Primer, kit and detection method for detecting recessive white feather locus genotype of chicken

A recessive white feather, genotype technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as low feed conversion rate, poor meat quality, slow growth rate, etc. Simple, short typing time and low cost
CN103571963AActive Publication Date: 2014-02-12HENAN AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN AGRICULTURAL UNIVERSITY
Publication Date
2014-02-12

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Abstract

The invention discloses a primer, a kit and a detection method for detecting a recessive white feather locus genotype of a chicken, belonging to the technical field of biology. According to the invention, a specific primer P1 of an allelic gene c is designed according to the fact that 7.7kb insertion (ev1) exists in a fourth intron of a TYR gene, a specific primer P2 of the allelic gene c is designed according to the fact that 7.7kb insertion (ev1) does not exist in a fourth intron of a PMEL17 gene, a primer P3 is a common downstream primer of the primer P1 and the primer P2, a whole genome of a to-be-detected chicken containing the TYR gene is taken as a template, the primers P1, P2 and P3 are put in the same PCR (Polymerase Chain Reaction), an amplified fragment of the PCR is subjected to agarose gel electrophoresis after once PCR, and the recessive white feather locus genotype of the TYR gene of the chicken is identified according to the result of the agarose gel electrophoresis. The method greatly improves the genotype judging efficiency and accuracy, the method is simple, the genotype judging time is short, no sequencing or restriction enzyme is needed, no special instrument is required, the cost is low, and the method is easy to popularize.
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Description

technical field

[0001] The invention specifically relates to a primer for detecting the genotype of a chicken recessive white feather locus, a kit containing the primer and a detection method, and belongs to the field of biotechnology. Background technique

[0002] With the improvement of living standards, people's consumption concept has changed from the quantity type to the quality type. People's requirements for chicken meat quality are also getting higher and higher. Local chickens in my country have the characteristics of good meat quality, but their growth rate is slow and their feed conversion rate is low. Although foreign fast large broilers grow fast and have a high feed conversion rate, their meat quality is poor. In order to solve this problem, crossing foreign fast large broilers with domestic local high-quality broilers can not only improve the meat quality of foreign fast large broilers but also accelerate the growth rate and production performance of local h...

Claims

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