A method to obtain a high proportion of male loach through interspecific hybridization
A high-proportion, male technology, applied in fish farming, application, climate change adaptation, etc., can solve problems such as human harm, and achieve the effect of simple method, fewer breeding generations, and strong repeatability
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Embodiment 1
[0019] First, 8 healthy and good wild female paraloach and 6 male tetraploid loach were obtained from Ezhou City, Hubei Province as parents (the ploidy of the parents was identified by flow cytometry) (see appendix figure 1 ), the ratio of male and female loach parents is 4:3. Parents were injected with the oxytocic drug ovulation-stimulating hormone No. 2 (LRH-A 2 ) and dioconone (DOM), the injection dose for female fish was LRH-A 2 35μg / kg+DOM4mg / kg, the dose of male fish is halved, and the injection method is a single intraperitoneal injection. After the injection of oxytocin drugs, the male and female fish were kept separately in different water tanks, and the water temperature was 24-26°C. 12-16 hours after the injection of oxytocic drugs, manually collect the testis, put it in a mortar, add a small amount of loach sperm preservation solution (preservation solution formula: sodium chloride 7.5g, potassium chloride 0.3g, calcium chloride 0.3g, glucose 3.0 g, distilled w...
Embodiment 2
[0024] 8 well-developed female paraloach and 3 male tetraploid loach were selected for hybridization, and 136 hybrid loach were randomly collected, and the ploidy was identified as triploid by flow cytometry. The sex of the hybrid loach was identified by means of appearance, internal anatomy, and gonad section observation. The identification results are shown in Table 2.
[0025] Table 2 The sex ratio of the hybrid progenies of Paraloach ♀×Tetraploid Loach ♂
[0026]
[0027] Can prove from embodiment 1, 2, the present invention carries out hybridization with female paraloach and male tetraploid loach as parent, obtains the triploid hybrid loach population of high proportion male, shows bigger growth advantage and very Poor fertility, its popularization will largely promote the development of loach breeding industry and create good economic benefits.
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