Preservation solution, activation solution and hatching method for Paramisgurnus dabryanus sperms

A technology of paraloach and preservation solution, which is applied in the preservation of human or animal bodies, climate change adaptation, animal husbandry, etc. It can solve the problems of reducing the fertilization rate of fish eggs, affecting sperm motility, and less semen, so as to improve fertilization The effect of rate and hatchability

Inactive Publication Date: 2018-02-23
FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, in the process of artificial reproduction, the semen of Paraloach maxima is difficult to squeeze out, and its semen is relatively small. Generally, it is necessary to kill the fish to extract the semen, cut it

Method used

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  • Preservation solution, activation solution and hatching method for Paramisgurnus dabryanus sperms

Examples

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Embodiment 1

[0024] 1. Materials

[0025] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0026] 2. Method

[0027] 2.1 Preparation of sperm preservation solution

[0028] By weight, add 0.5%-1.0% trisodium citrate, 1.0%-3.0% glucose, 0.1%-0.3% sodium bicarbonate, 0.02%-0.04% potassium chloride, 0.01%-0.02% VC and double distilled water Mix and place on a magnetic stirrer to stir for 3-5 minutes. After fully dissolving, adjust to volume and adjust the pH to 7.5-8.0.

[0029] 2.2 Collection of semen

[0030] Anesthetize the mature male loach with a fish stabilizer, dry the genital area of ​​the male fish with a towel, press the abdomen lightly, and collect the extruded semen with a straw.

[0031] 2.3 Semen preservation

[0032] The collected semen was added to the sperm preservation solution equivalent t...

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Abstract

The invention discloses a preservation solution, an activation solution and a hatching method for Paramisgurnus dabryanus sperms, belonging to the technical field of aquaculture. The sperm preservation solution comprises the following components: 0.5% to 1.0% of trisodium citrate, 1.0% to 3.0% of glucose, 0.1% to 0.3% of sodium bicarbonate, 0.02% to 0.04% of potassium chloride and 0.01% to 0.02% of VC; the activation solution comprises the following components: 1 to 2% of glucose and 0.35% of sodium chloride; and the hatching method comprises the following steps: activating sperms preserved with the sperm preservation solution by using the activation solution, allowing the sperms to combine with eggs, placing fertilized eggs on a mesh, and carrying out hatching in static water. Compared with the prior art, the invention has the following advantages: through innovation of the activation manner of the Paramisgurnus dabryanus sperms in a specific sperm preservation solution, the maximum vitality of the sperms can be guaranteed; the fertilization rate and the hatching rate of fish eggs are improved; and the fry breeding production is guided.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a sperm preservation solution, an activation solution and a hatching method of the paraloach. Background technique [0002] Loach is called "water ginseng" by people. It contains higher protein and amino acids needed by the human body than ordinary fish and meat, and is favored by consumers. Paraloach chinensis has become one of the key development objects of famous, special and high-quality aquatic products, and then artificial breeding and artificial breeding of Paraloach chinensis have been carried out all over the country. However, in the process of artificial reproduction, the semen of Paraloach maxima is difficult to squeeze out, and its semen is relatively small. Generally, it is necessary to kill the fish to extract the semen, cut it into pieces and grind it, and add a certain amount of sperm activation solution. If it is not handled properly, it will often affect Sp...

Claims

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Application Information

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IPC IPC(8): A01N1/02A01K61/17
CPCA01K61/17A01N1/0226Y02A40/81
Inventor 王永杰陈红莲潘庭双钟诗群程云生
Owner FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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