Application of a skin color-related SNP marker in silky chicken genotyping

A genotyping and labeling technology, used in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of unsuitability for large-scale sample detection, complicated SSCP operation, and high detection cost, and achieve rapid and accurate individualization. The effect of screening, low cost and easy operation
CN107988376BActive Publication Date: 2020-08-07LESHAN NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
LESHAN NORMAL UNIV
Publication Date
2020-08-07

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Abstract

The invention provides an application of a skin color-associated SNP marker in genotyping of black-bone chickens. The SNP marker is located at 2228 bp upstream of a transcription initiation site in apromoter region of a TYR gene, and a base with polymorphism is adenine A or thymine T. The SNP genotype detection primer has high specificity, single detection band and accurate typing results. In combination with a provided detection method, the whole operation process is simpler and more efficient, and the cost is lower. Rapid and accurate individual screening is achieved, and a basis for breeding development and protection of black-bone chicken varieties is provided.
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Description

technical field

[0001] The invention belongs to the field of molecular biology detection, and in particular relates to the application of a color-related SNP marker in silky chicken genotyping. Background technique

[0002] Chicken skin color is a very important breed characteristic as a quality trait. Chicken skin color has an important economic use due to different market consumption needs. The skin color of poultry is mainly divided into three types: white, yellow and black, and the black skin is represented by silky chicken.

[0003] Black-bone chicken has special nourishing and medicinal value, and its most notable feature is that it contains a large amount of melanin in the body. As an important active substance, black-bone chicken melanin has the functions of scavenging free radicals, anti-oxidation, delaying aging and improving body immunity. Studies have shown that black-bone chicken melanin can enrich trace elements such as Cu, Mn, Fe, Co and Ni, and the above e...

Claims

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