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Purification method for domestic rabbits with Belgian hair color based on genotype selection

A purification method and genotype technology, applied in the field of livestock breeding, can solve problems such as insufficient thoroughness, time-consuming and laborious, complex genetic background, etc., and achieve the effects of ensuring product consistency, protecting germplasm materials, and ensuring uniformity.

Active Publication Date: 2019-04-26
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rabbit coat color is controlled by multiple genes, and these genes show complex dominant-recessive and epistatic interactions. In production practice, the genetic background of Belgian coat-color rabbits is relatively complicated, and there are few homozygous populations of coat color. Using conventional breeding methods Purification of hair color is not only time-consuming and laborious, but also often incomplete

Method used

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  • Purification method for domestic rabbits with Belgian hair color based on genotype selection
  • Purification method for domestic rabbits with Belgian hair color based on genotype selection
  • Purification method for domestic rabbits with Belgian hair color based on genotype selection

Examples

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Embodiment 1

[0037] A method for purifying Belgian fur-colored rabbits based on molecular-assisted selection, comprising the following steps:

[0038] 1. Extract the genomic DNA of the Belgian colored meat rabbit, amplify using the first primer set, the second primer set and the third primer set respectively to obtain the amplified product;

[0039] The primer design of this example is as shown in table 1;

[0040] Table 1. Sequence map of primer sets

[0041]

[0042] When using the first primer set to amplify, the amplification system is: the total volume is 20 μL, including Tris-HCL3.5mM, MgCl 2 25mM, dNTPs 0.2mM, forward primer and reverse primer 5ng / μl, Taq DNA polymerase 2.5U and template DNA 50ng; the amplification program is: pre-denaturation at 94°C for 5min; then 30 cycles, in which each Each cycle includes the following steps in turn: denaturation at 94°C for 45 s, annealing at 52°C for 45 s, and extension at 72°C for 30 s; after completing 30 cycles, extend at 72°C for 5 ...

Embodiment 2

[0053] The difference between embodiment 2 and embodiment 1 is only that the selected breeds of rabbits are different. In this embodiment, Belgian long-haired rabbits are selected as the implementation object, and other operations are the same.

Embodiment 3

[0055] The difference between embodiment 3 and embodiment 1 is only that the selected breeds of rabbits are different. In this embodiment, Belgian-colored rex rabbits are selected as the implementation object, and other operations are the same.

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Abstract

The invention provides a purification method for domestic rabbits with a Belgian hair color based on genotype selection. The purification method is applicable to purification of various domestic rabbits with the Belgian hair color, such as meat rabbits, rex rabbits, long hair rabbits and ornamental rabbits. The method provided by the invention comprises the following steps: firstly, selecting thedomestic rabbits with a hair color phenotype which is the Belgian hair color; then detecting an ASIP (Agouti Signaling Protein) gene, an MC1R (Melanocortin 1-Receptor) gene and a TYR (Tyrosinase) geneat the same time by utilizing a first primer group, a second primer group and a third primer group; screening the domestic rabbits with genotypes of AA (corresponding to the ASIP gene), EE (corresponding to the MC1R gene) and CC (corresponding to the TYR gene). The domestic rabbits with the genotype of AAEECC, which are obtained by the purification method, are Belgian hair color homozygotes and recessive white genes are eliminated. According to a domestic rabbit specialized line with the Belgian hair color, which is cultivated by the purification method, germplasm materials of breeding enterprises can be protected very well on the basis of ensuring that the hair colors of commercial rabbits are consistent.

Description

technical field [0001] The invention belongs to the technical field of livestock breeding, in particular to a method for purifying Belgian fur-colored rabbits based on genotype selection. Background technique [0002] The fur color of Belgian rabbit belongs to the wild type. The coat is dark red with yellowish brown or reddish brown. The ends of the whole hair are deep and the middle is lighter. The texture is tough and clings to the body surface. The hair fibers on the head, neck, back, body side and outside of the limbs are similar in color to the hare hair fibers. The base of the hair fibers is light gray, the middle part is lighter, the hair tips are darker, and mixed with black gun hair. The hair color on the lower jaw, under the neck, the inside of the limbs, the abdomen, and the ventral surface of the tail is lighter. The skin is dark gray except for the abdomen, inner sides of the limbs and under the jaw, and the other parts are dark gray, and the coat color is brig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156A01K67/02
Inventor 樊新忠乔西波惠春晖王波杨爱国胡家卿李明勇
Owner SHANDONG AGRICULTURAL UNIVERSITY
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