Reconstructed ovum of albinism model pig and construction method thereof, and construction method of model pig

A construction method and technology of albinism, applied in the field of genetic engineering, can solve the problems of unfavorable experimental operation, unfavorable observation, non-genetically modified albino pigs and other problems

Active Publication Date: 2015-01-07
广州中科飞豚生物科技有限责任公司
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Problems solved by technology

At present, albino mice have become commonly used experimental animals in biomedical research, and are widely used in burn, skin toxicity, allergy testing, etc., but so far there are no reports of genetically modified albino pigs
At present, the skin and hair of wild-type minipigs are mostly black or colored, which is not conducive to observation in research, while wild-type white pigs are mostly seen in large-scale pig breeds for commercial meat. Their large size is not conducive to experimental operations and is not suitable for experimental animals.

Method used

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  • Reconstructed ovum of albinism model pig and construction method thereof, and construction method of model pig
  • Reconstructed ovum of albinism model pig and construction method thereof, and construction method of model pig
  • Reconstructed ovum of albinism model pig and construction method thereof, and construction method of model pig

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Embodiment Construction

[0042] The reconstituted eggs of albinism model pigs and their construction methods and the construction methods of model pigs will be further described in detail below mainly in conjunction with the accompanying drawings and specific examples.

[0043] In one embodiment, the method for constructing an albinism model pig includes the process of constructing a model pig's reconstituted egg, activating the reconstituted egg, and introducing the activated reconstituted egg into a surrogate sow for growth.

[0044] Such as figure 1 As shown, the construction method of the reconstructed egg of the albinism model pig of the present embodiment comprises the following steps:

[0045] Step S110, for the positive strand and the complementary strand of the first exon of the tyrosinase gene of the porcine species satisfy G(N) 19 The sequence part of the NGG sequence pattern designs gRNA recognition sequences respectively, which are respectively recorded as the first gRNA recognition sequ...

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Abstract

The invention relates to a reconstructed ovum of an albinism model pig and a construction method thereof, and a construction method of the model pig. According to the construction method of the reconstructed ovum, tyrosinase is knocked out to obtain the reconstructed ovum of the albinism model pig. The combination of a CRISPP / Cas9 gene knock-out technique and a somatic nucleus transplantation technique proves that the method for constructing the gene modified pig has high feasibility. The obtained TYR gene knock-out pig has the typical characteristics of albinism, can provide a reliable animal model for researching human albinism, can be applied to toxicity and anaphylaxis detection and many other aspects, and is hopeful to become a standardized experimental animal for albinism-like mice.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a reconstituted egg of an albinism model pig, a construction method thereof, and a construction method of a model pig. Background technique [0002] The research and treatment of human diseases are inseparable from experimental animal models. Rodents are quite different from humans in terms of gene expression and various physiological indicators. Although non-human primates are the closest experimental animals to humans, they are expensive and have ethical obstacles. Pigs are similar to humans in terms of body size, physiological conditions, organ development, and disease progression; therefore, pigs are considered a suitable experimental animal model. Genetically modified pig models are expected to play an important role in the study of pathogenesis and treatment strategies of human diseases. Among the current methods for making genetically modified animals, only the embryon...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N5/10A01K67/027
Inventor 赖良学信吉阁杨化强邹庆剑樊娜娜
Owner 广州中科飞豚生物科技有限责任公司
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