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Preparation method of tree-like macromolecular and stable ultra-small ferroferric oxide/gold nano-flowers

A technology of dendritic macromolecules and ferric tetroxide, which is used in medical preparations containing active ingredients, general/multifunctional contrast agents, preparations for in vivo tests, etc., can solve the problem of not finding ultra-small ferric tetroxide / gold nanoflowers, etc., to achieve high practical value, simple preparation method, and small cytotoxic effect

Inactive Publication Date: 2018-09-11
DONGHUA UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The results of domestic and foreign literature and patent searches on tumor multimodal imaging and combined therapy nanomaterials show that the preparation of ultra-small ferric oxide / gold nanoflowers with stable dendrimers and their role in tumor MR / Reports on the application of CT / PA multimodal imaging and combined photothermal therapy / radiotherapy

Method used

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  • Preparation method of tree-like macromolecular and stable ultra-small ferroferric oxide/gold nano-flowers
  • Preparation method of tree-like macromolecular and stable ultra-small ferroferric oxide/gold nano-flowers
  • Preparation method of tree-like macromolecular and stable ultra-small ferroferric oxide/gold nano-flowers

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Experimental program
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Effect test

Embodiment 1

[0045] (1) 0.648g anhydrous ferric chloride FeCl 3 Dissolve in 40mL of diethylene glycol, stir at room temperature until completely dissolved, add 0.471g of sodium citrate, stir at 80°C for 1h until the sodium citrate is completely dissolved, then add 1.312g of anhydrous sodium acetate and continue stirring until the solution is clear, then Transfer the reaction solution to a 50mL autoclave, react at 200°C for 4h, cool to room temperature, transfer the product to a 50mL centrifuge tube and centrifuge at 8500rpm for 15min, discard the supernatant, redissolve with absolute ethanol, and centrifuge at 8500rpm for 15min. Repeat the operation 3 times, and then dry the precipitate at 60°C to obtain ultra-small ferric oxide nanoparticles Fe 3 o 4 NPs; 56mg of the nanoparticles were dispersed in 10mL of DMSO, 144mg of EDC and 70mg of NHS were dispersed in 1mL of DMSO solution, and then both were added to the ultrasmall Fe 3 o 4 NPs DMSO dispersion, stirred at room temperature for 3 ...

Embodiment 2

[0050]The present invention uses transmission electron microscopy (TEM), ultraviolet-visible spectrophotometer (UV-Vis), cell viability analysis (CCK8 test), and in vivo and in vitro MR / CT / PA multimodal imaging and photothermal therapy / radiotherapy combined treatment characterization Dendrimers-stabilized ultra-small ferric oxide / gold nanoflower G5-Fe with multimodal imaging and combined therapy prepared by the present invention 3 o 4 -AuNFs.

[0051] TEM test:

[0052] In order to characterize the morphology and size of the prepared nanoparticles, the ultra-small Fe prepared in Example 1 of the present invention were respectively taken 3 o 4 NPs, G5-Au NPs and G5-Fe 3 o 4 - 1 mg of Au NFs was dissolved in 1 mL of ultrapure water to prepare a nanoparticle suspension. Take 5 μL each ultra-small Fe 3 o 4 NPs, G5-Au NPs and G5-Fe 3 o 4 -The suspension of Au NFs was dropped on the surface of the copper grid, air-dried and used for TEM test, the results are as follows f...

Embodiment 3

[0058] Material T 1 Weighted MR Imaging Test:

[0059] To evaluate G5-Fe 3 o 4 - Imaging effect of Au NFs as MRI contrast agents, combining materials with pure ultra-small Fe 3 o 4 r of NPs 1 Relaxation rate for comparison, r 1 The relaxation rate is the longitudinal relaxation time per molar concentration of iron, which can be obtained by T at different concentrations 1 The inverse of the relaxation time was calculated by fitting. Get the ultra-small Fe prepared in Example 1 3 o 4 NPs and G5-Fe 3 o 4 -AuNFs were dissolved in ultrapure water, and the concentration of Fe in the solution was measured by ICP-OES test method, and then ultrapure water was used to prepare aqueous solutions (2mL) with Fe concentrations of 0.1, 0.2, 0.4, 0.8 and 1.6mM , to determine the T at different Fe concentrations 1 Weighted imaging (eg Figure 5 a) and T 1 relaxation time (as Figure 5 As shown in b, ultra-small Fe 3 o 4 NPs and G5-Fe 3 o 4 -T of Au NFs 1 Linear fitting graph...

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Abstract

The invention relates to a preparation method of tree-like macromolecular and stable ultra-small ferroferric oxide / gold nano-flowers. The preparation method comprises the following steps: mixing FeCl3, sodium citrate and anhydrous sodium acetate and reacting to obtain ultra-small Fe3O4NPs; carrying out activation treatment to obtain activated ultra-small Fe3O4NPs; enabling a G5 solution to react with a chloroauric acid solution to obtain G5-Au NPs; enabling the G5-Au NPs to react with the ultra-small Fe3O4NPs, so as to obtain G5-Fe3O4-Au NPs; enabling the chloroauric acid solution, an AgNO3 solution and an ascorbic acid AA solution to react, so as to obtain a G5-Fe3O4-AuNFs solution; carrying out acetylation treatment to obtain a product. Nanoparticles prepared by the preparation method have excellent biocompatibility and a good MR / CT / PA imaging function; the nanoparticles can be used for photothermal therapy of tumors; a photothermal therapy effect is improved through radiotherapy andthe nanoparticles can be used for a potential tumor multi-modal imaging contrast agent and combined therapy and have a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of multimodal imaging and combined therapy functional materials, and in particular relates to a preparation method of ultra-small iron ferric oxide / gold nanoflowers stabilized by dendrimers. Background technique [0002] Malignant tumors have the characteristics of fast growth, strong metastasis and high recurrence rate, and have become a major disease that threatens human health and life. Early diagnosis and early treatment are crucial to timely control the condition of tumor patients and prolong the survival time, so it has become a research hotspot for scientific researchers. Currently, common tumor diagnosis techniques include magnetic resonance imaging (MRI), computed tomography (CT) and photoacoustic imaging (PA). MRI imaging has the advantages of high spatial resolution and soft tissue resolution, but its sensitivity is low, and image acquisition and reconstruction processing time is long; CT imaging...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/04A61K49/18A61K49/22A61K41/00A61P35/00
CPCA61K41/0052A61K49/0002A61K49/04A61K49/1851A61K49/225A61P35/00
Inventor 沈明武陆诗怡李鑫彭琛史向阳
Owner DONGHUA UNIV
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