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A method to obtain a high proportion of male loach through interspecific hybridization

A high-proportion, male technology, applied in fish farming, application, climate change adaptation, etc., can solve problems such as human harm, and achieve the effect of simple method, fewer breeding generations, and strong repeatability

Inactive Publication Date: 2016-01-20
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the unisexual fish population obtained by this method has great food safety problems, and a large amount of sex hormones will flow into the human body through feeding reversal fish, causing great harm to humans

Method used

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  • A method to obtain a high proportion of male loach through interspecific hybridization
  • A method to obtain a high proportion of male loach through interspecific hybridization
  • A method to obtain a high proportion of male loach through interspecific hybridization

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Experimental program
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Effect test

Embodiment 1

[0019] First, 8 healthy and good wild female paraloach and 6 male tetraploid loach were obtained from Ezhou City, Hubei Province as parents (the ploidy of the parents was identified by flow cytometry) (see appendix figure 1 ), the ratio of male and female loach parents is 4:3. Parents were injected with the oxytocic drug ovulation-stimulating hormone No. 2 (LRH-A 2 ) and dioconone (DOM), the injection dose for female fish was LRH-A 2 35μg / kg+DOM4mg / kg, the dose of male fish is halved, and the injection method is a single intraperitoneal injection. After the injection of oxytocin drugs, the male and female fish were kept separately in different water tanks, and the water temperature was 24-26°C. 12-16 hours after the injection of oxytocic drugs, manually collect the testis, put it in a mortar, add a small amount of loach sperm preservation solution (preservation solution formula: sodium chloride 7.5g, potassium chloride 0.3g, calcium chloride 0.3g, glucose 3.0 g, distilled w...

Embodiment 2

[0024] 8 well-developed female paraloach and 3 male tetraploid loach were selected for hybridization, and 136 hybrid loach were randomly collected, and the ploidy was identified as triploid by flow cytometry. The sex of the hybrid loach was identified by means of appearance, internal anatomy, and gonad section observation. The identification results are shown in Table 2.

[0025] Table 2 The sex ratio of the hybrid progenies of Paraloach ♀×Tetraploid Loach ♂

[0026]

[0027] Can prove from embodiment 1, 2, the present invention carries out hybridization with female paraloach and male tetraploid loach as parent, obtains the triploid hybrid loach population of high proportion male, shows bigger growth advantage and very Poor fertility, its popularization will largely promote the development of loach breeding industry and create good economic benefits.

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Abstract

The invention discloses a method for obtaining high-proportion male loaches through interspecies cross. The method includes the steps that female paramisgurnus dabryanus and male tetraploid loaches serve as parents for cross; oxytocic medicine is injected into abdominal cavities of the female paramisgurnus dabryanus and abdominal cavities of the male tetraploid loaches, after hasten parturition is carried out for 12-16 hours, testes of the male tetraploid loaches and egg grains of the female paramisgurnus dabryanus are collected, the testes and preserving liquid are grinded and mixed evenly and then mixed with the egg grains evenly, fertilized eggs are obtained and then incubated, and the high-proportion male loaches are obtained from the hybrid loach fries obtained through breeding and incubation. In triploid hybrid loach groups obtained through the method, the proportion of the male hybrid loaches reaches up to 87.64% to 98.53%, and the ovaries of the female loaches are in a hypoplasia state.

Description

technical field [0001] The invention relates to a fish parthenogenesis method, more specifically, it relates to a method for obtaining a high proportion of male loach through interspecific hybridization. Background technique [0002] Numerous studies have shown that single-sex groups (all-female or all-male groups) of fish have clear advantages in terms of growth rate or maintenance of a healthy ecological environment. At present, there are mainly the following methods for producing single-sex populations of fish at home and abroad: direct feeding of sex hormones, three-line matching method, gynogenesis and interspecific hybridization. Direct feeding of sex hormones to achieve sex reversal of fish was often used in the past, and all-female or all-male populations can often be obtained relatively smoothly by feeding high-concentration female or male hormones for a long time. However, the unisexual fish populations obtained by this method have great food safety problems, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 曹小娟王卫民黄松钱高坚田先畅
Owner HUAZHONG AGRI UNIV
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