Breeding method of albino paramisgurnus dabryanus capable of being inherited stably
An albino technology of paraloach maxima, which is applied to the introduction of foreign genetic material using vectors, recombinant DNA technology, animal husbandry, etc., can solve the problems of supply and demand difficulties of albino fish species, and achieve the effect of high knockout efficiency and short cycle.
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[0030] The implementation and application of the present invention will be described below in conjunction with implementation cases.
[0031] 1. Extraction of total RNA from the skin of Paraloach grandiflora
[0032] Use TaKaRa's RNAiso Plus reagent to extract the total RNA of Paraloach macronata. The specific steps are as follows:
[0033] 1) RNA extraction: all utensils, surgical scissors, and tweezers should be treated with DEPC overnight; the water used is RNase-free Water purchased by TaKaRa; the centrifuge EP tube, each type of pipette tip is dedicated for RNA extraction or DEPC water Soak overnight and autoclave. Note: Wear a mask, change gloves, and avoid talking during the extraction of total RNA. Pre-cool the sampling instruments on ice before the experiment;
[0034] 2) Put to death the Paraloach, quickly separate the skin tissue, and take 30mg-50mg tissue sample in a 2mL centrifuge tube, place the centrifuge tube in an ice bath, add 1.5mL RNAiso Plus reagent beforehand, ...
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