38 results about "Paramisgurnus dabryanus" patented technology

An artificial propagation method for paramisgurnus dabryanus first-filial generation relates to the technical field of aquiculture and comprises the following steps: parent cultivation, pond management, artificial propagation, egg laying and hatching and in-time fishing. The artificial propagation method has the benefits that the production resource is fully utilized, special equipment is not required, raising pollution is avoided, the raising technology is standard and normalized, feeding is scientific and normalized, the technology is novel, the aquiculture quality is improved to a great extent, and accordingly, the profit is increased.

The invention discloses a preservation solution, an activation solution and a hatching method for Paramisgurnus dabryanus sperms, belonging to the technical field of aquaculture. The sperm preservation solution comprises the following components: 0.5% to 1.0% of trisodium citrate, 1.0% to 3.0% of glucose, 0.1% to 0.3% of sodium bicarbonate, 0.02% to 0.04% of potassium chloride and 0.01% to 0.02% of VC; the activation solution comprises the following components: 1 to 2% of glucose and 0.35% of sodium chloride; and the hatching method comprises the following steps: activating sperms preserved with the sperm preservation solution by using the activation solution, allowing the sperms to combine with eggs, placing fertilized eggs on a mesh, and carrying out hatching in static water. Compared with the prior art, the invention has the following advantages: through innovation of the activation manner of the Paramisgurnus dabryanus sperms in a specific sperm preservation solution, the maximum vitality of the sperms can be guaranteed; the fertilization rate and the hatching rate of fish eggs are improved; and the fry breeding production is guided.

The invention discloses a method for producing allopolyploid misgurnus anguillicaudatus through inhibiting fertilized egg polar body release. According to the method, diploid misgurnus anguillicaudatus, tetraploid misgurnus anguillicaudatus and paramisgurnus dabryanus are adopted as propagation parents and are used for hybrid fertilized eggs, such that the hybrid fertilized eggs are obtained; on the 5th minute after the hybrid fertilized eggs are obtained, the hybrid fertilized eggs are soaked in ice water with a temperature of 2-3 DEG C for cold shock treatment with a duration of 30-35min; and the eggs are hatched and cultured under room temperature, such that misgurnus anguillicaudatus allopolyploids are obtained. With the method, allotriploid misgurnus anguillicaudatus, allotetraploid misgurnus anguillicaudatus and allopentaploid misgurnus anguillicaudatus can be obtained. With the method, the induced misgurnus anguillicaudatus allopolyploid variety number is high, and the amount is high. The induction success rate is high, and the offspring growth is fast. With the method, the weight of 12-month-age misgurnus anguillicaudatus is increased by an average of -19.99% to 142.34% than that of a normal selfing control group. The weight gain effect is significant. The method has high popularization benefit.

The invention discloses a grading cultivation method of paramisgurnus dabryanus fries. The method includes carrying out primary cultivation on yolk fries of the paramisgurnus dabryanus to a total length of 1-1.5cm under a density of 50000 tails/cubic meter; then carrying out secondary cultivation to a total length of 2-2.5cm under a density of 10,000-20,000 tails/cubic meter;and finally, carryingout tertiary cultivation to a total length of 4-5cm under a density of 400-500 tails/cubic meter. According to the method, before the yolk fries of the paramisgurnus dabryanus grows to 2-2.5cm, the yolk fries of the paramisgurnus dabryanus is cultivated under the protection of a cultivation pool, so that the influence of pests on the yolk fries of the paramisgurnus dabryanus is reduced; when theyolk fries of the paramisgurnus dabryanus grows to 2-2.5cm, the eating of the pests can be resisted; and after the yolk fries of the paramisgurnus dabryanus grows to a total length of 4-5cm through three-grade cultivation, the yolk fries of the paramisgurnus dabryanus can instead take the pests as baits, so that the survival rate of the paramisgurnus dabryanus is greatly improved.

A high-density cultivation method for paramisgurnus dabryanus first-filial generation relates to the technical field of aquiculture and comprises the following steps: preparing a loach pond, cultivating a fingerling and controlling diseases. The high-density cultivation method has the benefits that the production resource is fully utilized, special equipment is not required, raising pollution is avoided, the raising technology is standard and normalized, feeding is scientific and normalized, the technology is novel, the aquiculture quality is improved to a great extent, and accordingly, the profit is increased.

The invention discloses an identification method for hybrid species of paramisgurnus dabryanus and misgurnus anguillicaudatus. The method comprises the following steps: (1) carrying out mutual interspecies amplification by virtue of two types of microsatellite markers of the paramisgurnus dabryanus and the misgurnus anguillicaudatus to find out specific markers of the paramisgurnus dabryanus and the misgurnus anguillicaudatus; (2) respectively carrying out PCR amplification on the three markers in the step (1) by taking the DNA of the misgurnus anguillicaudatus to be identified as a template so as to obtain three types of PCR amplification products; and (3) respectively carrying out gel electrophoresis analysis on the three types of PCR amplification products, and when an electrophoretogram of the PCR amplification products shows that a tested sample has three belts marked by Pda71, Mac287 and Mac375, determining the tested sample as a hybrid species misgurnus anguillicaudatus. The identification method disclosed by the invention not only can quickly and effectively identify the hybrid species of the paramisgurnus dabryanus and the misgurnus anguillicaudatus, but also can identify whether the male parent and the female parent of the hybrid species misgurnus anguillicaudatus are the misgurnus anguillicaudatus or the paramisgurnus dabryanus.

A high protein feed for the reproduction period of paramisgurnus dabryanus relates to the technical field of aquaculture and is prepared from the following raw materials (by weight): 3-5 parts of wheat powder, 2-4 parts of corn flour, 3-5 parts of wheat bran, 2-4 parts of rice bran, 3-5 parts of bean dregs, 6-8 parts of silkworm chrysalis meal, 3-5 parts of tenebrio molitor powder, 5-7 parts of dried-powder of housefly larvae, 3-5 parts of amino acid, 5-7 parts of vitamin mixed component, 2-4 parts of aliphatic acid, 2-4 parts of an adhesive and 6-8 parts of mineral powder. The invention has the following beneficial effects: raw materials are rich and cheap; the manufacture technology is simple; by introducing animal materials into common cereal materials, content of protein is increased; and micro-materials are added, immunity is raised, and ingestion requirement of paramisgurnus dabryanus in the reproduction period is guaranteed.

The invention discloses a paramisgurnus-dabryanus developmental-phase compounded feed, and relates to the technical field of aquaculture. The paramisgurnus-dabryanus developmental-phase compounded feed is prepared from 20-30 parts of wheat powder, 15-25 parts of corn flour, 10-20 parts of wheat bran, 8-12 parts of rice bran, 6-8 parts of bean dregs, 10-20 parts of egg white, 2-4 parts of gelatin, 6-8 parts of pig bone powder, 3-5 parts of monocalcium phosphate, 3-5 parts of blood powder, 2-4 parts of shrimp shell powder and 6-8 parts of mineral substance powder. The paramisgurnus-dabryanus developmental-phase compounded feed has the advantages that the raw materials are rich and cheap, and the preparing technology is simple and convenient; as microelements in the common cereal raw materials and the egg white are added, the content of protein is increased, and the requirements of paramisgurnus dabryanus in the development phase for feed are met.

The invention discloses special compound feed for paramisgurnus dabryanus and a preparation method thereof. The special compound feed for the paramisgurnus dabryanus comprises the following componentsin parts by weight: 15 to 25 parts of fish meal, 10 to 20 parts of fish steak powder, 5 to 10 parts of corn protein powder, 20 to 40 parts of soybean meal, 4 to 8 parts of rapeseed meal, 14 to 22 parts of high gluten flour, 2 to 8 parts of soybean oil, 1 to 2 parts of calcium dihydrogen phosphate, 0.1 to 0.5 part of choline chloride, and 1 to 2 parts of premixture. The special compound feed for the paramisgurnus dabryanus is prepared by adopting various components such as fish meal, fish steak powder, corn protein powder, soybean meal, rapeseed meal, high gluten flour, soybean oil, calcium dihydrogen phosphate, choline chloride and premixture, so that the nutritional demand of the paramisgurnus dabryanus can be better satisfied, the digestion and utilization rate when the special compoundfeed is used for feeding the paramisgurnus dabryanus is high, the growth rate and feed conversion rate of the paramisgurnus dabryanus can be increased, the growth performance of the paramisgurnus dabryanus can be improved, and the breeding efficiency can be improved.

The invention discloses a breeding method of albino paramisgurnus dabryanus capable of being inherited stably. The method comprises the steps as follows: a TYR (tyrosinase) gene target site is designed on a TYR gene sequence of paramisgurnus dabryanus, then an upstream primer and a downstream primer matched with the upstream primer are designed, PCR (polymerase chain reaction) amplification is performed with framework plasmid as a template, in-vitro transcription and purification are performed, and gRNA is obtained; Cas9 mRNA is obtained from linear Cas9 plasmid as a template after in-vitro transcription and purification; micro-injection is performed on fertilized eggs of the paramisgurnus dabryanus, the fertilized eggs are then incubated, gene knock-out fish is subjected to hybridization, and the albino paramisgurnus dabryanus capable of being inherited stably is obtained.

Provided is selective breeding technology of yellow paramisgurnus dabryanus. The selective breeding technology comprises following steps: 1, reserving parent fish; 2, selecting reserved parent fish; 3, selecting and breeding parents; 4, breeding and sieving parent fish; 5, providing fine parent fish; 6, performing artificial reproduction; 7, breeding seedlings; 8, authenticating and sieving germplasm; 9, breeding adult fish. The selective breeding technology of yellow paramisgurnus dabryanus provides a set of operation technique process suitable to hatching of local seedlings and offers a scientific basis to the selective breeding system of yellow paramisgurnus dabryanus.

The invention discloses a microsatellite-based paramisgurnus dabryanus family identification method and application thereof. The microsatellite-based paramisgurnus dabryanus family identification method comprises the following steps: acquiring paramisgurnus dabryanus full-sib families; extracting genomic DNA from parents and offsprings; screening polymorphic microsatellite markers, and synthesizing primers; performing fluorescence PCR amplification on DNA samples of the parents and offsprings; performing genotypic analysis on the parents and offsprings; and the like. The number of selected microsatellite site alleles is large, the polymorphism is high, and a PCR product is stable and reliable. When the microsatellite sites are used for identifying the 17 full-sib families, the probabilities of finding true male and female parents for 340 offspring individuals are 95.9% and 97.9% respectively, and the overall success rate is as high as 95.6%; by the microsatellite-based paramisgurnus dabryanus family identification method, requirements on identification of a parental sibship and family management in genetic paramisgurnus dabryanus breeding can be met, and a strong technical supportis provided for molecular breeding of paramisgurnus dabryanus.

The invention discloses a breeding method for paramisgurnus dabryanus parent loaches for hybridization, and relates to the technical field of aquiculture. The method comprises the following steps: selection of parent loaches; time selection; pond condition creation; stocking density controlling; fodder feeding; prevention and cure of diseases. The breeding method has the benefits that the breeding method fully utilizes production resource, dispenses with special equipment, has no breeding pollution, is regulated and standard in breeding technology, scientific and regulated in feeding stuff throw, and novel in polyculture technology, and improves quality of aquiculture in great extent, so as to improve profit.

The invention discloses a method for obtaining high-proportion female loaches through interspecies cross. The method includes the steps that female tetraploid loaches and male paramisgurnus dabryanus serve as parents for cross; oxytocic medicine is injected into abdominal cavities of the female tetraploid loaches and abdominal cavities of the male paramisgurnus dabryanus, after hasten parturition is carried out for 12-16 hours, testes of the male paramisgurnus dabryanus and egg grains of the female tetraploid loaches are collected, the testes and preserving liquid are grinded and mixed evenly and then mixed with the egg grains evenly, fertilized eggs are obtained and then incubated, and the high-proportion female loaches are obtained from the hybrid loach fries obtained through breeding and incubation. In triploid hybrid loach groups obtained according to the method, the proportion of the female hybrid loaches reaches up to 78.76% to 90.30%, the female loaches are fertile and can produce two kinds of eggs of different ploidies, and male loaches are infertile.

The invention discloses a paramisgurnus dabryanus breeding method in autumn and winter, wherein three kinds of spawning induction drugs are compounded to achieve optimal spawning induction effect, andneedle injection mode is used to quickly realize the estrus of parent dabryanus so as to obtain high-quality fertilized eggs, during the hatching process of the fertilized eggs, (microtube oxygen increasing) hatching pond is used for hatching with still water, so that early egg membrane breaking caused by hatching with flow water, which influences the hatching rate, is avoided, the constant temperature water and the electrochemical water are used as hatching water before hatching, the electrochemical water with proper concentration purifies the impurities in the water through physical modes of electric flocculation and ionization, bacteria and parasites are killed by generated strong oxidizing substances instead of using chemicals, which provides an ideal sterile environment for the hatching of the fertilized eggs, and greatly reduces the cost for sterilization and disinfection of the water in the hatching process, the breeding method has the advantages of simple process, strong operability and low cost, production of two seasons in one year can be achieved, seedlings can be effectively supplied, and economic benefits of farmers are increased.

A method for culturing paramisgurnus dabryanus in a pond relates to the technical field of aquiculture and comprises the steps of determining the specification of a pond, preventing escape and enemy, obtaining a water source, ensuring the water quality of the pond, stocking loach species, feeding with a fodder, regulating daily management, controlling diseases and fishing timely. The method has the benefits that the production resource is fully utilized, special equipment is not required, raising pollution is avoided, the raising technology is standard and normalized, feeding is scientific and normalized, the technology is novel, the aquiculture quality is improved to a great extent, and accordingly, the profit is increased.

The present invention discloses a paramisgurnus dabryanus astaxanthin compound feed. The feed consists of the following raw materials in parts by weight: 180-250 parts of fish meal, 200-250 parts of peeled soybean meal, 130-180 parts of corn protein powder, 30-80 parts of corn oil, 50-100 parts of wheat bran, 80-150 parts of flour, 100-180 parts of dextrin, 10-30 parts of monocalcium phosphate, 5-15 parts of a vitamin premix, 5-15 parts of a mineral premix, 1-5 parts of lysine, 2-8 parts of methionine, 2-8 parts of choline chloride and 0.05-0.2 part of astaxanthin. The result shows that the content of proteins is significantly higher than that of control groups when the astaxanthin is 50-200 mg/kg. The range of addition amount of the astaxanthin can effectively promote the growth of paramisgurnus dabryanus, and improve the feed efficiency and improve the body components, wherein the best addition amount is 100 mg/kg. The weight gain rate and feed efficiency are taken as evaluation indexes, and regression equation predicts that the addition amount of astaxanthin in the compound feed is 100.00-151.06 mg/kg. With the increase of the addition amount of astaxanthin, the content of proteins among the paramisgurnus dabryanus components increases first and then gets steady. The paramisgurnus dabryanus astaxanthin compound fee can effectively improve the quality of paramisgurnus dabryanus.

The invention discloses and relates to a loach and paramisgurnus dabryanus hybrid breeding method and belongs to the technical field of aquaculture. The loach and paramisgurnus dabryanus hybrid breeding method comprises the following steps of parent breeding, pond management, artificial insemination and hatching, and seedling cultivation. The loach and paramisgurnus dabryanus hybrid breeding method can improve the survival rate of loach seedlings; a first-filial generation with Taiwanese paramisgurnus dabryanus as male parent and domestic green loach as the female parent is high in fertility rate, hatching rate and survival rate, quick in growth and similar to the domestic green loach in body form, color and taste.

The invention discloses anti-season feeding equipment for Paramisgurnus dabryanus. The anti-season feeding equipment comprises a greenhouse, a base arranged in the greenhouse and a cultivation pond arranged on the base and is characterized in that the bottom of the base is provided with a thermal insulation layer, a liquid guide channel communicated with an external liquid supply device is arranged in the base, the two opposite outer lateral walls of the cultivation pond are provided with movable blocks which can slide along the length direction of the cultivation pond, the movable blocks areconnected with the cultivation pond through slide drive mechanisms, feed distribution components which can rotate along the horizontal direction is rotatably connected to the tops of the movable blocks and located above the cultivation pond, a feed storage hopper for supplying feed for the feed distribution component is arranged on one side of the top of each feed distribution component, each feeddistribution component comprises a rectangular mounting plate and a lateral column, and a feed conveying mechanism is arranged at the top of each mounting plate along the length direction. The anti-season feeding equipment has the advantages that the Paramisgurnus dabryanus is cultivated in the greenhouse, the water temperature in the cultivation pond can be controlled conveniently, a good anti-season cultivation effect is achieved, automatic and uniform feeding is achieved, and manpower can be saved effectively.

A paramisgurnus dabryanus fry breeding technology comprises the following steps of stocking in a parent pool: firstly building a special parent pool, performing thorough disinfection before stocking, then, selecting young paramisgurnus dabryanus of which the size is basically the same, and putting the selected young paramisgurnus dabryanus into the parent pool for breeding. Strengthening breeding: feeding artificial compound feed on the basis of provision of natural fish bait, adjusting the usage amount of the feed according to the conditions at intervals, frequently observing the change of water quality during the breeding process, and changing water one time per week in general. Then parents are selected for induced spawning, and suitable physique and maturity of parent fishes are selected. Then artificial propagation, natural spawning and fertilizing are performed, and then fertilized ova are collected and hatched into fries in circular hatching channel slow flowing water. Stocking fries can be selected for breeding according to the pool area, the number of the fries, estimated output and the like.