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Cold shock induction method for paramisgurnus dabryanus androgenesis haploid

A technology of paraloach and male nucleus development, which is applied in fish farming, application, climate change adaptation, etc., can solve problems such as destruction, cytoplasmic damage, and abnormal development, so as to avoid operating procedures, huge economic potential, and easy The effect of the operation

Inactive Publication Date: 2014-03-26
DALIAN OCEAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this inactivation method is simple and feasible, when the nucleus of the egg is destroyed by radiation, the cytoplasm and other organelles will also be damaged to varying degrees, resulting in the failure of normal development after fertilization.

Method used

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  • Cold shock induction method for paramisgurnus dabryanus androgenesis haploid
  • Cold shock induction method for paramisgurnus dabryanus androgenesis haploid

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Embodiment Construction

[0012] The specific implementation manners of the embodiments of the present invention will be described below in conjunction with the accompanying drawings, as figure 1 , figure 2 Shown: a kind of method for cold shock induction androgenetic haploid of Paraloach maxima, carry out according to the following steps:

[0013] Take the well-developed male and female paraloach, induce labor with the traditional method of inducing labor, the water temperature is 23±1°C, and the effect time is 10-12 hours. After confirming ovulation, gently press the abdomen to collect the female paraloach ovum, and the semen of the male paraloach with capillary collection, the gained semen is diluted 80-12 times with Kurokura's solution solution for stand-by, and its dilution factor is the best with 100 times; the preparation method of Kurokura's solution solution is prior art, i.e. Dissolve 750 mg of NaCl, 20 mg of CaCl2, 20 mg of NaHCO3, 20 mg of KCl in 100 ml of distilled water;

[0014] Acco...

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Abstract

The invention discloses a cold shock induction method for a paramisgurnus dabryanus androgenesis haploid. The cold shock induction method for the paramisgurnus dabryanus androgenesis haploid is characterized by comprising the following steps that parturition hastening is conducted on paramisgurnus dabryanus good in gonad development, semen of male paramisgurnus dabryanus and eggs of female paramisgurnus dabryanus are taken respectively, and the obtained semen is diluted by 80-120 times through the Kurokura's solution; the diluted semen and eggs are mixed for forming germ cells, the germ cells are put into water at the temperature of 2.5-3.5 DEG C, cold shock processing is conducted for 50-70 minutes, and then the germ cells are put into the water at normal temperature for incubation. The cold shock induction method for the paramisgurnus dabryanus androgenesis haploid does not need egg inactivation, and can produce the paramisgurnus dabryanus androgenesis haploid rapidly and efficiently.

Description

technical field [0001] The invention relates to a method for fish androgenic haploids, in particular to a method for cold shock inducing androgenic haploids of Paraloach grandis. Background technique [0002] Androgenesis refers to the reproductive mode of using sperm to produce individuals with only paternal genetic material. It can be used for the establishment of pure lines, and at the same time, it can freeze the sperm of endangered species, which has special significance for the protection of species. In addition, in aquaculture, because different sexes of many important economic fishes often show different production performances, such as differences in body shape and body color, growth speed, sexual maturity time, etc. For example, after feeding for 137 days, the weight growth rate of male Mozambique tilapia is 1.74 times faster than that of female fish; another example is feeding for 2 years, the weight of females is generally 100-200 g, and the weight of males is g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 李雅娟姜志强周贺王玉生李佳奇
Owner DALIAN OCEAN UNIV
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