Rapid and accurate coat color trait fixing method for breeding new black pig variety and application

Abstract

The invention provides a rapid and accurate coat color trait fixing method and a rapid breeding method for a new black pig variety. The rapid and accurate coat color trait fixing method comprises thefollowing steps: firstly, observing and counting male parent, female parent, F1 generation and F2 generation coat color phenotypes and proportions of the bred black pig variety to determine that coatcolor inheritance of the variety belongs to single-gene control; secondly, screening genes for controlling the coat color, and preliminarily determining a major gene (the major gene of black coat is an MC1R gene); then performing extraction, amplification and sequencing to obtain the genotype of the major gene (the F2 generation genotype black homozygote is E<D1>E<D1>, the heterozygote is E<D1>E and the black and white spotted pig is EE), and meanwhile, determining that the hair color of the cultivated variety is dominant heredity under single-gene control; and finally, breeding blackpigs with black homozygote (E<D1>E<D1>) genotypes reserved in the F2 generation so as to fix the coat color in the F3 generation. The rapid and accurate coat color trait fixing method and the rapid breeding method overcome the defects of long consumed time and low purity of conventional phenotypic breeding, and have the advantages of fast breeding process, high breeding accuracy and the like.

Description

technical field [0001] The invention relates to the technical fields of animal quantitative genetics and molecular biology, in particular to a method for quickly and precisely fixing the coat color traits of a new breed of black pig and its application. Background technique [0002] The breeding of new breeds of pigs today mainly utilizes hybrid vigor to integrate the excellent characteristics of various breeds of pigs to achieve balanced selection of multiple traits. The breeding of new breeds of pigs in my country retains the advantages of strong maternal sex, obvious estrus, high fecundity, good meat quality, strong adaptability and stress resistance of local breeds of pigs in my country, and improves their slow weight gain, poor body structure, low slaughter rate, and lean meat rate. Low disadvantages, combined the advantages of different varieties, improved growth and development, carcass and pork quality. In the cultivation of new varieties, the cultivated varieties re...

AI Technical Summary

Problems solved by technology

[0007] In view of this, the object of the present invention is to propose a method for fixing the hair color of a new breed of black pig and a rapid breeding method, so as to solve the problems of long timeliness and low purity in the existing hair color fixing and breeding.

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