Primer group for culturing homozygous black feather chicken and method for culturing homozygous black feather chicken
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A breeding method and primer set technology, applied in the field of poultry breeding, can solve the problems of white feathered individuals, unfavorable breeding, inconvenient chicken breeding work, etc., and achieve the effects of ensuring uniformity and protecting breeding materials.
Pending Publication Date: 2018-01-05
SHANDONG AGRICULTURAL UNIVERSITY +1
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The inheritance of chicken feather color is relatively complicated, and it takes a long process to purify feather color. The use of dominant white feathers as breeding materials is obviously unfavorable for the breeding of high-quality local chicken breeds in China.
Recessive white feathers are recessive to the colored feathers of Chinese local chicken breeds, and the feather color of offspring hybridized wi
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Embodiment 1
[0040] A primer set for breeding homozygous black feather chickens, the primer set comprises a first primer set, a second primer set and a third primer set, wherein:
[0041] The forward primer of the first primer set is 5'-GCCGCCATCCTCAAGAAC-3', and the reverse primer is 5'-GAGAGGGAGGACACGATGGA-3';
[0042] The forward primer of the second primer set is 5'-GTTGCCTCTGGCTCTATT-3', and the reverse primer is 5'-TTTCTGTGAGTAAGGGTTG'-3;
[0043] The forward primer of the third primer set is 5'-TTGTTGGAGGCTGGGTAT-3' and the reverse primer is 5'-ATGGGCTTGCTTGAGGTA-3'.
Embodiment 2
[0045] A method for cultivating homozygous black-feathered chickens utilizing the primer set described in Example 1, comprising the following steps:
[0046] A. Select the existing Laiwu black chickens whose phenotype is black feathers, shank and beak black, and white skin individuals, and determine them as chickens to be identified.
[0047] B, use the phenol-chloroform extraction method to extract the genomic DNA of the chicken to be identified, and use the first primer set, the second primer set and the third primer set to amplify respectively to obtain the amplified product; the primer design of this example is as follows As shown in Table 1;
[0048] Table 1. Primer set sequence list
[0049]
[0050] When using the first primer set to amplify, the amplification system is: the total volume is 20 μL, including Tris-HCL3 3.5mM, (NH 4 ) 2 SO 4 8.0mM, MgCl 2 1.5mM, TWEEN-20 0.05%, dNTPs 0.2mM, forward primer and reverse primer 3.3ng / μl, Taq DNA polymerase 2.0 units ...
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Abstract
The invention provides a primer group for culturing homozygous black feather chicken and a method for culturing the homozygous black feather chicken by using the primer group. The primer group comprises a first primer group, a second primer group and a third primer group, wherein the first primer group is used for detecting whether an MC1R gene is mutated or not; the second primer group and the third primer group are used for detecting whether retrovirus is inserted into a TYR gene to cause a recessive white feather gene or not. The culturing method has the advantages that by using the first primer group, the second primer group and the third primer group, and simultaneously detecting the MC1R gene and the TYR gene, the EE (corresponding to the MC1R gene) and CC (corresponding to the TYR gene) gene type black feather chicken are screened out, and are homozygotes, and the recessive white feather gene is removed; the white feature individual will not occur in the filial generation when the primer group is matched with other systems, and the uniformity of the black feather phenotype is ensured; the breeding material of the breeding enterprise can be well protected on the basis of ensuring the product uniformity of the breeding enterprise.
Description
technical field [0001] The invention belongs to the technical field of poultry breeding, and in particular relates to a primer set for breeding homozygous black-feather chickens and a method for breeding homozygous black-feather chickens. Background technique [0002] There are many poultry genetic resources in my country. The 2011 edition of "China Livestock and Poultry Genetic Resources-Poultry Chronicle" included 189, including 107 chickens. Laiwu black chicken is mainly distributed in Laiwu area of Shandong Province. Now it has become a local breed with obvious characteristics through artificial breeding and natural selection. Laiwu black chicken has a black body coat, dense growth, metallic luster, a single red crown, and a straight head and neck. , tall tail feathers, back like "U" shape, with black hair, black legs, black feet, black mouth, black eyes, red crown, white ears, five black, one white and one red; good at climbing, easy to fly, good physique Robust, str...
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