the coat color is bright, and the fur is smooth.

The invention provides a culturing method of a new strain of mutton goats with black heads and white bodies. The method is characterized in that a Boer goat is regarded as a male parent, a Macheng black goat is regarded as a female parent, and through a BLUP method, a marker-assisted selection (MAS) technology and combination of a conventional culturing method, by means of crossing fixup and embryo transplantation and propagation, the new strain of mutton goats with a high reproductive rate, a good meat performance and high temperature and humidity resistance are selected finally. According to the culturing method, the Macheng black goat is regarded as the female parent, the Boer goat is regarded as the male parent, and through the BLUP method, a multiple genes pyramiding technology and the combination of the conventional culturing method, the new strain of mutton goats with hair colors, high reproductive rate, meat performance and high temperature and humidity resistance is cultured; the characteristic of the black heads and white bodies is stable, the growth and meat performances of the new strain of the mutton goats are much higher than those of the Macheng black goat, the growth performance, the crude feed resistance, the disease resistance and the stress tolerance are higher than those of the Boer goat, and the production performance level of modern mutton goat breeds is achieved.

The invention provides a purification method for domestic rabbits with a Belgian hair color based on genotype selection. The purification method is applicable to purification of various domestic rabbits with the Belgian hair color, such as meat rabbits, rex rabbits, long hair rabbits and ornamental rabbits. The method provided by the invention comprises the following steps: firstly, selecting thedomestic rabbits with a hair color phenotype which is the Belgian hair color; then detecting an ASIP (Agouti Signaling Protein) gene, an MC1R (Melanocortin 1-Receptor) gene and a TYR (Tyrosinase) geneat the same time by utilizing a first primer group, a second primer group and a third primer group; screening the domestic rabbits with genotypes of AA (corresponding to the ASIP gene), EE (corresponding to the MC1R gene) and CC (corresponding to the TYR gene). The domestic rabbits with the genotype of AAEECC, which are obtained by the purification method, are Belgian hair color homozygotes and recessive white genes are eliminated. According to a domestic rabbit specialized line with the Belgian hair color, which is cultivated by the purification method, germplasm materials of breeding enterprises can be protected very well on the basis of ensuring that the hair colors of commercial rabbits are consistent.

The invention provides a molecular marker related to white hair phenotypes of buffaloes and an application. The molecular marker is developed based on buffalo whole genome association analysis, and comprises 2 SNP molecular markers, namely T / C mutation of the 101st base of the sequence shown in SEQ ID NO.1 and G / A mutation of the 101st base of the sequence shown in SEQ ID NO.2. The molecular marker provided by the invention can be used for genetically identifying white hair phenotypes and tile grey hair phenotypes of buffaloes, can be used for auxiliary breeding of white hair buffaloes havingbuffalo or buffaloes having tile grey hair, and has a good application prospect.

The invention relates to a pet feed which is prepared from maltodextrin, starch, amino acids essential to pets, vitamins, mineral substances, plant roots and stems, gourmet powder, table salt, fatty acid, soybean flour, algae powder, lysine, sodium ascorbate, bone meal, carrot powder and fruit of Chinese wolfberry by uniformly mixing, grinding, preprocessing and mixing. Shelf life of the pet feed is longer than two years. The pet feed is applicable to domesticated pet dogs and cats, also can be used for professional pet farms, and is functional nutrition powder capable of boosting immunity, relieving separation anxiety, reducing depression and relieving emotion of pets; the pet feed disclosed by the invention, as the best natural nutritional supplement, is high in nutrition value and free of food additives; fed with the pet feed, the pet is bright in hair color, good in spirit and active and both immunity and disease resistance are greatly improved.

The invention relates to the technical field of pet food, and particularly relates to puffed pet food and a preparation method thereof. The puffed pet food is prepared from the following components inparts by weight: 200 parts of corn flour, 300 parts of rice flour, 180 parts of chicken meal, 100 parts of beef and bone meal, 100 parts of chicken oil, 20 parts of enzymolysis fresh meat, 50 parts of meat pulp, 20 parts of hydrolyzed chicken liver powder, 13 parts of beef tallow, 2 parts of probiotics, 5 parts of lecithin and 10 parts of vitamins and trace elements. The preparation method of thepuffed pet food comprises the following steps of: mixing, crushing, secondary mixing, tertiary mixing, puffing, drying, spraying oil, cooling, spraying powder and packaging. The puffed pet food has the beneficial effects that the food intake of a pet is increased; the fragrance of various meat is retained, and the digestion and absorption of various meat are improved; the hair color of the pet isimproved; the palatability of the pet is improved; the intestinal flora of the pet is improved, and the reproduction of intestinal probiotics is promoted; and the digestion and absorption of nutrientsubstances are promoted.

The invention provides a breeding method of pure-black-hair-color pig breeds. The method is mainly characterized in that the specific loci (the 708bp locus, 730bp locus and 788bp locus of the MC1R*31gene) of male parent and / or female parent pig breeds are detected, and offspring pig hair color can be predicted accurately by judging genotype to obtain the pure-black-hair-color offspring.

The invention discloses a method for identifying a golden silk yak variety by using an MC1R gene mononucleotide genetic marker. According to the invention, two methods of whole-genome association analysis and whole-genome selective clearance signal calculation are utilized to analyze the whole-genome SNP of the golden-hair Yuyak, and finally a specific mutation which enables MC1R gene codons to terminate in advance on the 20# chromosome of the Yuyak is identified, so that a basis is provided for variety identification of Yuyak. Meanwhile, according to the SNP site where the mutation is located, molecular marker-assisted selective breeding can be carried out on the golden hair color character of the Yuyak on the DNA level, so that a golden yak population with excellent genetic resources is rapidly established.

The invention discloses a breeding pig offspring hair color prediction method based on a haplotype MC1R*31 gene. The breeding pig offspring hair color prediction method comprises the following steps of detecting at least one of a 661bp site, a 721bp site and a 781bp site of the haplotype MC1R*31 gene of a male parent and / or female parent breeding pig, and accurately predicting offspring hair color of a specialized line and a new variety (commercial line) based on a plurality of sites of the MC1R*31 gene. thereby ensuring the black hair color of the bred offspring breeding pigs. Compared with a traditional breeding technology, the method has the advantages that the breeding period can be rapidly shortened, the breeding cost of the breeding pigs can be reduced, and the consistency and economic value of the hair colors of the offspring breeding pigs or commercial pigs can be improved.

The invention relates to the technical field of pet food, in particular to a natural dog food composition capable of protecting skin and brightening hair and a preparation method of the natural dog food composition. The invention discloses a natural dog food composition for protecting skin and brightening hair; the composition at least comprises the following components by weight parts: 5-20 partsof flaxseeds, 5-15 parts of sunflower seeds, 3-7 parts of castor seeds, 3-10 parts of rapeseeds, 8-15 parts of methionine, 5-15 parts of cystine, 1-5 parts of beewax, 0.1-2 parts of tea leaves, 1-5 parts of Chinese wolfberry, 5-15 parts of jujubes, 1-5 parts of laver, 2-8 parts of undaria pinnatifida, 1-5 parts of spinach, 10-20 parts of buckwheat and 15-30 parts of corn. The dog food prepared bythe invention can protect hair follicles and hair of dogs from being damaged, enable the hair of dogs to be smooth and gentle, prevent bifurcation, reduce hair loss, promote fur regeneration, enablethe hair to grow vigorously, brighten the hair color, improve the hair quality, enable the hair to have elasticity, prevent pilling of the hair, reduce the incidence of skin diseases and improve skinallergy and pruritus.

The present invention discloses a feed additive for improving hair quality and hair color of cats and a preparation method thereof. The feed additive consists of the following raw materials: fructus alpiniae oxyphyllae, metaplexis japonica, radix polygonati officinalis, dandelions, saussurea involucrata flowers, sedum aizoon, siberian nitraria fruits, white perilla stems, ashycoloured ironweed herb, lucid ganoderma, melodinus suaveolens fruits and rhizoma curcumae; and preferably, further comprises the following raw materials: ovateleaf holly bark, cassia seeds, ustilago nuda, Chinese flowering quince fruits and stems or leaves of fortune euonymus. The beneficial effects are as follows: the feed additive can comprehensively improve the health of cats' skin-hair, protect hair follicle and hair from damages, enable hair to be bright and smooth, reduce hair loss, promote regeneration of skin-hair, enhance immunity of the cats, and enable the cats' skin-hair to be smooth and bright, bright in color, and smooth and flexible, and can increase hair quality and brighten hair color. The raw materials are all natural products, so that the feed additive has advantages of being high in security, free of drug residues, toxic or side effects, etc.

The invention provides a rapid and accurate coat color trait fixing method and a rapid breeding method for a new black pig variety. The rapid and accurate coat color trait fixing method comprises thefollowing steps: firstly, observing and counting male parent, female parent, F1 generation and F2 generation coat color phenotypes and proportions of the bred black pig variety to determine that coatcolor inheritance of the variety belongs to single-gene control; secondly, screening genes for controlling the coat color, and preliminarily determining a major gene (the major gene of black coat is an MC1R gene); then performing extraction, amplification and sequencing to obtain the genotype of the major gene (the F2 generation genotype black homozygote is E<D1>E<D1>, the heterozygote is E<D1>E and the black and white spotted pig is EE), and meanwhile, determining that the hair color of the cultivated variety is dominant heredity under single-gene control; and finally, breeding blackpigs with black homozygote (E<D1>E<D1>) genotypes reserved in the F2 generation so as to fix the coat color in the F3 generation. The rapid and accurate coat color trait fixing method and the rapid breeding method overcome the defects of long consumed time and low purity of conventional phenotypic breeding, and have the advantages of fast breeding process, high breeding accuracy and the like.

The invention discloses small interfering ribonucleic acid (siRNA) capable of interfering alpaca melanin cell CDK5 gene expression. The siRNA has positive-sense strand and antisense strand sequences as shown in Seq ID No. 1 and Seq ID No. 2; a pENTR / U6 short hairpin RNA (shRNA) expression plasmid capable of interfering the alpaca melanin cell CDK5 gene expression is also established. The biological effect of the pENTR / U6 (shRNA) expression plasmid shows a regulating function for alpaca melanin cell lines TYR and MC1R in a melanin cell line as well as an influence on phenotypic change of hair color of a mouse.

The invention discloses a breeding method of Kelepigs. The breeding method of the Kelepigs comprises the following steps of (1) establishing basic groups, specifically, introducing the Kelepigs with hair color characteristics of black, brown and six white from a Kelepig distribution area to form corresponding zero-generation basic groups; (2) carrying out closed breeding, specifically, carrying out line group closing on the zero-generation basic groups with the corresponding hair color characteristics; (3) carrying out open type breeding, specifically, introducing Kelepig external blood to participate in breeding during the open type breeding; and (4) carrying out the closed breeding again, specifically, carrying out line group closing again on the population with corresponding hair color characteristics in the fifth generation, only allowing boars and sows in the groups to mate and breed, and not allowing to introduce a Kele pig blood source to the outside. In the steps, individuals containing Halnn and HalNn genotypes and carrying RN genes are removed by adopting a PCR-RFLP technology at the same time, and group subculture breeding, stage selection and homogeneous matching are used for cross-crossing fixation until black Kelepigs, the brown Kelepigs and the six-white Kelepigs which are stable in heredity are bred.

The invention belongs to the technical field of goat breeding, and discloses an artificial breeding method for goats, which comprises the following steps: selecting breeding ewes and breeding rams; according to the pedigree information, the body shapes, appearances and production characteristics of the rams and the quality characteristics of the offspring sheep, the first rams in the field are selected for training before artificial semen collection; the estrus cycle of the breeding ewes is controlled and adjusted through hormones; semen collection is conducted on the breeding rams; and delivering the collected quantitative high-quality seminal fluid into the cervix of the ewe with the oestrus. Reserve ewes and nonpregnant ewes which are sexually mature, good in health condition, free of mastitis, strong in physique and all black in hair color are selected as breeding ewes. According to the artificial breeding method for goats, the utilization rate of high-quality rams can be increased, the conception rate is increased, and diseases are reduced; the feeding cost is reduced and the benefit is improved.

The invention provides sgRNA for specific recognition of a pig KIT gene, a coding DNA thereof and a kit, and application thereof, belonging to the technical field of gene engineering. In the sgRNA, anucleotide sequence responsible for identifying a target fragment region is a sequence as shown in SEQ ID NO.1, a sequence as shown in SEQ ID NO.2 or a sequence as shown in SEQ ID NO.3; and the sgRNAenables a CRISPR / Cas9 gene editing system to delete redundant mutation copies of the KIT gene on a pig genome, so the KIT gene can normally express KIT protein with biological activity, and the hair color of a pig is changed.

The invention discloses a method for identifying a spotted tumor cattle variety by using a KIT gene copy number variation genetic marker. The variety identification of spotted tumor cattle is realized according to a CNV section which contains about 490Kb of a KIT gene and is subjected to repeated mutation on a cattle chromosome 6 found by carrying out whole genome CNV analysis on spotted hair color character tumor cattle and other pure color hair color character cattle varieties. According to the CNV segment, molecular marker-assisted selection can be carried out on tumor cattle hair color characters on the DNA level, so that a population with excellent genetic resources is rapidly established.

The invention discloses a pig-breeding method through lighting. In a general pig-breeding process, besides normal raising, pigs are periodically irradiated with lamp light in the pig-breeding process,the color temperature of the lamp light is 2120K, the main wavelength is 594.3nm, the peak wavelength is 618.8nm, the color purity is 56.1%, and the color ratio lies in that R is 34.2%, G is 64.4% and B is 1.4%, and the illumination intensity of the lamp light is 40Lux-50Lux. Periodical irradiation is performed on the pigs in the breeding process, feeds are well absorbed by the pigs, and the haircolor of the pigs is good. The period that piggies become full-grown and ready for slaughter is short, the period that the piggies become full-grown and ready for slaughter can be advanced by at least 10 days or above, and besides, in the entire breeding process, only irradiation is adopted, so that the pig-breeding method is healthy, and the mortality rate of the pigs can be greatly reduced.

The invention relates to a laying hen feed and a preparation method thereof. The laying hen feed disclosed by the invention is prepared by using methionine, lysine, multiple vitamins, feed sodium chloride, sodium bicarbonate, monocalcium-dicalcium phosphate, vitamin E, an antioxidant, compound trace elements, phytase, bacillus subtilis, rice husks, choline chloride, calcium formate, calcium lactate and calcium butyrate as raw materials through adopting a multistage mixing method. According to the feed, a proportion and cost of a premix are reduced, and nutritional requirements of laying hens in each of breeding stages can be met, i.e., in the early period of egg laying, common nutritional requirements of egg laying and growth are met, in the middle period of egg laying, the laying hens arewell-proportioned in physique and peak holding time is long, and in the later period of egg laying, the laying hens are good in hair color and good in physique and eggshells are good; health care nutritional raw materials are added, and when nutrients are provided, a health stage of the laying hens in the breeding process is effectively improved, so that the laying hens keep a long peak period, and are good in hair color and strong in physique in the later period of breeding; and the eggshells are high in quality, ruddy and glossy.

The invention provides a selecting method for domestic rabbits with a agouti / Belgian hair color based on homozygous genotype selection. The purification method is applicable to purification of various domestic rabbits with the agouti / Belgian hair color. The method includes the following steps: firstly, selecting the domestic rabbits with a hair color phenotype which is the agouti / Belgian hair color; then detecting an ASIP gene, a MC1R gene and a TYR gene at the same time by utilizing a first primer set, a second primer set and a third primer set; screening the domestic rabbits with genotypes of AA (corresponding to the ASIP gene), EE (corresponding to the MC1R gene) and CC (corresponding to the TYR gene). The domestic rabbits with the genotype of AAEECC, which are obtained by the screening method of the invention, are agouti / Belgian hair color homozygotes and the recessive white gene is eliminated.