Preparation method of pegylation modified hyperbranched poly(ethylene imine) coated nano-gold particles

A polyethyleneimine and PEGylation technology, which is applied in the directions of X-ray contrast agent preparation, bulk delivery, etc., can solve the problem that the preparation method has not yet been reported.

Inactive Publication Date: 2013-08-14
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Retrieval of relevant literature and patents found that there is no relevant report on the preparation method of polyethyleneimine-coated gold nanoparticles with different molar ratios modified by mPEG (polyethylene glycol monomethyl ether)

Method used

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  • Preparation method of pegylation modified hyperbranched poly(ethylene imine) coated nano-gold particles
  • Preparation method of pegylation modified hyperbranched poly(ethylene imine) coated nano-gold particles
  • Preparation method of pegylation modified hyperbranched poly(ethylene imine) coated nano-gold particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Dissolve 60.00 mg of mPEG-COOH with 5 mL of dimethyl sulfoxide, then add 57.51 mg of EDC·HCl (dissolved in 5 mL of dimethyl sulfoxide) and stir at room temperature for 30 min, then add 34.53 mg of NHS ( Dissolve with 5mL of dimethyl sulfoxide) and continue to stir at room temperature for 3h.

[0046] (2) Dissolve 25.00 mg of PEI with 10 mL of dimethyl sulfoxide, then add the solution prepared in step (1) dropwise while stirring, react for 3 days, and then dialyze the reaction product in water for 3 days (9x2L), Freeze-dried to obtain white powder solid PEI-mPEG.

[0047] (3) Dissolve 5.00 mg of solid PEI-mPEG prepared in step (2) in water, and take 269.1 μL of 10 mg / mL HAuCl 4 Mix the aqueous solution and stir vigorously at room temperature for 30min, the solution turns light yellow, then add 1.237mL of 1mg / mL NaBH 4 solution, the solution turned into wine red instantly, and continued to stir vigorously at room temperature for 2h.

[0048] (4) Add 27.32 μL of tri...

Embodiment 2

[0051] (1) Dissolve 60.00 mg of mPEG-COOH with 5 mL of dimethyl sulfoxide, then add 57.51 mg of EDC·HCl (dissolved in 5 mL of dimethyl sulfoxide) and stir at room temperature for 30 min, then add 34.53 mg of NHS ( Dissolve with 5mL of dimethyl sulfoxide) and continue to stir at room temperature for 3h.

[0052] (2) Dissolve 25.00 mg of PEI with 10 mL of dimethyl sulfoxide, then add the solution prepared in step (1) dropwise while stirring, react for 3 days, and then dialyze the reaction product in water for 3 days (9x2L), Freeze-dried to obtain white powder solid PEI-mPEG.

[0053] (3) Dissolve 5.00 mg of solid PEI-mPEG prepared in step (2) in water, and take 538.2 μL of 10 mg / mL HAuCl 4 Mix the aqueous solution and stir vigorously at room temperature for 30min, the solution turns light yellow, then add 2.474mL of 1mg / mL NaBH 4 solution, the solution turned into wine red instantly, and continued to stir vigorously at room temperature for 2h.

[0054] (4) Add 27.32 μL of tri...

Embodiment 3

[0057] (1) Dissolve 60.00 mg of mPEG-COOH with 5 mL of dimethyl sulfoxide, then add 57.51 mg of EDC·HCl (dissolved in 5 mL of dimethyl sulfoxide) and stir at room temperature for 30 min, then add 34.53 mg of NHS ( Dissolve with 5mL of dimethyl sulfoxide) and continue to stir at room temperature for 3h.

[0058] (2) Dissolve 25.00 mg of PEI with 10 mL of dimethyl sulfoxide, then add the solution prepared in step (1) dropwise while stirring, react for 3 days, and then dialyze the reaction product in water for 3 days (9x2L), Freeze-dried to obtain white powder solid PEI-mPEG.

[0059] (3) Dissolve 5.00 mg of solid PEI-mPEG prepared in step (2) in water, and take 807.3 μL of 10 mg / mL HAuCl 4 Mix the aqueous solution and stir vigorously at room temperature for 30min, the solution turns light yellow, then add 3.711mL of 1mg / mL NaBH 4 solution, the solution turned dark red instantly, and continued to stir vigorously at room temperature for 2h.

[0060] (4) Add 27.32 μL of triethyl...

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Abstract

The invention relates to a preparation method of pegylation modified hyperbranched poly(ethylene imine) (PEI) coated nano-gold particles, which comprises the following steps: modifying PEI by using mPEG (polyethylene glycol)-COOH, and sequentially carrying out dialysis and freeze-drying on the obtained product so as to obtain PEI-mPEG; taking the solid, dissolving the solid by using water, adding a HAuCl4 solution into the dissolved solid, stirring the obtained product, adding a NaBH4 solution into the obtained product, and carrying out reaction on the obtained mixture at room temperature; and adding triethylamine and acetic anhydride into the obtained object, and after the reaction is completed, carrying out dialysis and freezing on the obtained product so as to obtain pegylation modified hyperbranched polymine coated nano-gold particles. According to the invention, the cheap and easily-obtained PEI is taken as a carrier, so that the cost of materials is reduced; the surface of PEI is modified by using mPEG-COOH, so that the biocompatibility of materials and the colloidal stability of nano-gold particles are improved, and the nano-gold particles are successfully applied to vivo CT (computed tomography) imaging. The method disclosed by the invention is simple in design, mild in reaction conditions and easy to operate, and has an industrialized implementation prospect.

Description

technical field [0001] The invention belongs to the field of preparation of CT contrast agents, in particular to a method for preparing PEGylated hyperbranched polyethyleneimine-wrapped gold nanoparticles. Background technique [0002] Gold nanoparticles (Au NPs) have been widely used in biomedicine due to their unique physicochemical properties, especially in the fields of biosensing, disease diagnosis, photothermal therapy, and drug and gene delivery. Among the numerous examples of polymer-assisted synthesis of Au NPs, nanoparticles synthesized with hyperbranched polyethyleneimine molecules (PEI) as templates or stabilizers have attracted considerable attention. This is mainly because PEI is a hyperbranched macromolecule with a large number of amino groups on its outer surface, and the amino groups are easy to be further modified, allowing researchers to easily modify various biological functional groups on its surface. For example, PEI has been used as a very effective n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/04A61K9/16
Inventor 史向阳周本青彭琛张贵祥郑林丰
Owner DONGHUA UNIV
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