Culture medium for tissue culture of toona ciliate roem.

A technology of tissue culture and culture medium, which is applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems such as no red toon tissue, and achieve the effect of neat growth, strong buds, and maintaining excellent traits

Inactive Publication Date: 2014-05-21
钦州市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the tissue culture of red toon

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The medium formula of red toon tissue culture, this medium comprises subculture medium and rooting medium, wherein:

[0059] The components and the content of each component per liter (L) of the subculture medium are as follows:

[0060] (1) Macroelements: potassium nitrate 1267mg / L, ammonium nitrate 1100mg / L, calcium chloride dihydrate 293mg / L, magnesium sulfate heptahydrate 247mg / L, potassium dihydrogen phosphate 113mg / L, ferrous sulfate heptahydrate 27.8mg / L, disodium edetate 37.3mg / L;

[0061] (2) Trace elements: manganese sulfate tetrahydrate 22.3mg / L, zinc sulfate heptahydrate 8.6mg / L, boric acid 6.2mg / L, sodium molybdate dihydrate 0.25mg / L, potassium iodide 0.83mg / L, copper sulfate pentahydrate 0.025mg / L, cobalt chloride hexahydrate 0.025mg / L;

[0062] (3) Organic matter: inositol 100mg / L, glycine 2.0mg / L, pyridoxine hydrochloride (VB 6 ) 0.5mg / L, niacin 0.5mg / L, thiamine hydrochloride (VB1) 0.1mg / L;

[0063] (4) Plant growth regulator: 6-benzyl adenine 0.4mg / ...

Embodiment 2

[0072] The medium formula of red toon tissue culture, this medium comprises subculture medium and rooting medium, wherein:

[0073] The components and the content of each component per liter (L) of the subculture medium are as follows:

[0074] (1) Macroelements: potassium nitrate 1267mg / L, ammonium nitrate 1100mg / L, calcium chloride dihydrate 293mg / L, magnesium sulfate heptahydrate 247mg / L, potassium dihydrogen phosphate 113mg / L, ferrous sulfate heptahydrate 27.8mg / L, disodium edetate 37.3mg / L;

[0075] (2) Trace elements: manganese sulfate tetrahydrate 22.3mg / L, zinc sulfate heptahydrate 8.6mg / L, boric acid 6.2mg / L, sodium molybdate dihydrate 0.25mg / L, potassium iodide 0.83mg / L, copper sulfate pentahydrate 0.025mg / L, cobalt chloride hexahydrate 0.025mg / L;

[0076] (3) Organic matter: inositol 100mg / L, glycine 2.0mg / L, pyridoxine hydrochloride (VB6) 0.5mg / L, niacin 0.5mg / L, thiamine hydrochloride (VB6) 1 ) 0.1mg / L;

[0077] (4) Plant growth regulator: 6-benzyl adenine 0....

Embodiment 3

[0086] The medium formula of red toon tissue culture, this medium comprises subculture medium and rooting medium, wherein:

[0087] The components and the content of each component per liter (L) of the subculture medium are as follows:

[0088] (1) Macroelements: potassium nitrate 1267mg / L, ammonium nitrate 1100mg / L, calcium chloride dihydrate 293mg / L, magnesium sulfate heptahydrate 247mg / L, potassium dihydrogen phosphate 113mg / L, ferrous sulfate heptahydrate 27.8mg / L, disodium edetate 37.3mg / L;

[0089] (2) Trace elements: manganese sulfate tetrahydrate 22.3mg / L, zinc sulfate heptahydrate 8.6mg / L, boric acid 6.2mg / L, sodium molybdate dihydrate 0.25mg / L, potassium iodide 0.83mg / L, copper sulfate pentahydrate 0.025mg / L, cobalt chloride hexahydrate 0.025mg / L;

[0090] (3) Organic matter: inositol 100mg / L, glycine 2.0mg / L, pyridoxine hydrochloride (VB 6 ) 0.5mg / L, niacin 0.5mg / L, thiamine hydrochloride (VB 1 ) 0.1mg / L;

[0091] (4) Plant growth regulator: 6-benzyl adenine 0...

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PUM

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Abstract

The invention discloses a culture medium for tissue culture of toona ciliate roem. The culture medium comprises a subculture medium and a rooting culture medium. A tissue culture manner is used for carrying out germchit vegetative propagation for the first time; compared with the prior art, the culture medium has the advantages that the seed propagation is rapid so that the good property of female parents can be effectively kept and the quality of germchits is guaranteed.

Description

technical field [0001] The invention relates to a tissue culture medium, in particular to a red toon tissue culture medium. Background technique [0002] Red toon (Toona ciliata Roem.), also known as neem, Indian toon, Mairong, Toona genus of Meliaceae, semi-evergreen tree, national second-level key protected wild plant, is a precious fast-growing timber tree species in tropical and subtropical regions of my country. Mainly distributed in Guangdong, Guangxi, Yunnan, Guizhou and Anhui provinces. In 1973, a small amount of natural distribution was discovered for the first time in Jingxian County, southern Anhui Province. Its wood is used for high-quality furniture and is known as "Chinese mahogany". Red toon is also a medicinal plant that has not yet been exploited. Studies in recent years have found that the extract of Toona sinensis has various biological activities such as antibacterial, antiviral, molluscicide and antimalarial. At present, the natural resources of this t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 何贵整陈丽文陈乃明蔡林梁刚时群樊东函钟焕祯王华宇杨利平韦冬玲
Owner 钦州市林业科学研究所
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