Black rabbit hair color purification method based on genotype selection

A purification method and genotype technology, applied in the field of livestock breeding, can solve the problems of insufficient thoroughness, time-consuming and labor-intensive, few populations with homozygous coat color, etc., and achieve the effect of protecting germplasm materials and ensuring uniformity

Active Publication Date: 2019-05-14
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

The coat color of the germplasm material rabbit is controlled by multiple genes, and these genes show complex dominant-recessive and epistatic interactions. In production practice, the genetic background of black rabbits is relatively complex, and there are few homozygous coat color populations. Using conventional breeding The method of hair color purification is not only time-consuming and laborious, but also often not thorough enough

Method used

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  • Black rabbit hair color purification method based on genotype selection
  • Black rabbit hair color purification method based on genotype selection
  • Black rabbit hair color purification method based on genotype selection

Examples

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Embodiment 1

[0032] A method for purifying black rabbit fur color based on genotype selection, comprising the following steps:

[0033] 1. Extract the genomic DNA of the black meat rabbit, use the first primer set and the second primer set to amplify respectively, and obtain the amplified product;

[0034] The primer design of this example is shown in Table 1.

[0035] Table 1. Sequence map of primer sets

[0036]

[0037] When using the first primer set to amplify, the amplification system is: the total volume is 20 μL, including Tris-HCL3 3.5mM, (NH 4 ) 2 SO 4 8.0mM, MgCl 21.5mM, TWEEN-20 0.05%, dNTPs 0.2mM, forward primer and reverse primer 3.3ng / μl, Taq DNA polymerase 2.0U and template DNA 50ng; amplification program: pre-denaturation at 94°C for 2min ; Then perform 30 cycles, each of which includes the following steps in turn: denaturation at 94°C for 45s, annealing at 62°C for 45s, and extension at 72°C for 60s; after completing 30 cycles, extend at 72°C for 8min , that is,...

Embodiment 2

[0046] The difference between embodiment 2 and embodiment 1 is only that the selected breeds of rabbits are different. In this embodiment, black long-haired rabbits are selected as the implementation object, and other operations are the same.

Embodiment 3

[0048] The difference between embodiment 3 and embodiment 1 is only that the selected breeds of rabbits are different. In this embodiment, the black rex rabbit is selected as the implementation object, and other operations are the same.

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PUM

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Abstract

The invention provides a black rabbit hair color purification method based on genotype selection. The purification method is suitable for purifying the hair color of various black rabbits such as meatrabbits, rex rabbits, long-hair rabbits, ornamental rabbits and experimental rabbits. The method includes the steps that rabbits with a black hair color phenotype are picked out; then, a first primergroup and a second primer group are used for detecting an MC1R gene and a TYR gene simultaneously, and black rabbits with the genotype being EDED (corresponding to the MC1R gene) and CC (corresponding to the TYR gene) are screened out. The obtained black rabbits are homozygous, recessive white genes are removed while EDEd heterozygotes are removed, and the double insurance of obtaining homozygousdominant black genes and removing recessive white genes is realized in breeding rabbit hair color selection. It can be guranateed that offspring are all black rabbits after the black rabbits obtainedthrough the method are mated with rabbits with other hair colors. The black rabbit specialized line cultured by the purification method can well protect the germplasm material of a breeding enterprise on the basis of ensuring the uniform hair color of commercial rabbits.

Description

technical field [0001] The invention belongs to the technical field of livestock breeding, and in particular relates to a method for purifying the fur color of black rabbits based on genotype selection. Background technique [0002] Black rabbit refers to the rabbit whose coat color and eyeball color are black. Among all kinds of rabbits such as meat rabbits, rex rabbits, long-haired rabbits, and ornamental rabbits, black is one of the most common rabbit coat colors. Black not only serves as an easily identifiable breed characteristic of rabbits, but also has specific economic value, social needs and market preferences. The coat color of the germplasm material rabbit is controlled by multiple genes, and these genes show complex dominant-recessive and epistatic interactions. In production practice, the genetic background of black rabbits is relatively complex, and there are few homozygous coat color populations. Using conventional breeding The method of hair color purificati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 樊新忠乔西波惠春晖王波杨爱国胡家卿李明勇
Owner SHANDONG AGRICULTURAL UNIVERSITY
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