Preparation method for chiral dendrimers/gold nanoparticles modified by mPEG

A technology of dendrimer and gold nanoparticles, which is applied to preparations and pharmaceutical formulations for in vivo experiments, to achieve the effects of controllable size, simple method and high X-ray attenuation intensity

Inactive Publication Date: 2011-08-17

DONGHUA UNIV

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AI-Extracted Technical Summary

Problems solved by technology

[0005] At present, there is no relevant report on the preparation method of gold nanoparticles wrapped by po...

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Abstract

The invention relates to a preparation method for chiral dendrimers/gold nanoparticles modified by mPEG (Methoxy Polyethylene Glycol), comprising the steps of: (1) modifying the chiral dendrimers by using mPEG-MAL (Maleimide) and dialyzing, freezing and drying to obtain solids; (2) dissolving the solids with methanol or water, and adding a chloroauric acid solution and then a NaBH4 solution after mixing, reacting at a room temperature; and then adding triethylamine and acetic anhydride, dialyzing a reaction product after the reaction is ended and freezing to obtain a final product by. The invention has the advantages of increasing molar ratio of the gold to the chiral dendrimers and reducing cost of materials, and at the same time enhancing stability of the gold nanoparticles and being successfully applied in the mice's in-vivo imaging; and the method has the advantages of simpleness, mild reaction conditions and easy operation, and has the prospect for the implementation of industrialization.

Application Domain

In-vivo testing preparations

Technology Topic

ChemistryAcetic anhydride +11

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Examples

Experimental program(5)

Example Embodiment

[0045] Example 1

[0046] (1) Dissolve the G5PAMAM dendrimer with a dry weight of 30.00mg in 10mL of DMSO solution, and add mPEG-MAL with a dry weight of 46.41mg in 5mL of DMSO solution while stirring, of which mPEG-MAL/dendrimer (Molar ratio)=20:1, react for 72 hours, and then use cellulose dialysis membrane (MWCO=10000) to dialyze the reaction product in 4L PBS buffer solution for 3 times and 4L ultrapure water for 3 times. After 6 hours, finally freeze-dry the purified product to obtain G5.NH 2 -mPEG;

[0047] (2) Take step (1) and dissolve 5.00 mg of dried solid in methanol, and take 0.176 mL of 10 mg/mL HAuCl 4 Mix the methanol solution, stir at room temperature for 20 minutes, the solution turns light yellow, and then add 0.807mL of 1mg/mL NaBH 4 Solution (H 2 O: CH 3 OH (volume ratio)=2:1), the solution instantly turns deep red, stir for 2h at room temperature; add 7.53μL of triethylamine to the reaction solution of step (2), stir and react for 20min, then add to the reaction solution 4.92μL acetic anhydride, stirred at room temperature for 20h, and then the reaction product was dialyzed in 4L PBS buffer solution with cellulose dialysis membrane (MWCO=10000), dialyzed 3 times and ultrapure water 4L 3 times, each time 8h Dialysis for 3 days, and finally the purified product was freeze-dried to obtain mPEG-modified polyamide-amine dendrimer-encapsulated gold nanoparticles {(Au 0 ) 50 -G5.NHAc-mPEG}DENPs.

Example Embodiment

[0048] Example 2

[0049] (1) Dissolve G5PAMAM dendrimer with a dry weight of 20.00mg in 10mL DMSO solution, and add mPEG-MAL with a dry weight of 30.94mg dissolved in 5mL DMSO solution while stirring, where mPEG-MAL/dendrimer (Molar ratio)=20:1, react for 72 hours, and then use cellulose dialysis membrane (MWCO=10000) to dialyze the reaction product in 4L PBS buffer solution for 4 times and 4L ultrapure water for 2 times. After 8 hours, the purified product is freeze-dried to obtain G5.NH 2 -mPEG;

[0050] (2) Take step (1) and dissolve 5.00 mg of dried solid in water, and take 0.351 mL of 10 mg/mL HAuCl 4 The aqueous solution was mixed, stirred vigorously at room temperature for 30 min, the solution turned light yellow, and then 1.613 mL of 1 mg/mL NaBH was added 4 Solution (H 2 O: CH 3 OH (volume ratio)=2:1), the solution instantly turns dark red, stir vigorously for 2h at room temperature; add 7.53μL of triethylamine to the reaction solution of step (2), stir and react for 30min, then add to the reaction solution Add 4.92μL of acetic anhydride, stir the reaction vigorously at room temperature for 24h, and then use a cellulose dialysis membrane (MWCO=10000) to dialyze the reaction product twice in 4L of PBS buffer solution and 4 times in 4L of ultrapure water, each time After 6 hours of dialysis for 3 days, the purified product was finally freeze-dried to obtain mPEG-modified polyamide-amine dendrimer-encapsulated gold nanoparticles {(Au 0 ) 100 -G5.NHAc-mPEG}DENPs.

Example Embodiment

[0051] Example 3

[0052] (1) Dissolve dendrimers with a dry weight of 20.00 mg in 10 mL of DMSO solution, and add mPEG-MAL with a dry weight of 30.94 mg in 5 mL of DMSO solution while stirring, where mPEG-MAL/dendrimers ( Molar ratio)=20:1, react for 60 hours, and then use cellulose dialysis membrane (MWCO=10000) to dialyze the reaction product in 4L PBS buffer solution for 3 times and 4L ultrapure water for 3 times, each time 10h, finally freeze-dry the purified product to obtain G5.NH 2 -mPEG;

[0053] (2) Take step (1) and dissolve 5.00 mg of dried solid in methanol, and take 0.702 mL of 10 mg/mL HAuCl 4 The aqueous solution was mixed, stirred vigorously at room temperature for 30 min, the solution turned light yellow, and then 3.235 mL of 1 mg/mL NaBH was added 4 Solution (H 2 O: CH 3 OH (volume ratio)=2:1), the solution instantly turns dark red, stir vigorously for 2h at room temperature; add 7.53μL of triethylamine to the reaction solution of step (2), stir and react for 30min, then add to the reaction solution Add 4.92μL of acetic anhydride, stir the reaction vigorously at room temperature for 24h, and then use a cellulose dialysis membrane (MWCO=10000) to dialyze the reaction product 3 times in 4L PBS buffer solution and 3 times in 4L ultrapure water, each time 8h, dialyzed for 3 days, and finally the purified product was freeze-dried to obtain mPEG-modified polyamide-amine dendrimer-encapsulated gold nanoparticles {(Au 0 ) 200 -G5.NHAc-mPEG}DENPs.

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Description & Claims & Application Information

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the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

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Carboxymethyl chitosan quaternary ammonium salt as well as preparation and application thereof in preparing natural cosmetic antibacterial agent

ActiveCN102040670ASimple methodLow equipment requirementsCosmetic preparationsToilet preparationsChemistryMethyl group

Owner:FROG PRINCE (FUJIAN) BABY & CHILD CARE PROD CO LTD

Classification and recommendation of technical efficacy words

Simple method
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Preparation method for chiral dendrimers/gold nanoparticles modified by mPEG

A technology of dendrimer and gold nanoparticles, which is applied to preparations and pharmaceutical formulations for in vivo experiments, to achieve the effects of controllable size, simple method and high X-ray attenuation intensity

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Image

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

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