Primer group for culturing homozygous black feather chicken and method for culturing homozygous black feather chicken

Abstract

The invention provides a primer group for culturing homozygous black feather chicken and a method for culturing the homozygous black feather chicken by using the primer group. The primer group comprises a first primer group, a second primer group and a third primer group, wherein the first primer group is used for detecting whether an MC1R gene is mutated or not; the second primer group and the third primer group are used for detecting whether retrovirus is inserted into a TYR gene to cause a recessive white feather gene or not. The culturing method has the advantages that by using the first primer group, the second primer group and the third primer group, and simultaneously detecting the MC1R gene and the TYR gene, the EE (corresponding to the MC1R gene) and CC (corresponding to the TYR gene) gene type black feather chicken are screened out, and are homozygotes, and the recessive white feather gene is removed; the white feature individual will not occur in the filial generation when the primer group is matched with other systems, and the uniformity of the black feather phenotype is ensured; the breeding material of the breeding enterprise can be well protected on the basis of ensuring the product uniformity of the breeding enterprise.

Description

technical field [0001] The invention belongs to the technical field of poultry breeding, and in particular relates to a primer set for breeding homozygous black-feather chickens and a method for breeding homozygous black-feather chickens. Background technique [0002] There are many poultry genetic resources in my country. The 2011 edition of "China Livestock and Poultry Genetic Resources-Poultry Chronicle" included 189, including 107 chickens. Laiwu black chicken is mainly distributed in Laiwu area of ​​Shandong Province. Now it has become a local breed with obvious characteristics through artificial breeding and natural selection. Laiwu black chicken has a black body coat, dense growth, metallic luster, a single red crown, and a straight head and neck. , tall tail feathers, back like "U" shape, with black hair, black legs, black feet, black mouth, black eyes, red crown, white ears, five black, one white and one red; good at climbing, easy to fly, good physique Robust, str...

AI Technical Summary

Problems solved by technology

The inheritance of chicken feather color is relatively complicated, and it takes a long process to purify feather color. The use of dominant white feathers as breeding materials is obviously unfavorable for the breeding of high-quality local chicken breeds in China.

Recessive white feathers are recessive to the colored feathers of Chinese local chicken breeds, and the feather color of offspring hybridized with Chinese local chickens is the same as that of Chinese local chickens, but due to the existence of recessive white feather genes, further hybrid offspring may appear white feathers individual

And because the recessive white feather cannot be directly reflected in the phenotype, it cannot be manually screened out through the phenotype, which brings great inconvenience to the breeding work of chicken breeds

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