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Hyaluronic acid modified hectorite amide nanoparticle and preparation and application of hyaluronic acid modified hectorite amide nanoparticle

A modified technology of hectorite and hyaluronic acid, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of lack of internalization mechanism and achieve prolongation Circulation time, good targeting effect, effect of improved drug release profile

Inactive Publication Date: 2015-05-27
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, finding a suitable carrier is far from enough. Many anticancer drugs cannot pass through the cell membrane. Even if the drug carrier loads the drug to the surface of tumor cells, it still lacks an effective internalization mechanism.

Method used

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  • Hyaluronic acid modified hectorite amide nanoparticle and preparation and application of hyaluronic acid modified hectorite amide nanoparticle
  • Hyaluronic acid modified hectorite amide nanoparticle and preparation and application of hyaluronic acid modified hectorite amide nanoparticle
  • Hyaluronic acid modified hectorite amide nanoparticle and preparation and application of hyaluronic acid modified hectorite amide nanoparticle

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Experimental program
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Effect test

Embodiment 1

[0047] (1) Dissolve 518 mg of hyaluronic acid (HA) (purchased from Zhenjiang Dongyuan Biotechnology Co., Ltd.) in 20 mL of ultrapure water, and add 1 mL of 1-ethyl-(3-dimethyl Aminopropyl) carbodiimide (EDC) was stirred for 0.5 h, and then 1 mL of 10.1 mg / mL N-hydroxysuccinimide (NHS) was added to activate for 3 h;

[0048] (2) Take LAP powder and dissolve it in ultrapure water to obtain a dispersion with a concentration of 10 mg / mL. Take 40 μL (3-aminopropyl) dimethylethoxysilane and add 10 mL of LAP dispersion dropwise while shaking , then stand at 50°C for 16 hours, and dialyze the reaction product in distilled water for 72 hours with 8000-14000 dialysis bags to obtain aminated hectorite (LM) solution.

[0049] (3) Make the activated HA solution into a solution with a concentration of 2 mg / mL. Take 1 mL and add 1 mL of EDC with a concentration of 2.2 mg / mL, and then add 1 mL of NHS solution with a concentration of 1.32 mg / mL, and stir magnetically for 3 h. Slowly add 1 mL...

Embodiment 2

[0051] Take 1 mL of LM-HA aqueous solution with a concentration of 3 mg / mL, add 1 mL of DOX aqueous solution with a concentration of 1 mg / mL, and react with magnetic stirring for 24 h under the condition of avoiding light. After the reaction, transfer the solutions to 15 mL centrifuge tubes respectively, and Centrifuge (10 min) at a rotational speed of 5000 rpm, and wash the obtained precipitate three times with deionized water to obtain drug-loaded nanoparticles LM-HA / DOX.

Embodiment 3

[0053] Disperse LM-HA / DOX with buffer solutions of pH=7.4 and pH=5.4 respectively to form a solution with a concentration of 1mg / mL (the concentration of DOX in LM-HA / DOX), take 1mL into a dialysis bag for fixation, and place Place in a container containing 9mL of buffer solution of different pH and shake in a shaker at 37°C. The first 30min, 1h, 2h, 4h, 6h, 9h and 12h fixed-point sampling, after that, take a sample every 24h. Take 1mL of the liquid outside the dialysis bag each time, and then add 1mL of the corresponding buffer solution to the outside of the dialysis bag. The absorbance value at 480nm of the dialysate taken out was measured, and the release curve of DOX released from LM-HA / DOX under different pH conditions in vitro was calculated. ( Figure 4 )

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Abstract

The invention relates to a hyaluronic acid-modified amide hectorite nanoparticle and a preparation and an application of the hyaluronic acid modified hectorite amide nanoparticle. The hyaluronic acid modified hectorite amide nanoparticle is (3-amino propyl) ethoxydimethylsilane modified hectorite; and the mass fraction of the hyaluronic acid in the nanoparticle is 17.17%-19.09%. The preparation method comprises the following steps: modifying a hectorite surface with (3-amino propyl) ethoxydimethylsilane on to obtain hectorite amide; and through covalent action of amino and carboxyl, grafting the hectorite amide surface with hyaluronic acid, thus obtaining the hyaluronic acid modified hectorite amide nanoparticle. The stability and the biocompatibility of the nanoparticle are improved; meanwhile, the hyaluronic acid-modified hectorite amide nanoparticle has specific targeting action on cancer cells with high expression for a CD44 receptor, and can be applied to targeted delivery of anti-cancer drugs; and the hyaluronic acid-modified hectorite amide nanoparticle is simple in preparation method, mild in reaction condition and easy to operate, and has an industrial implementation prospect.

Description

technical field [0001] The invention belongs to the field of nano drug-carrying materials, and in particular relates to a hectorite amide nano particle modified by hyaluronic acid and its preparation and application. Background technique [0002] In the development of many human diseases, there is a major disease that seriously threatens human life and health-tumor. In the process of tumor chemotherapy, the key to the therapeutic effect is whether anticancer drugs can reach tumor cells and have a certain drug concentration, so as to inhibit the proliferation of cancer cells or even kill cancer cells. However, the therapeutic effect of a large number of drug molecules is greatly limited due to defects such as poor water solubility and low cell membrane permeability. Therefore, the key to solving this problem is to construct a drug delivery system with targeted drug delivery function. [0003] With the continuous development of nanotechnology and the emergence of a large num...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K47/46A61K47/36A61K31/704A61P35/00
Inventor 郭睿陈光祥
Owner DONGHUA UNIV
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