Paramisgurnus dabryanus selective breeding method

Abstract

The invention discloses a paramisgurnus dabryanus selective breeding method. Concretely, the method comprises the following steps that (1) 12 microsatellite molecular markers relevant to the growth properties are provided; (2) a parent group 1 is selected for electronic marking and DNA (deoxyribonucleic acid) extraction; (3) the molecular markers are adopted for carrying out genotype screening on the parent group 1, and in addition, a parent group 2 is selected; (4) the molecular markers are adopted for carrying out genotype screening on the parent group 2, and parent groups 3 and 4 with different homozygous genotypes are respectively built; (5) the parent groups 3 and 4 are used as breeding groups to be respectively subjected to artificial reproduction to obtain parent groups 5 and 6; (6) hybridization is carried out between the parent groups 5 and 6, and in addition, scaled reproduction is carried out. Compared with a conventional breeding method, the method has the characteristics of high speed, stability and reliability, the practicability is high, the cost is low, the accuracy is high, the influence due to factors such as environment is avoided, the relying on the phenotypic character authentication can be avoided, the breeding years are shortened, the breeding efficiency is improved, and great application values are realized.

Description

technical field [0001] The invention belongs to the field of fish breeding, and in particular relates to a method for breeding fine varieties of Paraloach chinensis. Background technique [0002] Paraloach with large scales ( Paramisgurnus dabryanus ) belongs to Loachidae, Loach subfamily, and Paraloach. It is a small freshwater benthic fish distributed in Southeast Asia. It is mostly distributed in Sichuan, Zhejiang, Taiwan, Liaoning, Heilongjiang and other places in China. Body nearly cylindrical, head short. The mouth is inferior, horseshoe-shaped, with a small gap in the center of the lower lip. The nostrils are close to the eyes, there are no spines under the eyes, and the gill openings are small. The head has no scales, and the body scales are larger than those of loach. 5 pairs of barbels, including 2 pairs of snout, 1 pair of palps, 2 pairs of chin barbels, each of which is long, and the palps behind the mouth can reach the rear edge of the operculum. The eyes a...

AI Technical Summary

Problems solved by technology

However, the systematic genetic selection of Paraloach chinensis has not yet started at home and abroad, especially the molecular marker-assisted breeding. The lack of high-quality seed has seriously restricted the development of the aquaculture industry of Paraloach chinensis. Therefore, it is urgent to develop a The Breeding Method of Improved Variety of Paraloach

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