Molecular marker related to white hair phenotypes of buffaloes and application

A molecular marker, buffalo technology, used in animal breeding, genetics and molecular biology to achieve the effect of accurate methods

Active Publication Date: 2021-01-12
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the genetic mechanism of buffalo white coat color and th

Method used

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  • Molecular marker related to white hair phenotypes of buffaloes and application
  • Molecular marker related to white hair phenotypes of buffaloes and application
  • Molecular marker related to white hair phenotypes of buffaloes and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Obtaining Molecular Markers Related to Buffalo White Coat Color Phenotype

[0031] 1. Sample collection

[0032] In the buffalo producing area in southern China, 63 swamp-type buffaloes were randomly collected, including 41 tile-gray buffaloes and 22 white buffaloes. The sample is the ear epithelial tissue, which is placed in a sample tube with 75% alcohol and stored at -80°C.

[0033] 2. Buffalo Genomic DNA Extraction

[0034] (1) Solution preparation: tissue lysate (1L): 1.2114g solid Tris-Base (121.1), 37.22g solid disodium ethylenediaminetetraacetic acid (EDTA, 372.24), 5.0g solid sodium dodecyl sulfate (SDS ) was dissolved in 800mL of double distilled water, after complete dissolution, additional double distilled water was added to make the volume to 1L, and the pH was adjusted to 8.0.

[0035] Proteinase K solution (20mg / mL): 100mg protein K yellow crystals, dissolved in 5mL deionized water, aliquoted and stored at -20°C.

[0036] (2) Take 200 mg of ...

Embodiment 2

[0062] Example 2 Verification of SNP marker g.9953331

[0063] The g.9953331 site genotyping results are shown in Table 1.

[0064] Table 1 SNP site g.19953331 genotype frequency distribution

[0065]

[0066] *Note: C-mutant, T-normal.

[0067] According to the statistical results of the data of the present invention, when the g.19953331 marker genotype is CC, there is 100% confidence that the individual can be inferred to be white coat color, and when the g.19953331 marker genotype is CT, there are 95.2% (20 / 21×100% =95.2%), it can be inferred that the individual has a white coat color, and when the g.19953331 marker genotype is TT, it can be inferred that the individual is a tile-gray coat color with 100% confidence.

Embodiment 3

[0068] Embodiment 3 Verification of SNP marker g.19970628

[0069] The g.19970628 site genotyping results are shown in Table 2.

[0070] Table 2 SNP site g.19970628 genotype frequency distribution

[0071]

[0072] *Note: A-mutant, G-normal.

[0073] According to the statistical results of the data of the present invention, when g.19970628 marker genotype is AA, there is 100% certainty that the individual can be inferred to be white coat color; when g.19970628 marker genotype is AG, there are 95.2% (20 / 21×100% =95.2%), it can be inferred that the individual has a white coat color, and when the g.19970628 marker genotype is GG, it can be inferred that the individual is a tile-gray coat color with 100% confidence.

[0074] Therefore, the two marker genotypes can be detected in the Chinese buffalo population, and the retention of white homozygous buffalo individuals can rapidly increase the proportion of homozygous white coat color genes.

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Abstract

The invention provides a molecular marker related to white hair phenotypes of buffaloes and an application. The molecular marker is developed based on buffalo whole genome association analysis, and comprises 2 SNP molecular markers, namely T/C mutation of the 101st base of the sequence shown in SEQ ID NO.1 and G/A mutation of the 101st base of the sequence shown in SEQ ID NO.2. The molecular marker provided by the invention can be used for genetically identifying white hair phenotypes and tile grey hair phenotypes of buffaloes, can be used for auxiliary breeding of white hair buffaloes havingbuffalo or buffaloes having tile grey hair, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical fields of animal breeding, genetics and molecular biology, and in particular relates to molecular markers and applications related to buffalo white coat color phenotype. Background technique [0002] Domestic buffaloes are divided into two main types—river type and swamp type. In my country, swamp type buffalo is the main type. Swamp buffaloes have two common coat colors—tile gray and white. The body of tile gray buffalo is tile gray, the lower parts of the limbs are lighter in color, grayish white, and there are light gray or white stripes on the lower neck and chest; the number of white buffaloes is small, The whole body is white with pink skin and black eyes. So far, the genetic mechanism of buffalo white coat color and the method of molecular marker-assisted selection of white coat color have not been reported at home and abroad. [0003] The study on the gene mapping and mechanism of buffalo white coat colo...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张毅梁栋司敬方韩建林龚俞
Owner CHINA AGRI UNIV
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