Specific primer and liquid phase chip for detecting TYR gene mutation

Abstract

The invention discloses a liquid phase chip and a specific primer for detecting TYR gene mutation. The liquid phase chip mainly comprises: ASPE primers, microspheres coated by different anti-tag sequences, and an amplification primer, wherein each ASPE primer is composed of a tag sequence at a 5' end and specific primer sequences at a 3' end and aiming at target gene mutation sites, and the specific primer sequences are as follows: SEQ ID NO.9 and SEQ ID NO.10 aiming at a G6895A site, SEQ ID NO.11 and SEQ ID NO.12 aiming at an C1205T site, SEQ ID NO.13 and SEQ ID NO.14 aiming at a C575A site, and / or SEQ ID NO.15 and SEQ ID NO.16 aiming at an A99214C site. The coincidence rate of the detection result of the detection liquid phase chip disclosed by the invention with that of a sequencing method reaches as high as 100%, and parallel detection of a wild type and a mutant of a plurality of mutation sites is achieved.

Description

technical field

[0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, in particular to a TYR gene mutation detection specific primer and a liquid phase chip. Background technique

[0002] Tyrosinase (TYR) is located on the long arm of chromosome 11 11q14.3, specifically between 88911039 and 89028926 base pairs on chromosome 11. Tyrosinase (TYR) is a 75kD copper-containing enzyme derived from embryonic neural crest cells and is a key enzyme in melanin metabolism and catecholamines. Studies have found that more than 100 mutations of the TYR gene can be found in patients with oculocutaneous albinism type I. These mutations disrupt the normal production of melanin, thereby reducing the coloration of hair, skin and eyes. In addition, tyrosinase may be an important antigen of vitiligo autoimmunity. It has recently been found that some patients with vitiligo have tyrosinase antibodies in their serum, which are closely related to th...

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