Detection method and kit for organophosphorus pesticides

A technology of organophosphorus pesticides and detection kits, applied in the field of analytical chemistry, can solve the problems of high-cost use conditions, protease variability and hydrolysis, etc., and achieve the effect of low cost and high stability

Active Publication Date: 2017-07-11
JIANGNAN UNIV
View PDF5 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can catalyze chemical reactions in a suitable environment, but due to the inherent characteristics of enzymes, its application is also subject to some restrictions, such as proteases are easy to denature and hydrolyze, and high cost and strict use conditions also limit their application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method and kit for organophosphorus pesticides
  • Detection method and kit for organophosphorus pesticides
  • Detection method and kit for organophosphorus pesticides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] (1) Preparation of gold-core platinum-shell nanoparticles: use sodium citrate reduction method to synthesize a gold nanoparticle solution with an average particle size of 15nm, then get 15ml of synthetic gold nanosolution (3nM), 0.112ml, 10mM hexachloroplatinic acid Potassium (K 2 PtCl 6 ) 9.328ml in ultrapure water and placed in a clean Erlenmeyer flask, stirred on a magnetic stirrer and heated to 80°C, then slowly added 0.56ml of 10mM reducing agent-ascorbic acid to the mixed solution, the color of the solution was determined by wine The red color gradually turns brown, keep the mixed solution at 80°C and continue to stir for 30 minutes to ensure the K in the solution 2 PtCl 6 It is completely reduced, that is, gold-core platinum-shell nanoparticles with a particle size of about 20nm are obtained, see figure 1 It is the TEM image of the prepared gold-core-platinum-shell nanoparticles.

[0057] (2) After diluting the gold core platinum shell nano solution obtained ...

Embodiment 2

[0062] (1) Preparation of gold-core platinum-shell nanoparticles: use sodium citrate reduction method to synthesize a gold nanoparticle solution with an average particle size of 20nm, then take 15ml of the synthesized gold nanosolution (3nM), 0.112ml, 10mM hexachloroplatinic acid Potassium (K 2 PtCl 6 ) 9.328ml in ultrapure water and placed in a clean Erlenmeyer flask, stirred on a magnetic stirrer and heated to 80°C, then slowly added 0.56ml of 10mM reducing agent-ascorbic acid to the mixed solution, the color of the solution was determined by wine The red color gradually turns brown, keep the mixed solution at 80°C and continue to stir for 30 minutes to ensure the K in the solution 2 PtCl 6 After being completely reduced, gold-core platinum-shell nanoparticles with a particle size of about 24nm are obtained.

[0063] (2) After diluting the gold core platinum shell nano solution obtained in step (1) 20 times, mix it with 0.002wt% polyacrylic acid solution and add it in the...

Embodiment 3

[0067] (1) Preparation of gold-core platinum-shell nanoparticles: use sodium citrate reduction method to synthesize a gold nanoparticle solution with an average particle size of 22nm, then take 15ml of the synthesized gold nanoparticle solution (3nM), 0.112ml, 10mM hexachloroplatinic acid Potassium (K 2 PtCl 6 ) 9.328ml in ultrapure water and placed in a clean Erlenmeyer flask, stirred on a magnetic stirrer and heated to 80°C, then slowly added 0.56ml of 10mM reducing agent-ascorbic acid to the mixed solution, the color of the solution was determined by wine The red color gradually turns brown, keep the mixed solution at 80°C and continue to stir for 30 minutes to ensure the K in the solution 2 PtCl 6 After being completely reduced, gold-core-platinum-shell nanoparticles with a particle size of about 26nm are obtained.

[0068] (2) After diluting the gold core platinum shell nano solution obtained in step (1) 20 times, mix with 0.005wt% polyacrylic acid solution and add in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
sizeaaaaaaaaaa
thicknessaaaaaaaaaa
Login to view more

Abstract

The invention discloses a detection method for organophosphorus pesticides. The detection method comprises the following steps: fully mixing a test sample which may contain organophosphorus pesticides (such as thimet, omethoate and rogor) with gold-core platinum-shell nanoparticles and polyacrylic acid in a phosphate buffer solution so as to form a first mixed system; and then adding a sodium citrate buffer solution and the characteristic substrate of peroxidase, carrying out uniform mixing so as to obtain a second mixed system and determining the light absorption value of the obtained mixture in the range of visible-light wavebands so as to realize detection of the organophosphorus pesticides in the test sample. The invention also discloses a detection kit for the organophosphorus pesticides. The detection kit comprises the characteristic substrate of peroxidase, the gold-core platinum-shell nanoparticles, polyacrylic acid, auxiliary reagents and the like. The detection method for the organophosphorus pesticides in the invention is simple, convenient, rapid, low in cost, high in stability and applicable to detection of residual organophosphorus pesticides in environment samples, food samples and the like.

Description

technical field [0001] The invention relates to an organophosphorus pesticide detection kit and its application, in particular to an organophosphorus pesticide detection method and organophosphorus pesticide detection kit based on nano-simulated enzyme activity regulation, belonging to the field of analytical chemistry. Background technique [0002] Enzymes are a class of biocatalysts, proteins with catalytic functions. Chemical reactions can be catalyzed in a suitable environment, but due to the inherent characteristics of enzymes, their applications are also limited, such as proteases are easy to denature and hydrolyze, and high costs and strict conditions of use also limit their applications. Therefore, it is particularly important to develop a mimetic enzyme with similar catalytic activity. [0003] Nanomimetic enzymes are a class of non-protein, artificially synthesized nanostructures that have catalytic properties similar to natural enzymes. Since 2007, Yan Xiyun et ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 彭池方魏新林施美荣
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap