Microsatellite-based paramisgurnus dabryanus family identification method and application thereof

A technique for the identification of Paraloach and its identification method, which is applied in the field of molecular marker assistance, and can solve problems such as family identification of Paraloach with no microsatellite markers, achieve high polymorphism, high identification accuracy, and speed up the breeding process Effect

Active Publication Date: 2018-11-20
天津市水产研究所
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Problems solved by technology

At present, there is no report on the application of microsa...

Method used

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  • Microsatellite-based paramisgurnus dabryanus family identification method and application thereof
  • Microsatellite-based paramisgurnus dabryanus family identification method and application thereof
  • Microsatellite-based paramisgurnus dabryanus family identification method and application thereof

Examples

Experimental program
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Embodiment 1

[0041] 1) Establishment of a full-sib family of Paraloach chinensis

[0042]Seventeen male and female individuals were selected from three wild populations of Dahuangbao Wetland, Chaobai New River and Tuanbo Reservoir in Tianjin, and 17 male and female individuals were artificially induced to reproduce. The ratio of male to female was 1:1, and 17 full-sib families were established. The fin ray tissues of the parents of each family were clipped and placed in absolute ethanol, and the family information was recorded and stored at -20°C for later use. 17 families were placed in 17 plastic water tanks for separate breeding. After the fry hatched for 15 days, 20 fish were randomly selected from each family and fixed with absolute ethanol as samples for paternity testing.

[0043] 2) Extraction of parental and offspring genomic DNA

[0044] Cut about 0.2 g of fin ray tissue from each parent, and take the whole fish from the offspring, put them in a 2 mL centrifuge tube, cut the tis...

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Abstract

The invention discloses a microsatellite-based paramisgurnus dabryanus family identification method and application thereof. The microsatellite-based paramisgurnus dabryanus family identification method comprises the following steps: acquiring paramisgurnus dabryanus full-sib families; extracting genomic DNA from parents and offsprings; screening polymorphic microsatellite markers, and synthesizing primers; performing fluorescence PCR amplification on DNA samples of the parents and offsprings; performing genotypic analysis on the parents and offsprings; and the like. The number of selected microsatellite site alleles is large, the polymorphism is high, and a PCR product is stable and reliable. When the microsatellite sites are used for identifying the 17 full-sib families, the probabilities of finding true male and female parents for 340 offspring individuals are 95.9% and 97.9% respectively, and the overall success rate is as high as 95.6%; by the microsatellite-based paramisgurnus dabryanus family identification method, requirements on identification of a parental sibship and family management in genetic paramisgurnus dabryanus breeding can be met, and a strong technical supportis provided for molecular breeding of paramisgurnus dabryanus.

Description

technical field [0001] The invention relates to a molecular marker auxiliary technology for fish genetic breeding, in particular to a method for identifying the family line of Paraloach chinensis by using fluorescent marker microsatellite markers. Background technique [0002] Paramisgurnus dabryanus, commonly known as yellow loach, is a small freshwater economic fish belonging to Cypriniformes, Cobitidae, and Paramisgurnus. Distributed in my country's Heilongjiang, the middle and lower reaches of the Liaohe River, the Haihe River system, the Yangtze River, the Qiantang River and Taiwan and other water systems. Because of its high edible value and medicinal value, it is very popular in domestic and foreign markets, and it is one of the important aquatic products exported to South Korea and Japan in my country. [0003] Paraloach has strong adaptability to the environment, and it can be cultured in ponds, paddy fields, cages and factories, and is an ideal breeding object. A...

Claims

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Application Information

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IPC IPC(8): C12Q1/6858C12Q1/6806
CPCC12Q1/6806C12Q1/6858C12Q2531/113C12Q2565/125C12Q2563/107C12Q2545/101C12Q2521/537
Inventor 白晓慧罗璋杨华孟一耕王娜刘克明刘义马林
Owner 天津市水产研究所
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