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Identification method for hybrid species of paramisgurnus dabryanus and misgurnus anguillicaudatus

A technology of Paraloach and hybrid species, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem of identification of hybrid species, cannot be used to distinguish Paraloach and loach hybrids, Limited performance and other issues

Inactive Publication Date: 2016-05-04
刘海金
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Although the prior art with the Chinese Patent No. "201410775974.7" disclosed a germplasm identification primer and identification method for loach and Paraloach chinensis on April 8, 2015, this method can only be used to identify pure-bred loach and purebred Paraloach, but cannot be used to identify hybrids of Paraloach and loach
Therefore, the performance of its method is limited and cannot yet solve the identification problem of hybrid species

Method used

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  • Identification method for hybrid species of paramisgurnus dabryanus and misgurnus anguillicaudatus
  • Identification method for hybrid species of paramisgurnus dabryanus and misgurnus anguillicaudatus
  • Identification method for hybrid species of paramisgurnus dabryanus and misgurnus anguillicaudatus

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Embodiment Construction

[0026] A sort of The hybrid of paraloach and loach The identification method includes the following steps:

[0027] (1) Through mutual interspecific amplification of two microsatellite markers of Paraloach and Loach, specific markers of Paraloach and Loach were found. Among them, the reciprocal interspecific amplification through the two microsatellite markers of Paraloach and Loach includes the interspecific amplification of the Paraloach sample and the interspecific amplification of the Paraloach sample. The two specific methods of interspecies amplification are as follows:

[0028] The interspecies amplification of the loach sample with the marker of Paraloach chinensis, the specific amplification method is: extract the DNA of the multi-tailed loach, and select 44 microsatellite markers of the Paraloach chinensis, and then carry out inoculation with the DNA of each loach. During the PCR amplification, a specific marker that is not present in the pure loach can be obtained...

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Abstract

The invention discloses an identification method for hybrid species of paramisgurnus dabryanus and misgurnus anguillicaudatus. The method comprises the following steps: (1) carrying out mutual interspecies amplification by virtue of two types of microsatellite markers of the paramisgurnus dabryanus and the misgurnus anguillicaudatus to find out specific markers of the paramisgurnus dabryanus and the misgurnus anguillicaudatus; (2) respectively carrying out PCR amplification on the three markers in the step (1) by taking the DNA of the misgurnus anguillicaudatus to be identified as a template so as to obtain three types of PCR amplification products; and (3) respectively carrying out gel electrophoresis analysis on the three types of PCR amplification products, and when an electrophoretogram of the PCR amplification products shows that a tested sample has three belts marked by Pda71, Mac287 and Mac375, determining the tested sample as a hybrid species misgurnus anguillicaudatus. The identification method disclosed by the invention not only can quickly and effectively identify the hybrid species of the paramisgurnus dabryanus and the misgurnus anguillicaudatus, but also can identify whether the male parent and the female parent of the hybrid species misgurnus anguillicaudatus are the misgurnus anguillicaudatus or the paramisgurnus dabryanus.

Description

technical field [0001] The invention relates to a method for identifying hybrids in the field of aquaculture, in particular to a method for identifying hybrids of Paraloach and loach by using molecular markers. Background technique [0002] Paramisgurnus dabryanus belongs to the order Cypriniformes, the family Cobitidae, and the genus Paramisgurnus. Loach (Misgurnus anguillicaudatus) belongs to Cypriniformes (Cypriniformes), loach family (Cobitidae), loach genus (Misgurnus). Loach is distributed in freshwater waters all over the country except the Qinghai-Tibet Plateau, and is an important economic fish for freshwater aquaculture in my country. Loach is known as "ginseng in water", has various medicinal values, and is traditionally consumed by the public. However, due to the similar shape of loach and paraloach, non-professionals generally cannot accurately distinguish them. In recent years, there has been an upsurge of cultivating loach in China. In addition to cultivatin...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 刘海金刘永富
Owner 刘海金
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