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Method for obtaining high-proportion female loaches through interspecies cross

A high-proportion, female technology, applied in fish farming, application, climate change adaptation, etc., can solve problems such as human harm, and achieve the effect of simple method, strong repeatability, and fewer breeding generations

Inactive Publication Date: 2014-06-25
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the unisexual fish population obtained by this method has great food safety problems, and a large amount of sex hormones will flow into the human body through feeding reversal fish, causing great harm to humans

Method used

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  • Method for obtaining high-proportion female loaches through interspecies cross
  • Method for obtaining high-proportion female loaches through interspecies cross
  • Method for obtaining high-proportion female loaches through interspecies cross

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Firstly, 8 wild female tetraploid loach with good health and 6 male paraloach large scale were obtained from Ezhou City, Hubei Province as parents (use flow cytometry to identify the parental ploidy) (see attached figure 1 ), the ratio of female and male loach parents is about 4:3. Injection of ovulation-stimulating hormone 2 (LRH-A 2 ) And dioxone (DOM), the injection dose of female fish is LRH-A 2 35μg / kg+DOM4mg / kg, the male fish dose is halved, and the injection method is a single intraperitoneal injection. After the oxytocin drug injection, the male and female fish were kept separately in different tanks at a temperature of 24-26°C. After 12-16 hours of injecting the oxytocin drug, the testes were collected manually, placed in a mortar, and a small amount of loach sperm preservation solution was added (preservation solution formula: sodium chloride 7.5g, potassium chloride 0.3g, calcium chloride 0.3g, glucose 3.0 g, double distilled water to 1000ml) for full grindin...

Embodiment 2

[0029] A well-developed 8-tail female tetraploid loach was selected for crossbreeding with 3 male large scale paraloach, 134 hybrid loach were randomly collected, and the ploidy was identified as triploid by flow cytometry. The sex of hybrid loach was identified by methods such as appearance observation, internal dissection and gonadal section observation. The identification results are shown in Table 3.

[0030] Table 3 The sex ratio of the hybrid offspring of tetraploid loach♀×Parachis davidiana ♂

[0031]

[0032] It can be seen from Examples 1 and 2 that in the present invention, the female tetraploid loach and the male Paraloach large scale are used as parents to cross, and a high proportion of female triploid hybrid loach population is obtained, and the female fish can produce two kinds at the same time. Eggs of different ploidy (haploid and triploid) and male fish are sterile, showing greater growth advantages and poor fertility. Their promotion will greatly promote the deve...

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Abstract

The invention discloses a method for obtaining high-proportion female loaches through interspecies cross. The method includes the steps that female tetraploid loaches and male paramisgurnus dabryanus serve as parents for cross; oxytocic medicine is injected into abdominal cavities of the female tetraploid loaches and abdominal cavities of the male paramisgurnus dabryanus, after hasten parturition is carried out for 12-16 hours, testes of the male paramisgurnus dabryanus and egg grains of the female tetraploid loaches are collected, the testes and preserving liquid are grinded and mixed evenly and then mixed with the egg grains evenly, fertilized eggs are obtained and then incubated, and the high-proportion female loaches are obtained from the hybrid loach fries obtained through breeding and incubation. In triploid hybrid loach groups obtained according to the method, the proportion of the female hybrid loaches reaches up to 78.76% to 90.30%, the female loaches are fertile and can produce two kinds of eggs of different ploidies, and male loaches are infertile.

Description

Technical field [0001] The present invention relates to a method for single-sex breeding of fish, more specifically, it relates to a method for obtaining a high proportion of female loach through interspecific hybridization. Background technique [0002] Many studies have shown that fish unisex groups (all-female or all-male groups) have obvious advantages in terms of growth rate or maintaining a healthy ecological environment. At present, there are mainly the following methods for producing single-sex populations of fish at home and abroad: direct feeding of sex hormones, three-line matching method, gynogenesis and interspecific hybridization. Direct feeding of sex hormones to achieve the reversal of the sex of fish is often used in the past. By feeding high concentrations of female or male hormones for a long time, it is often possible to obtain all-female or all-male populations smoothly. However, the single-sex group of fish obtained by this method has a great food safety pr...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 曹小娟王卫民黄松钱田先畅高坚
Owner HUAZHONG AGRI UNIV
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