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Application of a skin color-related SNP marker in silky chicken genotyping

A genotyping and labeling technology, used in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of unsuitability for large-scale sample detection, complicated SSCP operation, and high detection cost, and achieve rapid and accurate individualization. The effect of screening, low cost and easy operation

Active Publication Date: 2020-08-07
LESHAN NORMAL UNIV
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AI Technical Summary

Problems solved by technology

However, these methods have certain disadvantages. Among them, SSCP is complex and time-consuming, and is not suitable for the detection of a large number of samples.
The cost of sequencing and TaqMan probe genotyping is too high, and RFLP is not suitable for a large number of samples

Method used

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  • Application of a skin color-related SNP marker in silky chicken genotyping
  • Application of a skin color-related SNP marker in silky chicken genotyping
  • Application of a skin color-related SNP marker in silky chicken genotyping

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] Materials and Methods

[0034] Experimental animals and sample collection:

[0035] A total of 188 healthy adult Muchuan black-bone chickens were selected in this experiment, and the experimental objects were all under the unified feeding and management conditions of Muchuan County Heifenghuang Black-bone Chicken Industry Co., Ltd. Blood collection: Use a disposable blood collection needle to collect blood through the wing vein into a vacuum blood collection tube containing sodium citrate anticoagulant, and mix well. At the same time, the skin color (black, light black, white) of silky chickens was recorded.

[0036] equipment:

[0037] Low-temperature high-speed centrifuge, ultra-micro-volume spectrophotometer, adjustable micro-pipette, gel imaging system, electronic balance, high-pressure sterilizer, constant temperature water bath, electrophoresis apparatus, gradient PCR instrument.

[0038] Genomic DNA Extraction

[0039] 1. Put 20ul anticoagulated whole blood i...

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Abstract

The invention provides an application of a skin color-associated SNP marker in genotyping of black-bone chickens. The SNP marker is located at 2228 bp upstream of a transcription initiation site in apromoter region of a TYR gene, and a base with polymorphism is adenine A or thymine T. The SNP genotype detection primer has high specificity, single detection band and accurate typing results. In combination with a provided detection method, the whole operation process is simpler and more efficient, and the cost is lower. Rapid and accurate individual screening is achieved, and a basis for breeding development and protection of black-bone chicken varieties is provided.

Description

technical field [0001] The invention belongs to the field of molecular biology detection, and in particular relates to the application of a color-related SNP marker in silky chicken genotyping. Background technique [0002] Chicken skin color is a very important breed characteristic as a quality trait. Chicken skin color has an important economic use due to different market consumption needs. The skin color of poultry is mainly divided into three types: white, yellow and black, and the black skin is represented by silky chicken. [0003] Black-bone chicken has special nourishing and medicinal value, and its most notable feature is that it contains a large amount of melanin in the body. As an important active substance, black-bone chicken melanin has the functions of scavenging free radicals, anti-oxidation, delaying aging and improving body immunity. Studies have shown that black-bone chicken melanin can enrich trace elements such as Cu, Mn, Fe, Co and Ni, and the above e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 喻世刚王钢廖娟
Owner LESHAN NORMAL UNIV
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