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Preparation method for chiral dendrimers/gold nanoparticles modified by mPEG

A technology of dendrimer and gold nanoparticles, which is applied to preparations and pharmaceutical formulations for in vivo experiments, to achieve the effects of controllable size, simple method and high X-ray attenuation intensity

Inactive Publication Date: 2011-08-17
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no relevant report on the preparation method of gold nanoparticles wrapped by polyamide-amine dendrimers with different molar ratios modified by mPEG (polyethylene glycol monomethyl ether)

Method used

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  • Preparation method for chiral dendrimers/gold nanoparticles modified by mPEG
  • Preparation method for chiral dendrimers/gold nanoparticles modified by mPEG
  • Preparation method for chiral dendrimers/gold nanoparticles modified by mPEG

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Dissolve the G5PAMAM dendrimer with a dry weight of 30.00mg in 10mL of DMSO solution, and add mPEG-MAL with a dry weight of 46.41mg in 5mL of DMSO solution while stirring, of which mPEG-MAL / dendrimer (Molar ratio)=20:1, react for 72 hours, and then use cellulose dialysis membrane (MWCO=10000) to dialyze the reaction product in 4L PBS buffer solution for 3 times and 4L ultrapure water for 3 times. After 6 hours, finally freeze-dry the purified product to obtain G5.NH 2 -mPEG;

[0047] (2) Take step (1) and dissolve 5.00 mg of dried solid in methanol, and take 0.176 mL of 10 mg / mL HAuCl 4 Mix the methanol solution, stir at room temperature for 20 minutes, the solution turns light yellow, and then add 0.807mL of 1mg / mL NaBH 4 Solution (H 2 O: CH 3 OH (volume ratio)=2:1), the solution instantly turns deep red, stir for 2h at room temperature; add 7.53μL of triethylamine to the reaction solution of step (2), stir and react for 20min, then add to the reaction solution 4.92μL...

Embodiment 2

[0049] (1) Dissolve G5PAMAM dendrimer with a dry weight of 20.00mg in 10mL DMSO solution, and add mPEG-MAL with a dry weight of 30.94mg dissolved in 5mL DMSO solution while stirring, where mPEG-MAL / dendrimer (Molar ratio)=20:1, react for 72 hours, and then use cellulose dialysis membrane (MWCO=10000) to dialyze the reaction product in 4L PBS buffer solution for 4 times and 4L ultrapure water for 2 times. After 8 hours, the purified product is freeze-dried to obtain G5.NH 2 -mPEG;

[0050] (2) Take step (1) and dissolve 5.00 mg of dried solid in water, and take 0.351 mL of 10 mg / mL HAuCl 4 The aqueous solution was mixed, stirred vigorously at room temperature for 30 min, the solution turned light yellow, and then 1.613 mL of 1 mg / mL NaBH was added 4 Solution (H 2 O: CH 3 OH (volume ratio)=2:1), the solution instantly turns dark red, stir vigorously for 2h at room temperature; add 7.53μL of triethylamine to the reaction solution of step (2), stir and react for 30min, then add to the...

Embodiment 3

[0052] (1) Dissolve dendrimers with a dry weight of 20.00 mg in 10 mL of DMSO solution, and add mPEG-MAL with a dry weight of 30.94 mg in 5 mL of DMSO solution while stirring, where mPEG-MAL / dendrimers ( Molar ratio)=20:1, react for 60 hours, and then use cellulose dialysis membrane (MWCO=10000) to dialyze the reaction product in 4L PBS buffer solution for 3 times and 4L ultrapure water for 3 times, each time 10h, finally freeze-dry the purified product to obtain G5.NH 2 -mPEG;

[0053] (2) Take step (1) and dissolve 5.00 mg of dried solid in methanol, and take 0.702 mL of 10 mg / mL HAuCl 4 The aqueous solution was mixed, stirred vigorously at room temperature for 30 min, the solution turned light yellow, and then 3.235 mL of 1 mg / mL NaBH was added 4 Solution (H 2 O: CH 3 OH (volume ratio)=2:1), the solution instantly turns dark red, stir vigorously for 2h at room temperature; add 7.53μL of triethylamine to the reaction solution of step (2), stir and react for 30min, then add to th...

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Abstract

The invention relates to a preparation method for chiral dendrimers / gold nanoparticles modified by mPEG (Methoxy Polyethylene Glycol), comprising the steps of: (1) modifying the chiral dendrimers by using mPEG-MAL (Maleimide) and dialyzing, freezing and drying to obtain solids; (2) dissolving the solids with methanol or water, and adding a chloroauric acid solution and then a NaBH4 solution after mixing, reacting at a room temperature; and then adding triethylamine and acetic anhydride, dialyzing a reaction product after the reaction is ended and freezing to obtain a final product by. The invention has the advantages of increasing molar ratio of the gold to the chiral dendrimers and reducing cost of materials, and at the same time enhancing stability of the gold nanoparticles and being successfully applied in the mice's in-vivo imaging; and the method has the advantages of simpleness, mild reaction conditions and easy operation, and has the prospect for the implementation of industrialization.

Description

Technical field [0001] The invention belongs to the preparation field of gold nanoparticles, and particularly relates to a method for preparing mPEG-modified dendrimer / gold nanoparticles. Background technique [0002] Precious metal nanoparticles (NPs), such as Au NPs and Ag NPs due to their unique optical, electronic and quantum size-related properties, have attracted considerable scientific and technical attention in the medical field, especially in biological transmission. In the field of infection, disease diagnosis, photothermal therapy, and drug and gene delivery. Among the numerous examples of Au DENPs synthesized with polymer-assisted synthesis, nanoparticles synthesized with dendrimers as templates or stabilizers have attracted a lot of attention. This is mainly due to the unique structure and properties of this new, highly branched and monodisperse synthetic dendrimer. The customizable surface chemical properties of dendrimers allow researchers to easily modify biolog...

Claims

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Application Information

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IPC IPC(8): C08L87/00C08G81/00C08K3/08A61K49/12
Inventor 史向阳彭琛
Owner DONGHUA UNIV
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