Method for identifying golden silk yak variety by using MC1R gene mononucleotide genetic marker

Abstract

The invention discloses a method for identifying a golden silk yak variety by using an MC1R gene mononucleotide genetic marker. According to the invention, two methods of whole-genome association analysis and whole-genome selective clearance signal calculation are utilized to analyze the whole-genome SNP of the golden-hair Yuyak, and finally a specific mutation which enables MC1R gene codons to terminate in advance on the 20# chromosome of the Yuyak is identified, so that a basis is provided for variety identification of Yuyak. Meanwhile, according to the SNP site where the mutation is located, molecular marker-assisted selective breeding can be carried out on the golden hair color character of the Yuyak on the DNA level, so that a golden yak population with excellent genetic resources is rapidly established.

Description

technical field [0001] The invention relates to molecular marker-assisted identification in the introduction and breeding of yaks (Bos grunniens), in particular to the detection of SNP markers closely related to the golden coat color traits of yaks at the DNA level, and the use of MC1R gene single nucleotide genetic markers (SNP markers) Identification of Yushu Golden Yak. Background technique [0002] Single nucleotide polymorphism (SNP, single nucleotide polymorphism) is a phenomenon in which a single nucleotide differs in the allelic sequence of the genomic DNA of different individuals of the same species. The single-stranded DNA of the same length will have different conformations due to the difference in sequence or single base difference. SNP is the most widespread type of variation in the genome of organisms, which is caused by the insertion, deletion, conversion and transversion of a single nucleotide. [0003] The existence of certain SNPs leads to the retention o...

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Problems solved by technology

[0005] At present, there is no research on the genes related to the golden coat color of the golden silk wild yak, especially the main gene of the coat color of the Yushu golden yak has not yet been identified. Yushu golden silk wild yak) are often chimerized with each other, and the corresponding traits are gradually obvious when the proportion of golden coat color increases, which makes it more difficult to screen the genetic loci that determine the traits of golden coat color, and is easily affected by the results of other coat color genes. interference

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