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A method to obtain a high proportion of female loach through interspecific hybridization

A high-ratio, female technology, applied in fish farming, application, climate change adaptation, etc., can solve human hazards and other problems, and achieve the effect of simple method, strong repeatability, and few breeding generations

Inactive Publication Date: 2016-05-18
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the unisexual fish population obtained by this method has great food safety problems, and a large amount of sex hormones will flow into the human body through feeding reversal fish, causing great harm to humans

Method used

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  • A method to obtain a high proportion of female loach through interspecific hybridization
  • A method to obtain a high proportion of female loach through interspecific hybridization
  • A method to obtain a high proportion of female loach through interspecific hybridization

Examples

Experimental program
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Effect test

Embodiment 1

[0020] First, 8 wild female tetraploid loaches and 6 male paraloids with good health were obtained from Ezhou City, Hubei Province as parents (using flow cytometry to identify the ploidy of the parents) (see appendix figure 1 ), the ratio of male and female loach parents is about 4:3. Parents were injected with the oxytocic drug ovulation-stimulating hormone No. 2 (LRH-A 2 ) and dioconone (DOM), the injection dose for female fish was LRH-A 2 35μg / kg+DOM4mg / kg, the dose of male fish is halved, and the injection method is a single intraperitoneal injection. After the injection of oxytocin drugs, the male and female fish were kept separately in different water tanks, and the water temperature was 24-26°C. 12-16 hours after the injection of oxytocic drugs, manually collect the testis, put it in a mortar, add a small amount of loach sperm preservation solution (preservation solution formula: sodium chloride 7.5g, potassium chloride 0.3g, calcium chloride 0.3g, glucose 3.0 g, di...

Embodiment 2

[0029] 8 well-developed female tetraploid loach were selected for hybridization with 3 male paraloach, and 134 hybrid loach were randomly collected, and the ploidy was identified as triploid by flow cytometry. The sex of the hybrid loach was identified by means of appearance, internal anatomy, and gonad section observation. The identification results are shown in Table 3.

[0030] Table 3 The sex ratio of the hybrid offspring of tetraploid loach ♀ × paraloach ♂

[0031]

[0032] As can be seen from Examples 1 and 2, the present invention uses the female tetraploid loach and the male paraloach as parents to carry out hybridization to obtain a high-ratio female triploid hybrid loach population, and the female fish can simultaneously produce two kinds of Eggs of different ploidy (haploid and triploid), male fish are sterile, showing greater growth advantages and poor fertility, and its promotion will greatly promote the development of loach breeding industry, creating Very go...

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Abstract

The invention discloses a method for obtaining high-proportion female loaches through interspecies cross. The method includes the steps that female tetraploid loaches and male paramisgurnus dabryanus serve as parents for cross; oxytocic medicine is injected into abdominal cavities of the female tetraploid loaches and abdominal cavities of the male paramisgurnus dabryanus, after hasten parturition is carried out for 12-16 hours, testes of the male paramisgurnus dabryanus and egg grains of the female tetraploid loaches are collected, the testes and preserving liquid are grinded and mixed evenly and then mixed with the egg grains evenly, fertilized eggs are obtained and then incubated, and the high-proportion female loaches are obtained from the hybrid loach fries obtained through breeding and incubation. In triploid hybrid loach groups obtained according to the method, the proportion of the female hybrid loaches reaches up to 78.76% to 90.30%, the female loaches are fertile and can produce two kinds of eggs of different ploidies, and male loaches are infertile.

Description

technical field [0001] The invention relates to a fish parthenogenesis method, more specifically, it relates to a method for obtaining a high proportion of female loach through interspecific hybridization. Background technique [0002] Numerous studies have shown that single-sex groups (all-female or all-male groups) of fish have clear advantages in terms of growth rate or maintenance of a healthy ecological environment. At present, there are mainly the following methods for producing single-sex populations of fish at home and abroad: direct feeding of sex hormones, three-line matching method, gynogenesis and interspecific hybridization. Direct feeding of sex hormones to achieve sex reversal of fish was often used in the past, and all-female or all-male populations can often be obtained relatively smoothly by feeding high-concentration female or male hormones for a long time. However, the unisexual fish populations obtained by this method have great food safety problems, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 曹小娟王卫民黄松钱田先畅高坚
Owner HUAZHONG AGRI UNIV
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